US4427892A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Detection and determination of species by fluorescence measurements
Assignee: National Research Development Corp UK
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Filing: 1980-06-06
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Granted: 1984-01-24
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Expires: 2000-06-06
Abstract
Abstract A fluorimetric detector for use in detecting or determining a species contained in the flowing liquid eluant of a chromatographic separation, comprising: a 147 Pm or 63 Ni source of beta-particles; a light-sensitive detection means, spaced from the source, for detecting visible fluorescent radiation generated in the eluant by the action thereon of beta-particles and for measuring a count rate of said fluorescent radiation; and flow-causing means to cause the eluant to flow between the source and the detection means is disclosed. Also disclosed, is a method of detecting or determining a species contained in the flowing liquid eluant of a chromatographic separation, the species being either a fluorescent species or a species which quenches fluorescence.
Inventors
- 👤 David J. Malcolme-Lawes
- 👤 Lawrence A. Gifford
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-08 02:45
CPC Classifications
G; G01; G01N; G01N23/00; G01N23/22; G01N23/223; G01N30/00; G01N30/02; G01N30/62; G01N30/74; G01N2223/00; G01N2223/07; G01N2223/076
Claims Analysis
Total Claims: 11
Claim 1
Independent Claim
102 words
A method of detecting or determining a species contained in the flowing liquid eluant of a chromatographic separation, which comprises providing a 147 Pm or 63 Ni source of beta-particles, generating visible fluorescent radiation in the eluant by the action of the beta-particles thereon, causing the eluant to flow between the source of beta-particles and a light-sensitive detection means, so that flluorescent radiation generated in the eluant is detected, and a count rate thereof is measured, by the detection means, optically filtering said radiation to select particular wavelengths for detection by said detection means and monitoring the output of the detection means.
Claim 2
Independent Claim
102 words
A method of detecting or determining a species contained in the flowing liquid eluant of a chromatographic separation, which comprises providing a 147 Pm or 63 Ni source of beta-particles, generating visible fluorescent radiation in the eluant by the action of the beta-particles thereon, causing the eluant to flow between the source of beta-particles and a light-sensitive detection means, so that flluorescent radiation generated in the eluant is detected, and a count rate thereof is measured, by the detection means, optically filtering said radiation to select particular wavelengths for detection by said detection means and monitoring the output of the detection means.
Claim 3
Dependent Claim
53 words
A method according to claim 1 wherein the concentration of the species to be detected or determined in the eluant is such that the fluorescent count, obtainable by integration from a plot of fluorescent count rate against time, is substantially linearly proportional to the amount of the species to be detected or determined.
Claim 4
Dependent Claim
25 words
A method according to claim 1 in which the eluant contains a species to be detected or determined which fluoresces when exposed to beta particles.
Claim 5
Dependent Claim
34 words
A method according to claim 1 in which the eluant contains a component which fluoresces when exposed to beta particles, and also contains a species to be detected or determined which quenches that fluorescence.
Claim 6
Dependent Claim
25 words
A method according to claim 1 in which the eluant is caused to flow through a cell of active volume between 1.5 and 10 microliters.
Claim 7
Dependent Claim
102 words
A fluorimetric detector for use in detecting or determining a species contained in the flowing liquid eluant of a chromatographic separation, comprising a 147 Pm or 63 Ni source of beta particles; a light-sensitive detection means, spaced from the source, for detecting visible fluorescent radiation generated in the eluant by the action thereon of beta-particles and for measuring a count rate of said fluorescent radiation; an optical filter between the liquid eluant and the detection means to select particular wavelengths for detection by said detection means and flow-causing means to cause the eluant to flow between the source and the detection means.
Claim 8
Dependent Claim
102 words
A fluorimetric detector for use in detecting or determining a species contained in the flowing liquid eluant of a chromatographic separation, comprising a 147 Pm or 63 Ni source of beta particles; a light-sensitive detection means, spaced from the source, for detecting visible fluorescent radiation generated in the eluant by the action thereon of beta-particles and for measuring a count rate of said fluorescent radiation; an optical filter between the liquid eluant and the detection means to select particular wavelengths for detection by said detection means and flow-causing means to cause the eluant to flow between the source and the detection means.
Claim 9
Dependent Claim
35 words
A fluorimetric detector according to claim 6 in which the flow-causing means comprises a flow cell between the source and the detector, the active volume of the flow cell being between 1.5 and 10 microliters.
Claim 10
Dependent Claim
16 words
A fluorimetric detector according to claim 6 in which the light-sensitive detection means is a photomultiplier.
Claim 11
Dependent Claim
32 words
A fluorimetric detector according to claim 6, further comprising recording means to monitor an electrical output signal from the detection means which is proportional to the count rate of the fluorescent radiation.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1987-01-23 | 1987-07-25 | 1988-01-24 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1991-01-23 | 1991-07-25 | 1992-01-24 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1995-01-23 | 1995-07-25 | 1996-01-24 | $7700.00 | Window Closed / Lapsed |