US4426357A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Antibody elution reagent kit
Assignee: Ortho Clinical Diagnostics Inc
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Filing: 1981-09-09
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Granted: 1984-01-17
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Expires: 2001-09-09
Abstract
Abstract A reagent kit for eluting antibodies present on the surface of red blood cells comprises sorbitan trioleate or other variants within the sorbitan family and a red blood cell diluent as well as methods for use.
Inventors
- 👤 Sherry Buffington
- 👤 Leonard T. Wilson
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-27 11:50
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/531; Y; Y10; Y10S; Y10S436/00; Y10S436/807; Y10S436/808
Claims Analysis
Total Claims: 4
Claim 1
Independent Claim
70 words
A reagent kit for eluting, in the presence of heat and agitation, antibodies present on the surface of red blood cells consisting essentially of: a first container containing an ester selected from the group consisting of sorbitan trioleate, sorbitan monoleate, and sorbitan monolaureate; and a second container containing a red blood cell diluent selected from the group consisting of saline, buffered saline, bovine serum albumin, and buffered red cell diluent.
Claim 2
Dependent Claim
70 words
A reagent kit for eluting, in the presence of heat and agitation, antibodies present on the surface of red blood cells consisting essentially of: a first container containing an ester selected from the group consisting of sorbitan trioleate, sorbitan monoleate, and sorbitan monolaureate; and a second container containing a red blood cell diluent selected from the group consisting of saline, buffered saline, bovine serum albumin, and buffered red cell diluent.
Claim 3
Dependent Claim
53 words
A reagent kit for eluting, in the presence of heat and agitation, antibodies present on the surface of red blood cells consisting essentially of: a first container containing an effective amount of sorbitan trioleate for disassociating substantially all adsorbed antibodies from the cells' surface and a second container containing buffered red cell diluent.
Claim 4
Dependent Claim
53 words
A reagent kit for eluting, in the presence of heat and agitation, antibodies present on the surface of red blood cells consisting essentially of: a first container containing an effective amount of sorbitan trioleate for disassociating substantially all adsorbed antibodies from the cells' surface and a second container containing buffered red cell diluent.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1987-01-16 | 1987-07-18 | 1988-01-17 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1991-01-16 | 1991-07-18 | 1992-01-17 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1995-01-16 | 1995-07-18 | 1996-01-17 | $7700.00 | Window Closed / Lapsed |