US4418145A
Expired / Lapsed
US
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Xanthomonas campestris ATCC 31601 and process for use
Assignee: BP Corp North America Inc
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Filing: 1980-07-14
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Granted: 1983-11-29
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Expires: 2000-07-14
Abstract
Abstract A new degenerative-resistant strain of Xanthomonas campestris is described which is suitable for long-term continuous fermentation to produce xanthan gum from a nutrient medium, typically containing inorganic salts, glucose, NH 4 Cl, and citric acid, with or without yeast extract or yeast autolysate.
Inventors
- 👤 William P. Weisrock
- 👤 Edward F. McCarthy
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-24 16:36
CPC Classifications
C; C12; C12N; C12N1/00; C12N1/20; C12N1/205; C12P; C12P19/00; C12P19/04; C12P19/06; C12R; C12R2001/00; C12R2001/01; C12R2001/64; Y; Y10; Y10S; Y10S435/00; Y10S435/8215; Y10S435/822; Y10S435/91
Claims Analysis
Total Claims: 22
Claim 1
Independent Claim
66 words
A method for the production of a heteropolysaccharide which comprises continuously culturing a degenerative resistant strain of bacteria designated Xanthomonas campestris XCP-19, having the identifying characteristics of ATCC 31601, in an aqueous nutrient comprising essentially assimilable sources of carbon, nitrogen and inorganic substances wherein said medium is continuously fed to a fermentation zone to produce said polysaccharide, and withdrawing the resulting fermented medium from said zone.
Claim 2
Independent Claim
66 words
A method for the production of a heteropolysaccharide which comprises continuously culturing a degenerative resistant strain of bacteria designated Xanthomonas campestris XCP-19, having the identifying characteristics of ATCC 31601, in an aqueous nutrient comprising essentially assimilable sources of carbon, nitrogen and inorganic substances wherein said medium is continuously fed to a fermentation zone to produce said polysaccharide, and withdrawing the resulting fermented medium from said zone.
Claim 3
Dependent Claim
13 words
The method of claim 1 in which said medium is a minimal medium.
Claim 4
Dependent Claim
27 words
The method of claim 2 wherein said minimal medium also contains as a complex nitrogen source, one of the group consisting of yeast extract and yeast autolysate.
Claim 5
Dependent Claim
23 words
The method of claim 1 in which the fermentation is conducted at a specific xanthan productivity in excess of 0.2 gm xanthan/gm cells/hour.
Claim 6
Dependent Claim
29 words
The method of claim 1 wherein said fermented medium is withdrawn from said zone at a rate such that an essentially steady state condition is maintained in said zone.
Claim 7
Independent Claim
95 words
A method for the production of a heteropolysaccharide which comprises continuously culturing a degenerative resistant strain of Xanthomonas campestris XCP-19 having the identifying characteristics of ATCC 31601 in a minimal medium containing a growth limiting nutrient and wherein said medium is continuously fed to a first fermentation zone to produce additional amounts of said strain together with said heteropolysaccharide, thereafter transferring the effluent from said first zone to a second fermentation zone, and adding a fermentable sugar to said effluent in said second zone whereby the formation of heteropolysaccharide in said second zone is maximized.
Claim 8
Independent Claim
95 words
A method for the production of a heteropolysaccharide which comprises continuously culturing a degenerative resistant strain of Xanthomonas campestris XCP-19 having the identifying characteristics of ATCC 31601 in a minimal medium containing a growth limiting nutrient and wherein said medium is continuously fed to a first fermentation zone to produce additional amounts of said strain together with said heteropolysaccharide, thereafter transferring the effluent from said first zone to a second fermentation zone, and adding a fermentable sugar to said effluent in said second zone whereby the formation of heteropolysaccharide in said second zone is maximized.
Claim 9
Dependent Claim
13 words
The method of claim 6 in which the fermentable sugar employed is glucose.
Claim 10
Independent Claim
13 words
The method of claims 1 or 6 in which the heteropolysaccharide is xanthan.
Claim 11
Dependent Claim
58 words
A biologically pure culture of a novel strain of Xanthomonas campestris XCP-19 having the identifying characteristics of ATCC 31601, said strain being capable of producing xanthan gum in recoverable amounts upon fermentation of an aqueous nutrient medium containing assimilable sources of carbon, nitrogen and inorganic substances and being resistant to cell degeneration when subjected to continuous fermentation conditions.
Claim 12
Dependent Claim
58 words
A biologically pure culture of a novel strain of Xanthomonas campestris XCP-19 having the identifying characteristics of ATCC 31601, said strain being capable of producing xanthan gum in recoverable amounts upon fermentation of an aqueous nutrient medium containing assimilable sources of carbon, nitrogen and inorganic substances and being resistant to cell degeneration when subjected to continuous fermentation conditions.
Claim 13
Dependent Claim
12 words
A biologically pure culture consisting essentially of Xanthomonas campestris XCP-19 ATCC 31601.
Claim 14
Dependent Claim
12 words
A biologically pure culture consisting essentially of Xanthomonas campestris XCP-19 ATCC 31601.
Claim 15
Dependent Claim
34 words
A biologically pure culture consisting essentially of Xanthomonas campestris XCP-19 ATCC 31601, said culture being degenerative resistant when continuously cultured in an aqueous nutrient medium, and capable of producing xanthan gum in good yields.
Claim 16
Dependent Claim
34 words
A biologically pure culture consisting essentially of Xanthomonas campestris XCP-19 ATCC 31601, said culture being degenerative resistant when continuously cultured in an aqueous nutrient medium, and capable of producing xanthan gum in good yields.
Claim 17
Dependent Claim
11 words
A bacterial culture consisting essentially of Xanthomonas campestris XCP-19 ATCC 31601.
Claim 18
Dependent Claim
11 words
A bacterial culture consisting essentially of Xanthomonas campestris XCP-19 ATCC 31601.
Claim 19
Dependent Claim
33 words
A bacterial culture consisting essentially of Xanthomonas campestris XCP-19 ATCC 31601, said culture being degenerative resistant when continuously cultured in an aqueous nutrient medium, and capable of producing xanthan gum in good yields.
Claim 20
Dependent Claim
33 words
A bacterial culture consisting essentially of Xanthomonas campestris XCP-19 ATCC 31601, said culture being degenerative resistant when continuously cultured in an aqueous nutrient medium, and capable of producing xanthan gum in good yields.
Claim 21
Dependent Claim
37 words
A bacterial culture consisting essentially of Xanthomonas campestris XCP-19 ATCC 31601, said culture capable of producing xanthan gum in recoverable amounts upon fermentation of an aqueous nutrient medium containing assimilable sources of carbon, nitrogen, and inorganic substances.
Claim 22
Dependent Claim
37 words
A bacterial culture consisting essentially of Xanthomonas campestris XCP-19 ATCC 31601, said culture capable of producing xanthan gum in recoverable amounts upon fermentation of an aqueous nutrient medium containing assimilable sources of carbon, nitrogen, and inorganic substances.
References Cited (Backward Citations)
US3773752A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1986-11-28 | 1987-05-30 | 1987-11-29 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1990-11-28 | 1991-05-30 | 1991-11-29 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1994-11-28 | 1995-05-30 | 1995-11-29 | $7700.00 | Window Closed / Lapsed |