US4434227A
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Immunoassay for class specific immunoglobulin antibodies
Assignee: Abbott Laboratories
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Filing: 1982-02-08
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Granted: 1984-02-28
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Expires: 2002-02-08
Abstract
Abstract A method for determining an immunoglobulin of the IgX class in a sample where X is either M, A, D or E. Anti-IgG is added to IgG to prevent binding of rheumatoid factor before the sample containing IgX is added to insolubilized IgG.
Inventors
- 👤 John T. Unger
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-30 23:01
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/68; G01N33/6854; Y; Y10; Y10S; Y10S435/00; Y10S435/962; Y10S436/00; Y10S436/825
Claims Analysis
Total Claims: 25
Claim 1
Independent Claim
104 words
A method for determining an immunoglobulin antibody of an IgX class in a sample, wherein X is selected from the group consisting of M, A, D and E, comprising: (a) treating the sample with an effective amount of anti-IgG; (b) contacting the treated sample with a class specific antibody reagent comprising an antigen, for which the immunoglobulin antibody IgX is specific, coated on a solid support; to form an antigen-IgX complex on the solid support; (c) removing unbound sample; (d) treating the antigen-IgX complex with anti-IgX; and (e) determining the anti-IgX bound to the antigen-IgX complex as a measure of IgX in the sample.
Claim 2
Independent Claim
104 words
A method for determining an immunoglobulin antibody of an IgX class in a sample, wherein X is selected from the group consisting of M, A, D and E, comprising: (a) treating the sample with an effective amount of anti-IgG; (b) contacting the treated sample with a class specific antibody reagent comprising an antigen, for which the immunoglobulin antibody IgX is specific, coated on a solid support; to form an antigen-IgX complex on the solid support; (c) removing unbound sample; (d) treating the antigen-IgX complex with anti-IgX; and (e) determining the anti-IgX bound to the antigen-IgX complex as a measure of IgX in the sample.
Claim 3
Dependent Claim
25 words
A method according to claim 1 wherein the amount of anti-IgG of step (a) is immunochemically greater than the amount of IgG in the sample.
Claim 4
Dependent Claim
14 words
A method according to claim 2 wherein the anti-IgX is labeled with an enzyme.
Claim 5
Dependent Claim
23 words
A method according to claim 3 wherein unbound labeled anti-IgX is removed prior to determining the labeled anti-IgX bound to the antigen-IgX complex.
Claim 6
Dependent Claim
17 words
A method according to claim 1 or 4 wherein IgX is an IgM antibody against rubella virus.
Claim 7
Dependent Claim
25 words
A method according to claim 1 wherein step (c) is preformed by aspiration of the unbound sample and washing of the solid support with water.
Claim 8
Independent Claim
111 words
A method for determining an immunoglobulin antibody of an IgX class in a sample, wherein X is selected from the group consisting of M, A, D and E, comprising: (a) treating the sample with an effective amount of anti-IgG; (b) contacting the treated sample with a class specific antibody reagent comprising an antigen, for which the immunoglobulin antibody IgX is specific, coated on a solid support; to form an antigen-IgX complex on the solid support; (c) removing unbound sample; (d) treating the antigen-IgX complex with labeled anti-IgX; (e) removing unbound labeled anti-IgX; and (f) determining the labeled anti-IgX bound to the antigen-IgX complex as a measure of IgX in the sample.
Claim 9
Independent Claim
111 words
A method for determining an immunoglobulin antibody of an IgX class in a sample, wherein X is selected from the group consisting of M, A, D and E, comprising: (a) treating the sample with an effective amount of anti-IgG; (b) contacting the treated sample with a class specific antibody reagent comprising an antigen, for which the immunoglobulin antibody IgX is specific, coated on a solid support; to form an antigen-IgX complex on the solid support; (c) removing unbound sample; (d) treating the antigen-IgX complex with labeled anti-IgX; (e) removing unbound labeled anti-IgX; and (f) determining the labeled anti-IgX bound to the antigen-IgX complex as a measure of IgX in the sample.
Claim 10
Dependent Claim
15 words
A method according to claim 7 wherein the labeled anti-IgX is labeled with an enzyme.
Claim 11
Dependent Claim
15 words
A method according to claim 8 wherein IgX is an IgM antibody against rubella virus.
Claim 12
Dependent Claim
29 words
A method according to claim 7 wherein steps (c) and (e) are preformed by aspiration of the unbound sample and labeled anti-IgX respectively and washing of the solid support.
