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US4419451A
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US
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Oxygen scavenging system for anaerobiosis
Assignee: Becton Dickinson and Co
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Filing: 1981-05-21
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Granted: 1983-12-06
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Expires: 2001-05-21
Abstract
Abstract A self-contained system is provided for producing an oxygen-free environment for anaerobiosis and culturing wherein the system possesses the ability to provide and maintain the oxygen-free environment, even after the opening and closing of the sealed container therefore several times. The system includes a metallic couple, preferably copper-magnesium, which reacts with water to induce and maintain the oxygen-free environment. The degree of addition of water to the system controls the rate of reduction of the environment. The system possesses a dual oxygen scavenging function by the controlled addition of water. That is, if a small amount of moisture is added to the system couple of the invention, hydrogen is liberated, which causes a drying in the system simultaneously with a reaction of a portion of the couple to gradually scavenge oxygen from the environment, simultaneously with the liberation of hydrogen. If desired, in such an arrangement, the subsequent addition of water generates hydrogen again until all the magnesium is used up, in order to provide a subsequent oxygen scavenging property. In addition, the system includes a sealable petri dish so conformed to include means for maintaining the system of the invention in combination with an indicator for anaerobiosis, a catalyst for the system, a carbon dioxide generator, and space for the culture media.
Inventors
- 👤 Richard L. Garner
- 👤 Luther Winans, Jr.
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-27 12:12
CPC Classifications
C; C12; C12M; C12M23/00; C12M23/02; C12M23/10; C12M41/00; C12M41/30; C12M41/34; Y; Y10; Y10S; Y10S435/00; Y10S435/801
Claims Analysis
Total Claims: 27
Claim 1
Independent Claim
107 words
A system for producing an atmosphere for use in culturing anaerobic microorganisms, characterized by (a) a sealable container; (b) a material in said container which when contacted by water generates a gas capable of reacting with oxygen to provide an atmosphere for culturing anaerobic microorganisms; (c) said material being a metal-metal salt couple wherein said metal is selected from the group consisting of magnesium, beryllium, barium, strontium, and calcium and wherein said metal salt is selected from the group consisting of copper, platinum, palladium, tin, iron, nickel and cobalt; and (d) catalytic means in said container for catalyzing reaction between oxygen and gas generated in said container.
Claim 2
Independent Claim
107 words
A system for producing an atmosphere for use in culturing anaerobic microorganisms, characterized by (a) a sealable container; (b) a material in said container which when contacted by water generates a gas capable of reacting with oxygen to provide an atmosphere for culturing anaerobic microorganisms; (c) said material being a metal-metal salt couple wherein said metal is selected from the group consisting of magnesium, beryllium, barium, strontium, and calcium and wherein said metal salt is selected from the group consisting of copper, platinum, palladium, tin, iron, nickel and cobalt; and (d) catalytic means in said container for catalyzing reaction between oxygen and gas generated in said container.
Claim 3
Dependent Claim
17 words
The system of claim 1, further characterized by (a) said metal-metal salt couple includes said catalytic means.
Claim 4
Dependent Claim
33 words
The system of claim 2, further characterized by (a) said metal is magnesium metal; and (b) said metal salt is a member selected from the group consisting of palladium, nickel, platinum and cobalt.
Claim 5
Dependent Claim
22 words
The system of claim 1, further characterized by (a) said metal is magnesium; and (b) said metal salt is a copper salt.
Claim 6
Dependent Claim
46 words
The system of claim 1, further characterized by said sealable container comprising (a) a bottom portion; (b) a plurality of wells in said bottom portion for receiving reactants in said system; (c) a top portion; and (d) cooperating locking means on said top and bottom portions.
Claim 7
Dependent Claim
48 words
The system of claim 5, further characterized by (a) one-half of said bottom portion is solid; (b) said plurality of wells formed is in said solid one half; and (c) the remaining half of said bottom portion opposite said solid half for receiving a media to be cultured.
Claim 8
Dependent Claim
21 words
The system of claim 5, further characterized by (a) said catalytic means painted onto the internal surface of said top portion.
Claim 9
Dependent Claim
17 words
The system of claim 7, further characterized by (a) said catalytic means is palladium supported on alumina.
Claim 10
Dependent Claim
15 words
The system of claim 1, further characterized by (a) a tablet for generating carbon dioxide.
Claim 11
Dependent Claim
25 words
The system of claim 9, further characterized by (a) said tablet is comprised of a water soluble solid carbonate and a water soluble solid acid.
Claim 12
Dependent Claim
13 words
The system of claim 9, further characterized by (a) a carbon dioxide indicator.
