Protective solution for heart and kidney and process for its preparation

Claim 2

In a method for preventing ischemia damage to the heart, kidneys and other organs during operations and transplants thereof, wherein an ischemia damage preventing effective amount of a solution containing a buffer system composed of histidine; histidine chloride, and tryptophane and the conventional electrolytes for cardioplegic solutions is administered, the improvement which comprises said solution also containing an ischemia damage protecting amount of α-ketoglutarate.

Claim 3

The method of claim 1 wherein the solution further contains potassium and magnesium ions as electrolytes and a polyol or a sugar.

Claim 4

A process for the preparation of a solution for preventing ischemia damage to the heart and kidneys during operations and while transplanting the organs, comprising adding to water on a per liter basis: ______________________________________ 4 ± 3 millimoles potassium or sodium hydrogen α-ketoglutarate 15 ± 8 millimoles of sodium chloride 10 ± 8 millimoles of potassium chloride 10 ± 2 millimoles of magnesium chloride 2 ± 1 millimole of tryptophane 150 ± 100 millimoles of histidine 16 ± 11 millimoles of histidine hydrochloride; ______________________________________ at least one polyol or sugar, and wherein the osmolarity of the solution is maintained at about 300 to 350 mosm and the pH of the solution is adjusted to 6.8 to 7.4.

Claim 5

A process for the preparation of a solution for preventing ischemia damage to the heart and kidneys during operations and while transplanting the organs, comprising adding to water on a per liter basis: ______________________________________ 4 ± 3 millimoles potassium or sodium hydrogen α-ketoglutarate 15 ± 8 millimoles of sodium chloride 10 ± 8 millimoles of potassium chloride 10 ± 2 millimoles of magnesium chloride 2 ± 1 millimole of tryptophane 150 ± 100 millimoles of histidine 16 ± 11 millimoles of histidine hydrochloride; ______________________________________ at least one polyol or sugar, and wherein the osmolarity of the solution is maintained at about 300 to 350 mosm and the pH of the solution is adjusted to 6.8 to 7.4.

Claim 6

In a method for preventing ischemia damage to the heart, kidneys and other organs during operations and transplants thereof, wherein an ischemia damage preventing effective amount of a solution is administered by perfusion, the improvement which comprises the solution having the following composition on a per liter basis: ______________________________________ potassium or sodium hydrogen 4 ± 3 millimoles α-ketoglutarate sodium chloride 15 ± 8 millimoles potassium chloride 10 ± 8 millimoles magnesium chloride 10 ± 2 millimoles tryptophane 2 ± 1 millimole histidine 150 ± 100 millimoles histidine hydrochloride 16 ± 11 millimoles mannitol 50 ± 50 millimoles fructose 50 ± 50 millimoles ribose 50 ± 50 millimoles inosine 50 ± 50 millimoles ______________________________________ the osmolarity of the solution being about 300 to 350 mosm and the pH of the solution lying between 6.8 and 7.4.

Claim 7

In a method for preventing ischemia damage to the heart, kidneys and other organs during operations and transplants thereof, wherein an ischemia damage preventing effective amount of a solution is administered by perfusion, the improvement which comprises the solution having the following composition on a per liter basis: ______________________________________ potassium or sodium hydrogen 4 ± 3 millimoles α-ketoglutarate sodium chloride 15 ± 8 millimoles potassium chloride 10 ± 8 millimoles magnesium chloride 10 ± 2 millimoles tryptophane 2 ± 1 millimole histidine 150 ± 100 millimoles histidine hydrochloride 16 ± 11 millimoles mannitol 50 ± 50 millimoles fructose 50 ± 50 millimoles ribose 50 ± 50 millimoles inosine 50 ± 50 millimoles ______________________________________ the osmolarity of the solution being about 300 to 350 mosm and the pH of the solution lying between 6.8 and 7.4.

Claim 8

In a protective solution for preventing ischemia damage to the heart and kidneys in operations and during organ transplants containing a buffer system composed of histidine, histidine hydrochloride, and tryptophane and the conventional electrolytes for cardioplegic solutions, the improvement which comprises said solution having the following composition on a per liter basis ______________________________________ potassium or sodium hydrogen 4 ± 3 millimoles α-ketoglutarate sodium chloride 15 ± 8 millimoles potassium chloride 10 ± 8 millimoles magnesium chloride 10 ± 2 millimoles tryptophane 2 ± 1 millimole histidine 150 ± 100 millimoles histidine hydrochloride 16 ± 11 millimoles mannitol 50 ± 50 millimoles fructose 50 ± 50 millimoles ribose 50 ± 50 millimoles inosine 50 ± 50 millimoles ______________________________________ the osmolarity of the solution being about 330 to 350 mosm and the pH of the solution lying between 6.8 and 7.4.

Claim 9

In a protective solution for preventing ischemia damage to the heart and kidneys in operations and during organ transplants containing a buffer system composed of histidine, histidine hydrochloride, and tryptophane and the conventional electrolytes for cardioplegic solutions, the improvement which comprises said solution having the following composition on a per liter basis ______________________________________ potassium or sodium hydrogen 4 ± 3 millimoles α-ketoglutarate sodium chloride 15 ± 8 millimoles potassium chloride 10 ± 8 millimoles magnesium chloride 10 ± 2 millimoles tryptophane 2 ± 1 millimole histidine 150 ± 100 millimoles histidine hydrochloride 16 ± 11 millimoles mannitol 50 ± 50 millimoles fructose 50 ± 50 millimoles ribose 50 ± 50 millimoles inosine 50 ± 50 millimoles ______________________________________ the osmolarity of the solution being about 330 to 350 mosm and the pH of the solution lying between 6.8 and 7.4.