US4438204A
Expired / Lapsed
US
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Determination of glycosilated hemoglobin
Assignee: Roche Diagnostics GmbH
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Filing: 1982-09-29
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Granted: 1984-03-20
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Expires: 2002-09-29
Abstract
Abstract A process for the determination of glycosilated hemoglobin in a blood sample comprises liberating glycosilated and non-glycosilated hemoglobin from the erythrocytes by chemical or physical treatment; converting, if desired, the hemoglobin into methemoglobin, and thereafter differentiating the glycosilated and non-glycosilated hemoglobin portion using its reaction with haptoglobin, and determining the glycosilated and/or the non-glycosilated hemoglobin portion. The present invention also provides a reagent for the determination of glycosilated hemoglobin in a blood sample, as well as a reagent for the differentiation of glycosilated and non-glycosilated hemoglobin, comprising haptoglobin.
Inventors
- 👤 Rolf Deeg
- 👤 Urban Schmitt
- 👤 Joachim Ziegenhorn
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-10 02:29
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/72; G01N33/721; G01N33/723; Y; Y10; Y10T; Y10T436/00; Y10T436/10; Y10T436/107497
Claims Analysis
Total Claims: 14
Claim 1
Independent Claim
58 words
A process for the determination of glycosilated hemoglobin in a blood sample containing hemoglobin comprised of both glycosilated and non-glycosilated hemoglobin comprising the steps liberating the glycosilated and non-glycosilated hemoglobin from erythrocytes in the blood sample, and differentiating the glycosilated and non-glycosilated hemoglobin by reaction with haptoglobin, and determining the glycosilated and/or the non-glycosilated portion of the hemoglobin.
Claim 2
Dependent Claim
58 words
A process for the determination of glycosilated hemoglobin in a blood sample containing hemoglobin comprised of both glycosilated and non-glycosilated hemoglobin comprising the steps liberating the glycosilated and non-glycosilated hemoglobin from erythrocytes in the blood sample, and differentiating the glycosilated and non-glycosilated hemoglobin by reaction with haptoglobin, and determining the glycosilated and/or the non-glycosilated portion of the hemoglobin.
Claim 3
Dependent Claim
16 words
The process of claim 1 wherein the hemoglobin is converted to methemoglobin before reacting with haptoglobin.
Claim 4
Dependent Claim
21 words
The process of claim 1, wherein the differentiation of glycosilated and non-glycosilated hemoglobin is accomplished in the presence of buffer system.
Claim 5
Dependent Claim
32 words
The process of claim 1, wherein the differentiation of glycosilated and non-glycosilated hemoglobin is accomplished in the presence of one or more substances which bring about a conformation change in the hemoglobin.
Claim 6
Dependent Claim
33 words
The process according to claim 4, wherein the substances which bring about a conformation change in the hemoglobin are either compounds with a binding action on the allosteric effector places or heme-binding ligands.
Claim 7
Dependent Claim
25 words
The process according to claim 5, wherein the compound with a binding action on the allosteric effector places is inositol hexaphosphate, 2,3-diphosphoglycerate or mellitic acid.
Claim 8
Dependent Claim
20 words
The process according to claim 5, wherein the heme-binding ligand is an alkyl isocyanide, oxygen, carbon monoxide or nitrogen monoxide.
Claim 9
Dependent Claim
31 words
The process of claim 2 wherein the differentiation of glycosilated and non-glycosilated hemoglobin is accomplished in the presence of one or more substances which bring about conformational change in the hemoglobin.
Claim 10
Dependent Claim
19 words
The process of claim 8 wherein the substance for effecting conformational change is a fluoride, azide, cyanide or water.
Claim 11
Dependent Claim
25 words
The process of claim 1 wherein a 2 to 50 fold excess of the amount of haptoglobin necessary to react with the hemoglobin, is used.
Claim 12
Dependent Claim
36 words
Reagent for the determination of glycosilated hemoglobin in a blood sample, comprising a chemical agent for hemolysing erythrocytes, a buffer system effective in the pH range of from 4.0 to 8.5, and free or carrier-bound haptoglobin.
Claim 13
Dependent Claim
36 words
Reagent for the determination of glycosilated hemoglobin in a blood sample, comprising a chemical agent for hemolysing erythrocytes, a buffer system effective in the pH range of from 4.0 to 8.5, and free or carrier-bound haptoglobin.
Claim 14
Dependent Claim
41 words
Reagent of claim 11 further comprising one or more substances with a binding action on the allosteric effector on places of the hemoglobin and one or more heme-binding ligands and/or one or more substances with a stabilizing action on ionic bonds.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1987-03-20 | 1987-09-19 | 1988-03-20 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1991-03-20 | 1991-09-19 | 1992-03-20 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1995-03-20 | 1995-09-19 | 1996-03-20 | $7700.00 | Window Closed / Lapsed |