US4417861A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Cell culture pumping system
Assignee: Monsanto Co
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Filing: 1981-12-24
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Granted: 1983-11-29
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Expires: 2001-12-24
Abstract
Abstract A low trauma, reversible flow pumping system is disclosed which is useful for transfer of biological fluids containing fragile components such as cells. The pumping system comprises a length of collapsible and flexible tubing in fluid communication at each end with a two-way, gravity actuated check valve means having a self-centering, vertically slidable weight member for directional regulation of fluid flow. Said tubing is sealingly enclosed within a hydraulic fluid containing chamber which is in fluid communication with oscillatory pressure providing means to cause alternate expansion and contraction of said tubing.
Inventors
- 👤 William R. Tolbert
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-05 14:57
CPC Classifications
F; F04; F04B; F04B43/00; F04B43/0009; F04B43/0054; F04B43/0072; Y; Y10; Y10T; Y10T137/00; Y10T137/5109; Y10T137/5153; Y10T137/7722; Y10T137/7837; Y10T137/7866; Y10T137/7867; Y10T137/7904; Y10T137/7908; Y10T137/7921
Claims Analysis
Total Claims: 7
Claim 1
Independent Claim
111 words
A low trauma, reversible flow pumping system for transfer of biological fluids containing fragile components which comprises a length of collapsible and flexible tubing having inlet and outlet means at opposite ends, each said end being in fluid communication with a two-way, gravity actuated check valve means having disposed therein a self-centering, vertically slidable weight member with a center of gravity below its sealing position and adapted to permit fluid to be pumped through said tubing in either direction, said tubing being sealing enclosed within a hydraulic fluid containing chamber and said chamber being in fluid communication with oscillatory pressure providing means to provide alternate expansion and collapsing of said tubing.
Claim 2
Dependent Claim
111 words
A low trauma, reversible flow pumping system for transfer of biological fluids containing fragile components which comprises a length of collapsible and flexible tubing having inlet and outlet means at opposite ends, each said end being in fluid communication with a two-way, gravity actuated check valve means having disposed therein a self-centering, vertically slidable weight member with a center of gravity below its sealing position and adapted to permit fluid to be pumped through said tubing in either direction, said tubing being sealing enclosed within a hydraulic fluid containing chamber and said chamber being in fluid communication with oscillatory pressure providing means to provide alternate expansion and collapsing of said tubing.
Claim 3
Dependent Claim
102 words
The pumping system of claim 1 in which said check valve means comprises an outer housing with inlet and outlet ports, an inner double-ended, cylindrical seal assembly concentric with said housing and having seating means at its opposite ends, a self-centering, centrally disposed elongated slidable weight member adapted to vertically penetrate the bore of said seal assembly, said weight member having a length greater than the length of said seal assembly, conically tapered opposite ends adapted for leak-proof seating on said seal assembly seating means and a center of gravity at about a narrowed shank portion intermediate said conically tapered opposite ends.
Claim 4
Dependent Claim
26 words
The pumping system of claim 2 including a spacer ring and an annular weight positioned on each side of said cylindrical seal assembly and concentric therewith.
Claim 5
Dependent Claim
15 words
The pumping system of claim 1 in which said flexible tubing is impervious to liquids.
Claim 6
Independent Claim
125 words
The method of transferring a cell culture suspension between interconnected vessels comprising pumping said suspension through a low trauma, reversible flow pumping system positioned intermediate said vessels, said pumping system comprising a length of collapsible and flexible tubing having inlet and outlet means at opposite ends, each said end being in fluid communication with a two-way, gravity actuated check valve means having disposed therein a self-centering, vertically slidable weight member with a center of gravity below its sealing position and adapted to permit fluid to be pumped through said tubing in either direction, said tubing being sealingly enclosed within a hydraulic fluid containing chamber and said chamber being in fluid communication with oscillatory pressure providing means to provide alternate expansion and collapsing of said tubing.
Claim 7
Independent Claim
125 words
The method of transferring a cell culture suspension between interconnected vessels comprising pumping said suspension through a low trauma, reversible flow pumping system positioned intermediate said vessels, said pumping system comprising a length of collapsible and flexible tubing having inlet and outlet means at opposite ends, each said end being in fluid communication with a two-way, gravity actuated check valve means having disposed therein a self-centering, vertically slidable weight member with a center of gravity below its sealing position and adapted to permit fluid to be pumped through said tubing in either direction, said tubing being sealingly enclosed within a hydraulic fluid containing chamber and said chamber being in fluid communication with oscillatory pressure providing means to provide alternate expansion and collapsing of said tubing.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1986-11-28 | 1987-05-30 | 1987-11-29 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1990-11-28 | 1991-05-30 | 1991-11-29 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1994-11-28 | 1995-05-30 | 1995-11-29 | $7700.00 | Window Closed / Lapsed |