US4415552A
Expired / Lapsed
US
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Composition for establishing immunological tolerance
Assignee: University of Alberta
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Filing: 1979-12-05
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Granted: 1983-11-15
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Expires: 1999-12-05
Abstract
Abstract A water-soluble non-immunogenic carrier, namely carboxymethyl cellulose, is provided which, in attachment to allergens or other haptens, is capable of inducing an immunological tolerance in an individual of a mammalian species to the allergen or hapten. The allergen or hapten is attached to each of a plurality of the carboxyl groups of carboxymethyl cellulose. Tolerance has been induced in mice to three particular haptens, namely 2,4-dinitrophenyl, penicillin G and fluorescein in the specific embodiments of the present invention.
Inventors
- 👤 Erwin Diener
- 👤 Uriel Diner
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-25 09:04
CPC Classifications
A; A61; A61K; A61K39/00; A61K39/35; A61K2039/60; A61K2039/6093; Y; Y10; Y10S; Y10S530/00; Y10S530/81; Y10S530/812; Y10S530/813; Y10S530/814
Claims Analysis
Total Claims: 18
Claim 1
Independent Claim
63 words
A pharmaceutically acceptable injectable composition consisting essentially of a water soluble conjugate of: a substantially non-cross-linked water soluble carboxymethyl cellulose carrier, and a plurality of allergen molecules, each chemically attached to a carboxyl group of the carboxymethyl cellulose carrier, said composition being capable upon injection in a mammal of inducing immunological tolerance to the allergen without evoking an allergenic response to the allergen.
Claim 2
Dependent Claim
63 words
A pharmaceutically acceptable injectable composition consisting essentially of a water soluble conjugate of: a substantially non-cross-linked water soluble carboxymethyl cellulose carrier, and a plurality of allergen molecules, each chemically attached to a carboxyl group of the carboxymethyl cellulose carrier, said composition being capable upon injection in a mammal of inducing immunological tolerance to the allergen without evoking an allergenic response to the allergen.
Claim 3
Dependent Claim
31 words
A composition as set forth in claim 1 wherein the carrier is a water soluble amino alkylated derivative of carboxymethyl cellulose and the allergen is attached to the amino group thereof.
Claim 4
Dependent Claim
18 words
The composition as set forth in claim 2 wherein the allergen is a derivative of the penicillin molecule.
Claim 5
Dependent Claim
15 words
The composition as set forth in claim 2 wherein the allergen is 2,4-dinitrophenyl or fluorescein.
Claim 6
Dependent Claim
13 words
The composition as set forth in claim 1 which is an aqueous solution.
Claim 7
Dependent Claim
13 words
The composition as set forth in claim 2 which is an aqueous solution.
Claim 8
Independent Claim
80 words
A method of converting an allergen molecule to a form immunologically acceptable for inducing an immunological tolerance in mammals to the allergen without evoking an allergenic response thereto, comprising: condensing each of a plurality of allergen molecules to a carboxyl group of a water soluble carboxymethyl cellulose carrier to form a water soluble carboxymethyl cellulose-allergen conjugate in which the carboxymethyl cellulose strands are substantially non-crosslinked; and recovering the resulting conjugate from the reaction mixture as a pharmaceutically acceptable injectable composition.
Claim 9
Independent Claim
80 words
A method of converting an allergen molecule to a form immunologically acceptable for inducing an immunological tolerance in mammals to the allergen without evoking an allergenic response thereto, comprising: condensing each of a plurality of allergen molecules to a carboxyl group of a water soluble carboxymethyl cellulose carrier to form a water soluble carboxymethyl cellulose-allergen conjugate in which the carboxymethyl cellulose strands are substantially non-crosslinked; and recovering the resulting conjugate from the reaction mixture as a pharmaceutically acceptable injectable composition.
Claim 10
Dependent Claim
30 words
The method as set forth in claim 7 wherein the carrier is an aminoalkylated derivative of carboxymethyl cellulose having free amino groups and the allergen is attached to said groups.
Claim 11
Dependent Claim
80 words
The method as set forth in claim 7, wherein the carrier is produced by: reacting a water soluble carboxymethyl cellulose with a diamine reagent at conditions such that one of the amino groups is inactive, to form as a carrier for the allergen a water soluble amino alkylated derivative of carboxymethyl cellulose, activating the previously inactive amino group; and condensing each of a plurality of allergen molecules to the free amino group to form a water soluble carboxymethyl cellulose-allergen conjugate.
Claim 12
Dependent Claim
68 words
A method as set forth in claim 9 comprising: reacting a water soluble carboxymethyl cellulose with an excess of ethylene diamine at a pH in the approximate range of 4.7 to 5.3 to form water soluble N-(2-aminoethyl)carbamylmethylated cellulose; and condensing at an alkaline pH, each of a plurality of allergen molecules to the free amino group of the N-(2-aminoethyl)carbamylmethylated cellulose to form a water soluble carboxymethyl cellulose-allergen conjugate.
Claim 13
Dependent Claim
18 words
The method as set forth in claim 10 wherein the allergen is a derivative of the penicillin molecule.
Claim 14
Dependent Claim
15 words
The method as set forth in claim 10 wherein the allergen is 2,4-dinitrophenyl or fluorescein.
Claim 15
Dependent Claim
37 words
The method for inducing immunological tolerance in a patient comprising intravenously or intraperitoneally administering to the patient an amount of a composition of claim 1 effective to induce immunological unresponsiveness to a subsequent challenge with the allergen.
Claim 16
Dependent Claim
47 words
A method as set forth in claim 13 wherein the conjugate of the composition administered to the patient is a conjugate of water soluble aminoalkyl derivative of carboxymethyl cellulose and a plurality of allergen molecules, each attached to a free amino group of the carboxymethyl cellulose derivative.
Claim 17
Dependent Claim
18 words
The method as set forth in claim 14 wherein the allergen is a derivative of the penicillin molecule.
Claim 18
Dependent Claim
15 words
The method as set forth in claim 14 wherein the allergen is 2,4-dinitrophenyl or fluorescein.
References Cited (Backward Citations)
US3794630A
Cited
US3959080A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1986-11-14 | 1987-05-16 | 1987-11-15 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1990-11-14 | 1991-05-16 | 1991-11-15 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1994-11-14 | 1995-05-16 | 1995-11-15 | $7700.00 | Window Closed / Lapsed |