US4416983A
Expired / Lapsed
US
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Determination of NAD(P)H or salicylate
Assignee: Roche Diagnostics GmbH
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Filing: 1981-12-07
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Granted: 1983-11-22
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Expires: 2001-12-07
Abstract
Abstract The present invention provides a process for the determination of NAD(P)H or of salicylate, wherein, in a NAD(P)H-dependent reaction, salicylate is decarboxylated by salicylate hydroxylase and a colored material is formed from the decarboxylation product in the presence of tyrosinase by oxidative coupling with an appropriate colored material component, the colored material formed then being determined photometrically. The present invention also provides a reagent for the determination of NADH or NADPH, wherein it contains salicylate, a chromogenic hydrazone or amine, salicylate hydroxylase, tyrosinase and buffer, as well as a reagent for the determination of salicylate, wherein it contains NAD(P)H, a chromogenic hydrazone or amine, salicylate hydroxylase, tyrosinase and buffer.
Inventors
- 👤 Albert Roder
- 👤 Joachim Siedel
- 👤 Hans Mollering
- 👤 Hans Seidel
- 👤 Helmgard Gauhl
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-29 07:31
CPC Classifications
C; C12; C12Q; C12Q1/00; C12Q1/26; C12Q1/008; Y; Y10; Y10S; Y10S435/00; Y10S435/805; Y10S435/81
Claims Analysis
Total Claims: 11
Claim 1
Independent Claim
68 words
Method for the determination of NAD(P)H or of salicylate comprising; (a) decarboxylating salicylate by use of salicylate hydroxylase in a NAD(P)H dependent reaction in the presence of tyrosinase and of an appropriate chromogenic component selected from the group consisting of hydrazones and amines to form a colored material by oxidative coupling of the decarboxylation product with the said chromogenic component; and (b) determining the colored material formed photometrically.
Claim 2
Dependent Claim
68 words
Method for the determination of NAD(P)H or of salicylate comprising; (a) decarboxylating salicylate by use of salicylate hydroxylase in a NAD(P)H dependent reaction in the presence of tyrosinase and of an appropriate chromogenic component selected from the group consisting of hydrazones and amines to form a colored material by oxidative coupling of the decarboxylation product with the said chromogenic component; and (b) determining the colored material formed photometrically.
Claim 3
Dependent Claim
10 words
Method as claimed in claim 1, wherein NAD(P)H is determined.
Claim 4
Dependent Claim
10 words
Method as claimed in claim 1, wherein salicylate is determined.
Claim 5
Dependent Claim
19 words
Reagent for the determination of NADH comprising salicylate, a chromogenic hydrazone or amine, salicylate hydroxylase, tyrosinase and a buffer.
Claim 6
Dependent Claim
19 words
Reagent for the determination of NADH comprising salicylate, a chromogenic hydrazone or amine, salicylate hydroxylase, tyrosinase and a buffer.
Claim 7
Dependent Claim
18 words
Reagent for the determination of salicylate, comprising NAD(P)H, a chromogenic hydrazone or amine, salicylate hydroxylase, tyrosinase and buffer.
Claim 8
Dependent Claim
18 words
Reagent for the determination of salicylate, comprising NAD(P)H, a chromogenic hydrazone or amine, salicylate hydroxylase, tyrosinase and buffer.
Claim 9
Dependent Claim
36 words
Reagent as claimed in claim 4 comprising: 0.05 to 20 mmol/liter salicylate, 0.3 to 30 mmol/liter hydrazone or amine, 0.05 to 5 kU/liter salicylate hydroxylase, 10 to 200 kU/liter tyrosinase and buffer (pH 6.0 to 9.3).
Claim 10
Dependent Claim
36 words
Reagent as claimed in claim 5 comprising: 0.05 to 1.5 mmol/liter NAD(P)H, 0.3 to 30 mmol/liter hydrazone or amine, 0.05 to 5 kU/liter salicylate hydroxylase, 10 to 200 kU/liter tyrosinase and buffer (pH 6.0 to 9.3).
Claim 11
Dependent Claim
15 words
Reagent as claimed in claim 4, wherein the hydrazone is 3-methyl-benzothiazol-2-one hydrazone 6-sulfonic acid (MBTH-S).
References Cited (Backward Citations)
US4168205A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1986-11-21 | 1987-05-23 | 1987-11-22 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1990-11-21 | 1991-05-23 | 1991-11-22 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1994-11-21 | 1995-05-23 | 1995-11-22 | $7700.00 | Window Closed / Lapsed |