US4429044A
Expired / Lapsed
US
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Preparation of an alkaline protease from flavobacterium arborescens
Assignee: Genencor International Indiana Inc
•
Filing: 1982-09-28
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Granted: 1984-01-31
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Expires: 2002-09-28
Abstract
Abstract The production of an alkaline serine protease by a soil bacterium, Flavobacterium arborescens is disclosed. Also disclosed is the use of the protease as an enzyme detergent.
Inventors
- 👤 George Boguslawski
- 👤 Ernest W. Boyer
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-26 07:26
CPC Classifications
C; C12; C12N; C12N9/00; C12N9/14; C12N9/48; C12N9/50; C12N9/52; Y; Y10; Y10S; Y10S435/00; Y10S435/8215; Y10S435/822; Y10S435/85
Claims Analysis
Total Claims: 9
Claim 1
Independent Claim
38 words
A method for the production of microbial alkaline protease which comprises cultivating an organism from the species Flavobacterium arborescens in an aqueous nutrient medium containing appropriate nutrients for a time sufficient to produce a recoverable quantity of enzyme.
Claim 2
Independent Claim
38 words
A method for the production of microbial alkaline protease which comprises cultivating an organism from the species Flavobacterium arborescens in an aqueous nutrient medium containing appropriate nutrients for a time sufficient to produce a recoverable quantity of enzyme.
Claim 3
Dependent Claim
36 words
The method of claim 1 wherein the nutrient medium contains 0.05 to 0.2% yeast extract, 0.5 to 2.0% xylose, 5 to 10% corn steep liquor and 0.5 to 1.0% potassium phosphate all on a weight/volume basis.
Claim 4
Dependent Claim
23 words
The method of claim 2 wherein the pH of the nutrient medium is adjusted to a level of from about 6.8 to 7.2.
Claim 5
Dependent Claim
27 words
The method of claim 1 wherein the organism is cultivated at a temperature of 29° to 32° C. for a period of from 16 to 24 hours.
Claim 6
Dependent Claim
15 words
The method of claim 1 wherein the protease producing organism is F. arborescens NRRL B-11,022.
Claim 7
Dependent Claim
15 words
The method of claim 1 wherein the protease producing organism is F. arborescens ATCC 4358.
Claim 8
Dependent Claim
25 words
The method of claim 1 wherein the organism cells are removed from the nutrient medium by centrifugation to leave a clear supernatant containing the protease.
Claim 9
Dependent Claim
35 words
The method of claim 7 wherein the protease is purified to homogeneity by addition of ammonium sulfate to the supernatant with precipitation and concentration followed by ion exchange chromatography on diethylaminoethyl-cellulose and sucrose gradient centrifugation.
References Cited (Backward Citations)
US4061539A
Cited
US4283496A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1987-01-30 | 1987-08-01 | 1988-01-31 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1991-01-30 | 1991-08-01 | 1992-01-31 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1995-01-30 | 1995-08-01 | 1996-01-31 | $7700.00 | Window Closed / Lapsed |