Claim 13
Independent Claim
126 words
A method for determining an immunoglobulin antibody of an IgX class in a sample, wherein X is selected from the group consisting of M, A, D and E, comprising: (a) treating the sample with an effective amount of anti-IgG; (b) contacting the treated sample with a class specific antibody reagent comprising an antigen, for which the immunoglobulin antibody IgX is specific, coated on a solid support; to form an antigen-IgX complex on the solid support; (c) removing unbound sample; (d) treating the antigen-IgX complex with anti-IgX; (e) removing unbound anti-IgX; (f) treating the antigen-IgX-anti-IgX with labeled antibody to anti-IgX; (g) removing unbound labeled antibody to the anti-IgX; and (h) determining the labeled antibody bound to the antigen-IgX-anti-Igx complex as a measure of IgX in the sample.
Claim 14
Independent Claim
126 words
A method for determining an immunoglobulin antibody of an IgX class in a sample, wherein X is selected from the group consisting of M, A, D and E, comprising: (a) treating the sample with an effective amount of anti-IgG; (b) contacting the treated sample with a class specific antibody reagent comprising an antigen, for which the immunoglobulin antibody IgX is specific, coated on a solid support; to form an antigen-IgX complex on the solid support; (c) removing unbound sample; (d) treating the antigen-IgX complex with anti-IgX; (e) removing unbound anti-IgX; (f) treating the antigen-IgX-anti-IgX with labeled antibody to anti-IgX; (g) removing unbound labeled antibody to the anti-IgX; and (h) determining the labeled antibody bound to the antigen-IgX-anti-Igx complex as a measure of IgX in the sample.
Claim 15
Dependent Claim
25 words
A method according to claim 11 wherein the amount of anti-IgG of step (a) is immunochemically greater than the amount of IgG in the sample.
Claim 16
Dependent Claim
17 words
A method according to claim 11 wherein the labeled antibody to anti-IgX is labeled with an enzyme.
Claim 17
Dependent Claim
15 words
A method according to claim 13 wherein IgX is an IgM antibody against rubella virus.
Claim 18
Dependent Claim
36 words
A method according to claim 11 wherein steps (c), (e) and (g) are preformed by aspiration of the unbound sample, unbound labeled anti-IgX and unbound labeled antibody to anti-IgX repsectively and washing of the solid support.
Claim 19
Independent Claim
94 words
A method for determining an immunoglobulin antibody of an IgG class in a sample comprising: (a) treating the sample with an effective amount of anti-IgG; (b) contacting the treated sample with a class specific antibody reagent comprising an antigen for which the immunoglobulin antibody IgG is specific, coated on a solid support; to form an antigen-IgG-anti-IgG complex on the solid support; (c) removing unbound sample; (d) treating the antigen-IgG-anti-IgG complex with antibody to anti-IgG; and (e) determining the antibody to anti-IgG bound to the antigen-IgG-anti-IgG complex as a measure of IgG in the sample.
Claim 20
Independent Claim
94 words
A method for determining an immunoglobulin antibody of an IgG class in a sample comprising: (a) treating the sample with an effective amount of anti-IgG; (b) contacting the treated sample with a class specific antibody reagent comprising an antigen for which the immunoglobulin antibody IgG is specific, coated on a solid support; to form an antigen-IgG-anti-IgG complex on the solid support; (c) removing unbound sample; (d) treating the antigen-IgG-anti-IgG complex with antibody to anti-IgG; and (e) determining the antibody to anti-IgG bound to the antigen-IgG-anti-IgG complex as a measure of IgG in the sample.
Claim 21
Dependent Claim
25 words
A method according to claim 16 wherein the amount of anti-IgG of step (a) is immunochemically greater than the amount of IgG in the sample.
Claim 22
Dependent Claim
16 words
A method according to claim 16 wherein the antibody to anti-IgG is labeled with an enzyme.
Claim 23
Dependent Claim
29 words
A method according to claim 18 wherein step (e) unbound labeled antibody to IgG is removed prior to determinating the amount of bound antibody to anti-IgG in the sample.
Claim 24
Dependent Claim
23 words
A method according to claim 19 wherein the unbound labeled antibody to IgG is removed by aspiration and washing of the solid support.
Claim 25
Dependent Claim
22 words
A method according to claim 16 wherein step (c) is preformed by aspiration of the unbound sample and washing of the bead.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1987-02-27 | 1987-08-29 | 1988-02-28 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1991-02-27 | 1991-08-29 | 1992-02-28 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1995-02-27 | 1995-08-29 | 1996-02-28 | $7700.00 | Window Closed / Lapsed |