US4440860A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Stimulating cell growth
Assignee: Boston Children's Hospital
•
Filing: 1981-03-02
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Granted: 1984-04-03
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Expires: 2001-03-02
Abstract
Abstract Compositions and methods for promoting cell growth featuring, in one aspect, cell culture media containing milk or colostrum and fibronectin; fibronectin is preferably pre-coated onto the culture substrate.
Inventors
- 👤 Michael Klagsbrun
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-26 00:07
CPC Classifications
C; C12; C12N; C12N5/00; C12N5/0068; C12N2533/00; C12N2533/50; C12N2533/52
Claims Analysis
Total Claims: 41
Claim 1
Independent Claim
9 words
A cell culture medium comprising mammalian milk and fibronectin.
Claim 2
Dependent Claim
9 words
A cell culture medium comprising mammalian milk and fibronectin.
Claim 3
Dependent Claim
22 words
The medium of claim 1, further comprising at least one of the additives transferrin, insulin, epidermal growth factor, and fibroblast growth factor.
Claim 4
Dependent Claim
18 words
The medium of claim 1 wherein said milk is bovine milk, and said fibronectin is human plasma fibronectin.
Claim 5
Dependent Claim
28 words
The cell culture medium of any one of claims 1, 2, or 3, wherein said mammalian milk comprises, by volume, between about 0.5% and 25% of said medium.
Claim 6
Dependent Claim
17 words
The medium of claim 4 wherein said mammalian milk comprises, by volume, about 10% of said medium.
Claim 7
Dependent Claim
8 words
A cell culture medium comprising colostrum and fibronectin.
Claim 8
Dependent Claim
8 words
A cell culture medium comprising colostrum and fibronectin.
Claim 9
Dependent Claim
22 words
The medium of claim 6, further comprising at least one of the additives transferrin, insulin, epidermal growth factor, and fibroblast growth factor.
Claim 10
Dependent Claim
18 words
The medium of claim 6 wherein said colostrum is bovine colostrum, and said fibronectin is human plasma fibronectin.
Claim 11
Independent Claim
24 words
The medium of any one of claims 6, 7, or 8, wherein said colostrum comprises, by volume, between 0.5% and 25% of said medium.
Claim 12
Dependent Claim
18 words
The medium of claim 9 wherein said colostrum comprises, by volume, between 2% and 5% of said medium.
Claim 13
Dependent Claim
16 words
The medium of claim 10 wherein said colostrum comprises, by volume, about 5% of said medium.
Claim 14
Dependent Claim
16 words
The medium of claim 10 wherein said colostrum comprises, by volume, about 2% of said medium.
Claim 15
Dependent Claim
12 words
The medium of claim 12 wherein said medium contains insulin and transferrin.
Claim 16
Dependent Claim
23 words
The medium of claim 13 wherein said insulin and transferrin are each present in said medium in the approximate concentration of 10 μg/ml.
Claim 17
Dependent Claim
20 words
A cell culture means comprising nutrient medium comprising mammalian milk, and a cell culture surface which is precoated with fibronectin.
Claim 18
Dependent Claim
20 words
A cell culture means comprising nutrient medium comprising mammalian milk, and a cell culture surface which is precoated with fibronectin.
Claim 19
Dependent Claim
20 words
The cell culture means of claim 15 wherein said milk is bovine milk, and said fibronectin is human plasma fibronectin.
Claim 20
Dependent Claim
19 words
A cell culture means comprising nutrient medium comprising colostrum, and a cell culture surface which is pre-coated with fibronectin.
Claim 21
Dependent Claim
19 words
A cell culture means comprising nutrient medium comprising colostrum, and a cell culture surface which is pre-coated with fibronectin.
Claim 22
Dependent Claim
20 words
The cell culture means of claim 17 wherein said colostrum is bovine colostrum, and said fibronectin is human plasma fibronectin.
Claim 23
Dependent Claim
39 words
A process of culturing cells comprising providing a cell culture substrate which is coated with fibronectin, introducing said cells onto said substrate, and providing the proper conditions for growth, including feeding said cells with nutrient medium comprising mammalian milk.
Claim 24
Dependent Claim
39 words
A process of culturing cells comprising providing a cell culture substrate which is coated with fibronectin, introducing said cells onto said substrate, and providing the proper conditions for growth, including feeding said cells with nutrient medium comprising mammalian milk.
Claim 25
Dependent Claim
19 words
The process of claim 19 wherein said mammalian milk comprises by volume, between 0.5% and 25% of said medium.
Claim 26
Dependent Claim
17 words
The process of claim 20 wherein said mammalian milk comprises, by volume, about 10% of said medium.
Claim 27
Dependent Claim
31 words
The process of any one of claims 19, 20, or 21 wherein said medium further comprises at least one of the additives transferrin, insulin, epidermal growth factor, and fibroblast growth factor.
Claim 28
Dependent Claim
38 words
A process of culturing cells comprising providing a cell culture substrate which is coated with fibronectin, introducing said cells onto said substrate, and providing the proper conditions for growth, including feeding said cells with nutrient medium comprising colostrum.
Claim 29
Dependent Claim
38 words
A process of culturing cells comprising providing a cell culture substrate which is coated with fibronectin, introducing said cells onto said substrate, and providing the proper conditions for growth, including feeding said cells with nutrient medium comprising colostrum.
Claim 30
Dependent Claim
18 words
The process of claim 23 wherein said colostrum comprises, by volume, between 0.5% and 20% of said medium.
Claim 31
Dependent Claim
16 words
The process of claim 24 wherein said colostrum comprises, by volume, about 2% of said medium.
Claim 32
Dependent Claim
16 words
The process of claim 24 wherein said colostrum comprises, by volume, about 5% of said medium.
Claim 33
Dependent Claim
32 words
The process of any one of claims 23, 24, 25, or 26 wherein said medium further comprises at least one of the additives transferrin, insulin, epidermal growth factor, and fibroblast growth factor.
Claim 34
Independent Claim
22 words
A method of promoting proliferation of transformed or cancerous cells, said method comprising feeding said cells medium containing colostrum or mammalian milk.
Claim 35
Independent Claim
22 words
A method of promoting proliferation of transformed or cancerous cells, said method comprising feeding said cells medium containing colostrum or mammalian milk.
Claim 36
Dependent Claim
19 words
The method of claim 28 wherein said medium comprises colostrum in an amount between 0.5% and 25% (by volume).
Claim 37
Dependent Claim
20 words
The method of claim 28 wherein said medium comprises mammalian milk in an amount between 1% and 25% (by volume).
Claim 38
Dependent Claim
11 words
A transformed or cancerous cell-proliferation medium comprising colostrum or mammalian milk.
Claim 39
Dependent Claim
11 words
A transformed or cancerous cell-proliferation medium comprising colostrum or mammalian milk.
Claim 40
Dependent Claim
16 words
The medium of claim 31 wherein said medium comprises between 0.5% and 25% (by volume) colostrum.
Claim 41
Dependent Claim
17 words
The medium of claim 31 wherein said medium comprises between 1.0% and 25% (by volume) mammalian milk.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1987-04-03 | 1987-10-03 | 1988-04-03 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1991-04-03 | 1991-10-03 | 1992-04-03 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1995-04-03 | 1995-10-03 | 1996-04-03 | $7700.00 | Window Closed / Lapsed |