Abstract An apparatus for intraoperative diagnosis is described which comprises a refrigerating device adapted to sprinkle a cryogenic liquid on the surface of a slide whereby the surface of the slide is cooled to at least -4° C. up to -8° C., at least one cooling fan which increases the evaporation of the cryogenic liquid and permits to achieve the necessary hypothermia on the slide; an automatic appositor which permits to exert a measurable and graduated pressure onto the section of the organ which has been interposed between the appositor and the slide at the time in which the section has reached a predetermined temperature. The appositor is provided with means for varying automatically the pressure during the advance of the slide. The refrigerating device comprises a cylinder containing a cryogenic liquid. The invention also resides in the method of carrying out histological analysis which consists of placing a section of tissue under examination on a slide, cooling vigorously the slide whereby the tissue undergoes a thermic shock and exfoliates on the slide. Pressure is applied after the section of tissue has been cooled.
An apparatus for preparing a section of an organ for intraoperative diagnosis which comprises a refrigerating device which sprinkles a cryogenic liquid on the surface of a slide whereby the surface of the slide is cooled to at least -4° C. up to -8° C., at least one cooling fan which increases the evaporation of the cryogenic liquid to achieve the necessary hypothermia on the slide; an appositor which exerts a measurable and graduated pressure onto the section of the organ which has been interposed between said appositor and the slide at the time in which said section has reached a predetermined temperature.
Claim 2
Independent Claim
103 words
An apparatus for preparing a section of an organ for intraoperative diagnosis which comprises a refrigerating device which sprinkles a cryogenic liquid on the surface of a slide whereby the surface of the slide is cooled to at least -4° C. up to -8° C., at least one cooling fan which increases the evaporation of the cryogenic liquid to achieve the necessary hypothermia on the slide; an appositor which exerts a measurable and graduated pressure onto the section of the organ which has been interposed between said appositor and the slide at the time in which said section has reached a predetermined temperature.
Claim 3
Dependent Claim
17 words
The apparatus according to claim 1 wherein the refrigerating device comprises a cylinder containing a cryogenic liquid.
Claim 4
Dependent Claim
21 words
The apparatus according to claim 2 which comprises means for increasing the rate of exit of the liquid from the cylinder.
Claim 5
Dependent Claim
19 words
The apparatus according to claim 3 wherein said means are a pressurized gas above the liquid in said cylinder.
Claim 6
Dependent Claim
31 words
The apparatus according to claim 1 wherein the refrigerating device comprises a nozzle capable of sprinkling the cryogenic liquid and capable of being oriented onto the central portion of the slide.
Claim 7
Dependent Claim
21 words
The apparatus according to claim 1 wherein the cryogenic liquid is ethyl chloride or other liquid having a low boiling point.
Claim 8
Dependent Claim
15 words
The apparatus according to claim 1 wherein the cryogenic liquid consists of a liquefied gas.
Claim 9
Dependent Claim
12 words
The apparatus according to claim 5 wherein the liquefied gas is nitrogen.