US4431633A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Influenza vaccine
Assignee: Merck and Co Inc
•
Filing: 1982-04-27
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Granted: 1984-02-14
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Expires: 2002-04-27
Abstract
Abstract Influenza virus is extracted with a mixture of ether and ethanol, the aqueous and organic phases are separated and residual ether removed from the virus suspension under reduced pressure. The resulting product is useful as an influenza vaccine.
Inventors
- 👤 Roy A. Machlowitz
- 👤 Alan C. Herman
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-05 19:08
CPC Classifications
A; A61; A61K; A61K39/00; A61K39/12; A61K39/145; A61K2039/51; A61K2039/525; A61K2039/5252; A61K2039/5254; C; C12; C12N; C12N2760/00; C12N2760/00011; C12N2760/16011; C12N2760/16111; C12N2760/16134; C12N2760/16211; C12N2760/16234
Claims Analysis
Total Claims: 7
Claim 1
Independent Claim
34 words
A method for preparing an immunogenic influenza vaccine which comprises extracting aqueous live influenza virus with a mixture containing from about 98% to about 90% ether and from about 2% to about 10% ethanol.
Claim 2
Independent Claim
34 words
A method for preparing an immunogenic influenza vaccine which comprises extracting aqueous live influenza virus with a mixture containing from about 98% to about 90% ether and from about 2% to about 10% ethanol.
Claim 3
Dependent Claim
22 words
A method according to claim 1 wherein the extraction takes place at a temperature of from about 5° to about 25° C.
Claim 4
Dependent Claim
25 words
A method according to claim 1 wherein the ether layer is discarded and the inactivated yet immunogenic influenza virus is recovered from the aqueous phase.
Claim 5
Independent Claim
45 words
A method for preparing an immunogenic influenza vaccine which comprises extracting live influenza virus with a mixture comprising from about 0.5 to about 5 volumes of diethyl ether plus from about 0.02 to about 0.1 volume of ethanol per volume of live aqueous influenza virus.
Claim 6
Independent Claim
45 words
A method for preparing an immunogenic influenza vaccine which comprises extracting live influenza virus with a mixture comprising from about 0.5 to about 5 volumes of diethyl ether plus from about 0.02 to about 0.1 volume of ethanol per volume of live aqueous influenza virus.
Claim 7
Dependent Claim
33 words
A method according to claim 4 wherein the mixture comprises about 1 volume of diethyl ether plus from about 0.02 to about 0.1 volume of ethanol per volume of live aqueous influenza virus.
References Cited (Backward Citations)
US3031378A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1987-02-13 | 1987-08-15 | 1988-02-14 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1991-02-13 | 1991-08-15 | 1992-02-14 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1995-02-13 | 1995-08-15 | 1996-02-14 | $7700.00 | Window Closed / Lapsed |