US4448884A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Glass-surface microcarrier for growth of cell cultures
Assignee: KMS Fusion Inc
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Filing: 1982-03-03
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Granted: 1984-05-15
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Expires: 2002-03-03
Abstract
Abstract A microcarrier for anchorage-dependent cell cultivation is prepared containing a spherical substrate of polymeric material having a bulk density of about 1 g/cc so as to be substantially buoyant in an aqueous culture medium, and a thin (less than 1 μm) coating layer of silicate glass. The silicate glass coating layer is preferably applied to a spherical precursor of polymeric material in an rf sputtering operation. An intermediate coating layer of magnetic material may be deposited prior to the silicate glass layer, so that the microcarriers may be readily removed from culture media by suitable subjection to a magnetic field.
Inventors
- 👤 Timothy M. Henderson
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-31 21:57
CPC Classifications
C; C12; C12N; C12N5/00; C12N5/0068; C12N5/0075; C12N2533/00; C12N2533/10; C12N2533/12; C12N2533/30
Claims Analysis
Total Claims: 9
Claim 1
Independent Claim
47 words
A microcarrier adapted for use as growth sites for anchorage dependent cells in an aqueous cell culture medium comprising a spherical substrate of polymeric material having a density of about 1 g/cc and a thin continuous discrete surface layer of silicate glass composition entirely surrounding said substrate.
Claim 2
Dependent Claim
47 words
A microcarrier adapted for use as growth sites for anchorage dependent cells in an aqueous cell culture medium comprising a spherical substrate of polymeric material having a density of about 1 g/cc and a thin continuous discrete surface layer of silicate glass composition entirely surrounding said substrate.
Claim 3
Dependent Claim
52 words
The microcarrier set forth in claim 1 wherein said substrate has a diameter in the range of 50 μm to 500 μm and a density in the range of 1.00 g/cc to 1.10 g/cc, and wherein said discrete surface layer has a thickness in the range of 300 Å to 1 μm.
Claim 4
Dependent Claim
26 words
The microcarrier set forth in claim 1 or 2 comprising a second discrete surface layer of magnetic material surrounding said substrate beneath said silicate glass layer.
Claim 5
Independent Claim
44 words
A method of fabricating a microcarrier for anchorage-dependent cell cultivation comprising the steps of: (a) selecting a spherical precursor of polymeric material having a density of about 1 g/cc, and (b) depositing on said precursor a discrete continuous surface layer of silicate glass composition.
Claim 6
Independent Claim
44 words
A method of fabricating a microcarrier for anchorage-dependent cell cultivation comprising the steps of: (a) selecting a spherical precursor of polymeric material having a density of about 1 g/cc, and (b) depositing on said precursor a discrete continuous surface layer of silicate glass composition.
Claim 7
Dependent Claim
18 words
The method set forth in claim 4 wherein said step (b) is accomplished in an rf sputtering operation.
Claim 8
Dependent Claim
45 words
The method set forth in claim 4 or 5 comprising the additional step prior to said step (b) of: (c) depositing on said precursor a discrete surface layer of magnetic material, said layer of silicate glass composition being deposited over said layer of magnetic material.
Claim 9
Dependent Claim
18 words
The method set forth in claim 6 wherein said step (c) is accomplished in an rf sputtering operation.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1987-05-15 | 1987-11-14 | 1988-05-15 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1991-05-15 | 1991-11-14 | 1992-05-15 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1995-05-15 | 1995-11-14 | 1996-05-15 | $7700.00 | Window Closed / Lapsed |