US4423034A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Process for the preparation of antibodies
Assignee: Toyo Jozo KK
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Filing: 1982-04-28
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Granted: 1983-12-27
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Expires: 2002-04-28
Abstract
Abstract A novel peptide of the formula H--Ser--Arg--Arg--Ala--Gln--Asp--Phe--Val--Gln--Trp--Leu--Met--Asn--Thr--OH is the 16-29 fragment of glucagon and can be conjugated with a protein such as albumin or modified albumin using glutaraldehyde or 3-(2'-benzothiazolyl-dithio) propionate succinimide ester as a conjugation reagent. The conjugated peptide can be used to sensitize a mammal by administering the conjugated peptide subcutaneously, after which the blood is collected from the mammal and antibodies concentrated from the blood, which antibodies are then useful in assay methods for trace amounts of glucagon in human blood.
Inventors
- 👤 Nobuaki Nakagawa
- 👤 Kikuo Kotani
- 👤 Shigeo Katsuragi
- 👤 Kaoru Morita
- 👤 Kunio Ohyama
- 👤 Toshiharu Noda
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 15:20
CPC Classifications
C; C07; C07K; C07K14/00; C07K14/435; C07K14/575; C07K14/605
Claims Analysis
Total Claims: 3
Claim 1
Independent Claim
44 words
A process for the preparation of specific antibodies which comprises sensitizing a mammal by administering to said mammal a peptide of the formula H--Ser--Arg--Arg--Ala--Gln--Asp--Phe--Val--Gln--Trp--Leu--Met--Asn--Thr--OH conjugated with albumin or a modified albumin, collecting blood from the mammal, and concentrating said antibodies from said collected blood.
Claim 2
Dependent Claim
44 words
A process for the preparation of specific antibodies which comprises sensitizing a mammal by administering to said mammal a peptide of the formula H--Ser--Arg--Arg--Ala--Gln--Asp--Phe--Val--Gln--Trp--Leu--Met--Asn--Thr--OH conjugated with albumin or a modified albumin, collecting blood from the mammal, and concentrating said antibodies from said collected blood.
Claim 3
Dependent Claim
27 words
A process as claimed in claim 1, in which the peptide is conjugated with the use of glutaraldehyde or 3-(2'-benzothiazolyl-dithio) propionate succinimide ester as a conjugation reagent.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1986-12-26 | 1987-06-27 | 1987-12-27 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1990-12-26 | 1991-06-27 | 1991-12-27 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1994-12-26 | 1995-06-27 | 1995-12-27 | $7700.00 | Window Closed / Lapsed |