US4415663A
Expired / Lapsed
US
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Support matrix for immobilized enzymes
Assignee: Honeywell UOP LLC
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Filing: 1982-08-25
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Granted: 1983-11-15
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Expires: 2002-08-25
Abstract
Abstract A support matrix comprising a porous support impregnated with a polyamine substantially all of whose nitrogens bear pendant epoxide groups can be readily used to immobilize enzymes. Because immobilization results from formation of a hydrolytically stable carbon-nitrogen single bond, the resulting immobilized enzyme system may be advantageously used where hydrolytic instability of the bound enzyme is an important consideration.
Inventors
- 👤 Ted Symon
- 👤 Chester F. Barszcz
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-03 08:54
CPC Classifications
C; C12; C12N; C12N11/00; C12N11/14; C12N11/02; C12N11/08; C12N11/089; C12N11/091; Y; Y10; Y10T; Y10T428/00; Y10T428/31504; Y10T428/31551; Y10T428/31645; Y10T428/31652; Y10T428/31667
Claims Analysis
Total Claims: 17
Claim 1
Independent Claim
66 words
A support matrix comprising a porous support selected from the group consisting of alumina, silica, thoria, magnesia, porous glass, ceramics, and combinations thereof, impregnated with a polyamine selected from the group consisting of poly(ethyleneamines) and epiamines, substantially all of whose nitrogens bear a pendant epoxide group, wherein said epiamines are the reaction products of polyepichlorohydrin with an alkylenediamine containing from 2 to about 10 carbon atoms.
Claim 2
Dependent Claim
66 words
A support matrix comprising a porous support selected from the group consisting of alumina, silica, thoria, magnesia, porous glass, ceramics, and combinations thereof, impregnated with a polyamine selected from the group consisting of poly(ethyleneamines) and epiamines, substantially all of whose nitrogens bear a pendant epoxide group, wherein said epiamines are the reaction products of polyepichlorohydrin with an alkylenediamine containing from 2 to about 10 carbon atoms.
Claim 3
Dependent Claim
11 words
The support matrix of claim 1 where the support is alumina.
Claim 4
Dependent Claim
22 words
The support matrix of claim 1 where the polyamine is selected from the group consisting of diethylenetriamine, triethylenetetramine, tetraethylenepentamine, pentamethylenehexamine, and polyethyleneimine.
Claim 5
Dependent Claim
11 words
The support matrix of claim 3 where the polyamine is polyethyleneimine.
Claim 6
Dependent Claim
20 words
The support matrix of claim 1 where the alkylenediamine is selected from the group consisting of ethylenediamine, 1,2-diaminopropane, and 1,3-diaminopropane.
Claim 7
Dependent Claim
20 words
The support matrix of claim 1 where the group pendant to the nitrogen of the polyamine is a 2,3-epoxypropyl group.
Claim 8
Independent Claim
114 words
A method of making a support matrix comprising impregnating a porous support selected from the group consisting of alumina, silica, thoria, magnesia, porous glass, ceramics, and combinations thereof with a polyamine selected from the group consisting of poly(ethyleneamines) and epiamines wherein said epiamines are the reaction products of polyepichlorohydrin with an alkylenediamine containing from 2 to about 10 carbon atoms, removing excess polyamine, treating the impregnated support with an excess of an epihalohydrin so as to react substantially all of the nitrogens of the polyamine with the epihalohydrin, removing excess unreacted epihalohydrin, contacting the resulting material with base so as to regenerate an epoxide moiety, removing excess base, and recovering the resulting support matrix.
Claim 9
Independent Claim
114 words
A method of making a support matrix comprising impregnating a porous support selected from the group consisting of alumina, silica, thoria, magnesia, porous glass, ceramics, and combinations thereof with a polyamine selected from the group consisting of poly(ethyleneamines) and epiamines wherein said epiamines are the reaction products of polyepichlorohydrin with an alkylenediamine containing from 2 to about 10 carbon atoms, removing excess polyamine, treating the impregnated support with an excess of an epihalohydrin so as to react substantially all of the nitrogens of the polyamine with the epihalohydrin, removing excess unreacted epihalohydrin, contacting the resulting material with base so as to regenerate an epoxide moiety, removing excess base, and recovering the resulting support matrix.
Claim 10
Dependent Claim
10 words
The method of claim 7 where the support is alumina.
Claim 11
Dependent Claim
21 words
The method of claim 7 where the polyamine is selected from the group consisting of diethylenetriamine, triethylenetetramine, tetraethylenepentamine, pentamethylenehexamine, and polyethyleneimine.
Claim 12
Dependent Claim
10 words
The method of claim 9 where the polyamine is polyethyleneimine.
Claim 13
Dependent Claim
17 words
The method of claim 7 where the epihalohydrin contains from about 3 to about 6 carbon atoms.
Claim 14
Dependent Claim
11 words
The method of claim 11 where the epihalohydrin is a 3-halo-1,2-epoxypropane.
Claim 15
Dependent Claim
10 words
The method of claim 12 where the epihalohydrin is 3-chloro-1,2-epoxypropane.
Claim 16
Dependent Claim
17 words
An immobilized enzyme system comprising the support matrix of claim 1 having an enzyme covalently bonded thereto.
Claim 17
Dependent Claim
45 words
The immobilized enzyme system of claim 14 where the enzyme is selected from the group consisting of glucose isomerase, glucoamylase, cholesteroloxidase, alcohol dehydrogenase, amino acid oxidase, arginase, asparaginase, catalase, chymotrypsin, cellulase, collagenase, deoxyribonuclease, ficin, histidase, lactase, peroxidase, lysozyme, gamma amylase, papain, rennin, ribonuclease and urease.
References Cited (Backward Citations)
US4141857A
Cited
SU755296A1
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1986-11-14 | 1987-05-16 | 1987-11-15 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1990-11-14 | 1991-05-16 | 1991-11-15 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1994-11-14 | 1995-05-16 | 1995-11-15 | $7700.00 | Window Closed / Lapsed |