Claim 13
Dependent Claim
17 words
The system of claim 1, further characterized by (a) at least one anaerobic indicator in said container.
Claim 14
Independent Claim
98 words
A system for use in generating an anaerobic atmosphere in a sealable container, characterized by (a) a material which when contacted by water generates a gas capable of reacting with oxygen to provide an atmosphere for culturing anaerobic microorganisms; (b) said material being a metal-metal salt couple wherein said metal is selected from the group consisting of magnesium, beryllium, barium, strontium, and calcium and wherein said metal salt is selected from the group consisting of copper, platinum, palladium, tin, iron, nickel and cobalt; and (c) catalytic means for catalyzing reaction between oxygen and gas generated by said material.
Claim 15
Independent Claim
98 words
A system for use in generating an anaerobic atmosphere in a sealable container, characterized by (a) a material which when contacted by water generates a gas capable of reacting with oxygen to provide an atmosphere for culturing anaerobic microorganisms; (b) said material being a metal-metal salt couple wherein said metal is selected from the group consisting of magnesium, beryllium, barium, strontium, and calcium and wherein said metal salt is selected from the group consisting of copper, platinum, palladium, tin, iron, nickel and cobalt; and (c) catalytic means for catalyzing reaction between oxygen and gas generated by said material.
Claim 16
Dependent Claim
15 words
The system of claim 13, further characterized by (a) said material is a magnesium-copper couple.
Claim 17
Dependent Claim
16 words
The system of claim 14, further characterized by (a) said catalytic means is a supported palladium.
Claim 18
Dependent Claim
15 words
The system of claim 14, further characterized by (a) said material is a magnesium-palladium couple.
Claim 19
Dependent Claim
18 words
The system of claim 13, further characterized by (a) said material is in the form of a tablet.
Claim 20
Dependent Claim
17 words
The system of claim 17, further characterized by (a) a second tablet capable of generating carbon dioxide.
Claim 21
Dependent Claim
26 words
The system of claim 18, further characterized by (a) said second tablet is comprised of a water soluble solid carbonate and a water soluble solid acid.
Claim 22
Independent Claim
139 words
A system for producing an atmosphere for use in culturing anaerobic microorganisms, characterized by (a) a sealable petri dish; (b) a material in said petri dish which when contacted by water generates a gas capable of reacting with oxygen to provide an atmosphere for culturing anaerobic microorganisms; (c) said material being a metal-metal salt couple in the form of a tablet wherein said metal is selected from the group consisting of magnesium, beryllium, barium, strontium, and calcium and wherein said metal salt is selected from the group consisting of copper, platinum, palladium, tin, iron, nickel and cobalt; and (d) catalytic means in said container for catalyzing reaction between oxygen therein and gas generated in said container; (e) an anaerobic indicator in said petri dish; and (f) means on said petri dish for opening and resealing said petri dish repeatedly.
Claim 23
Independent Claim
139 words
A system for producing an atmosphere for use in culturing anaerobic microorganisms, characterized by (a) a sealable petri dish; (b) a material in said petri dish which when contacted by water generates a gas capable of reacting with oxygen to provide an atmosphere for culturing anaerobic microorganisms; (c) said material being a metal-metal salt couple in the form of a tablet wherein said metal is selected from the group consisting of magnesium, beryllium, barium, strontium, and calcium and wherein said metal salt is selected from the group consisting of copper, platinum, palladium, tin, iron, nickel and cobalt; and (d) catalytic means in said container for catalyzing reaction between oxygen therein and gas generated in said container; (e) an anaerobic indicator in said petri dish; and (f) means on said petri dish for opening and resealing said petri dish repeatedly.
Claim 24
Dependent Claim
37 words
The system of claim 20, further characterized by (a) said petri dish includes a cooperating top and bottom portion; and (b) said opening and resealing means includes cooperating bayonet locking means on said top and bottom portions.
Claim 25
Dependent Claim
25 words
The system of claim 20, further characterized by (a) said metal-metal salt couple is the product of the reaction between metallic magnesium and cupric chloride.
Claim 26
Dependent Claim
18 words
The system of claim 22, further characterized by (a) said tablet includes a tris buffer and sodium chloride.
Claim 27
Dependent Claim
27 words
The system of claim 20, further characterized by (a) said anaerobic indicator includes a member selected from the group consisting of methylene blue, indigo carmine and resazurin.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1986-12-05 | 1987-06-06 | 1987-12-06 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1990-12-05 | 1991-06-06 | 1991-12-06 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1994-12-05 | 1995-06-06 | 1995-12-06 | $7700.00 | Window Closed / Lapsed |