Patent US3923166A successfully refreshed from Google Patents.
US4442205A
Expired / Lapsed
US
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Simian virus recombinant that directs the synthesis of hepatitis B surface antigen
Assignee: United States Department of Health and Human Services
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Filing: 1981-09-22
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Granted: 1984-04-10
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Expires: 2001-09-22
Abstract
Abstract A process for producing a recombinant between simian virus 40 (SV40) and hepatitis B virus (HBV) is given. When tissue culture cells are infected with the recombinant, hepatitis B surface antigen is produced. Because a single cloned gene is used, the surface antigen produced is homogeneous and can be produced without conventional dependence on human sera. The antigen is excreted into the culture medium as 22 nm particles with the same physical properties, antigenic composition and constituent polypeptides as those found in the sera of patients with Type B hepatitis. The antigen is useful for the preparation of vaccines.
Inventors
- 👤 Dean H. Hamer
- 👤 John Gerin
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-24 09:44
CPC Classifications
C; C07; C07K; C07K14/00; C07K14/005; C12; C12N; C12N15/00; C12N15/09; C12N15/63; C12N15/79; C12N15/85; C12N15/86; A; A61; A61K; A61K39/00; C12N2730/00; C12N2730/00011; C12N2730/10011; C12N2730/10111; C12N2730/10122
Claims Analysis
Total Claims: 3
Claim 1
Independent Claim
145 words
A process for producing hepatitis B surface antigen comprising the steps of: (a) digesting HBV DNA and an E. coli plasmid with Bam HI and ligating the digest to produce E. coli plasmid-HBV recombinant (b) cleaving the resulting plasmid-HBV with Bam HI to produce a 1350 bp DNA fragment containing the HB s Ag coding region (c) preparing recombinant plasmid-SV40 by ligating a Bam HI Eco RI digest of E. coli plasmid and SV40 (d) said 1350 bp DNA fragment is then ligated to Bam HI cleaved plasmid-SV40 to produce double recombinant E. coli plasmid-SV40-HBV (e) said double recombinant plasmid is then cleaved with Hae II to generate 4950 bp linear SV40-HBV recombinant molecules (f) infecting monkey kidney cells with the 4950 bp molecules and SV40 temperature sensitive early gene mutant helper virus and (g) culturing the infected cells to produce hepatitis B surface antigen.
Claim 2
Dependent Claim
145 words
A process for producing hepatitis B surface antigen comprising the steps of: (a) digesting HBV DNA and an E. coli plasmid with Bam HI and ligating the digest to produce E. coli plasmid-HBV recombinant (b) cleaving the resulting plasmid-HBV with Bam HI to produce a 1350 bp DNA fragment containing the HB s Ag coding region (c) preparing recombinant plasmid-SV40 by ligating a Bam HI Eco RI digest of E. coli plasmid and SV40 (d) said 1350 bp DNA fragment is then ligated to Bam HI cleaved plasmid-SV40 to produce double recombinant E. coli plasmid-SV40-HBV (e) said double recombinant plasmid is then cleaved with Hae II to generate 4950 bp linear SV40-HBV recombinant molecules (f) infecting monkey kidney cells with the 4950 bp molecules and SV40 temperature sensitive early gene mutant helper virus and (g) culturing the infected cells to produce hepatitis B surface antigen.
Claim 3
Dependent Claim
18 words
The double recombinant plasmid produced by the process of claim 1 wherein the E. coli plasmid is pBR322.
References Cited (Backward Citations)
No citation records indexed.
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1987-04-10 | 1987-10-10 | 1988-04-10 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1991-04-10 | 1991-10-10 | 1992-04-10 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1995-04-10 | 1995-10-10 | 1996-04-10 | $7700.00 | Window Closed / Lapsed |