US4412973A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Autonomous simultaneous analysis apparatus and a method of using it
Assignee: Individual
•
Filing: 1981-12-14
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Granted: 1983-11-01
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Expires: 2001-12-14
Abstract
Abstract Analysis apparatus which uses a solid reaction support designed to hold successively a quantity of a compound such as a biological liquid which contains a substance to be analyzed, and then a quantity of a first reagent on which is fixed a biological indicator. The apparatus is constituted by an analysis rotor (1) which includes a plurality of peripheral reaction cells (2) each of which contains said reaction support (3), said cells being provided with a peripheral liquid-removal orifice (7). Means (4, 5) convey a washing liquid towards each reaction cell. Each of said reaction cells (2) is associated with a buffer cell (10), which successively receives at least the compound and the first reagent. It shares a common wall (11) with the reaction cell (2). A passage (25) is provided between the cells of each pair through said common wall (11). The first reagent is contained in a suitable receptacle (14) supported by the analysis apparatus. Means are provided to open said receptacle (14) to pour the reagent into the buffer cell (10).
Inventors
- 👤 Jean Guigan
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-04 05:10
CPC Classifications
G; G01; G01N; G01N21/00; G01N21/01; G01N21/03; G01N21/07; B; B04; B04B; B04B5/00; B04B5/04; B04B5/0407
Claims Analysis
Total Claims: 15
Claim 1
Independent Claim
174 words
Analysis apparatus of the kind which uses a reaction support to hold successively a quantity of a liquid which contains a substance to be analysed and then a quantity of a liquid reagent to react therewith, said apparatus comprising an analysis rotor having: a plurality of peripheral reaction cells each containing a solid reaction support; means for centrifuging a washing liquid directly into each reaction cell; a peripheral liquid-removal orifice for each reaction cell; the improvement comprising: a buffer cell operatively associated with each reaction cell; separate means for successively centrifuging at least said liquid containing a compound to be analysed and a first reagent directly into said buffer cell; means for storing predetermined quantities of said first reagent in sealed receptacles and for opening said receptacles when it is required to centrifuge the first reagent into said buffer cells; means for communicating said reaction cells and said buffer cells; and the buffer cell-reaction cell pairs being so arranged that liquid in the buffer cells moves into the associated reaction cells when centrifuging stops.
Claim 2
Dependent Claim
174 words
Analysis apparatus of the kind which uses a reaction support to hold successively a quantity of a liquid which contains a substance to be analysed and then a quantity of a liquid reagent to react therewith, said apparatus comprising an analysis rotor having: a plurality of peripheral reaction cells each containing a solid reaction support; means for centrifuging a washing liquid directly into each reaction cell; a peripheral liquid-removal orifice for each reaction cell; the improvement comprising: a buffer cell operatively associated with each reaction cell; separate means for successively centrifuging at least said liquid containing a compound to be analysed and a first reagent directly into said buffer cell; means for storing predetermined quantities of said first reagent in sealed receptacles and for opening said receptacles when it is required to centrifuge the first reagent into said buffer cells; means for communicating said reaction cells and said buffer cells; and the buffer cell-reaction cell pairs being so arranged that liquid in the buffer cells moves into the associated reaction cells when centrifuging stops.
Claim 3
Dependent Claim
25 words
Analysis apparatus according to claim 1, wherein the cells of each pair are juxtaposed, with a common radial wall separating the cells from each other.
Claim 4
Dependent Claim
33 words
Analysis apparatus according to claim 1, wherein the cells of each pair are superposed, with a common horizontal wall comprising the upper surface of the reaction cell separating the cells from each other.
Claim 5
Dependent Claim
57 words
Analysis apparatus according to claim 1, wherein the internal profile of the buffer cells is such as to ensure that any liquid brought into said cell during centrifuging is unable to escape towards the reaction cell until centrifuging is stopped, and where upon all the liquid brought into the buffer cell passes into the associated reaction cell.
Claim 6
Dependent Claim
36 words
Analysis apparatus according to claim 1, wherein the means by which the washing liquid is conveyed comprise a central inlet orifice and radial pipes connecting said central orifice to each of the peripheral reaction cells, respectively.
Claim 7
Dependent Claim
43 words
Apparatus according to claim 5, wherein the liquid which contains the substance to be analyzed is applied to the rotor via an orifice which is concentric with the inlet orifice for the washing liquid, radial pipes connecting said orifice to the buffer cells.
Claim 8
Dependent Claim
49 words
Apparatus according to claim 6, wherein the end of each radial pipe which leads to the buffer cells has a capillary constriction preceded by a pocket, said constriction together with a shutter insertable into the concentric orifice to separate it from said pockets serving to delimit a predetermined volume.
Claim 9
Dependent Claim
17 words
Analysis apparatus according to claim 1, wherein the reaction support is formed by at least one bead.
Claim 10
Dependent Claim
42 words
Analysis apparatus according to claim 8, wherein the support is constituted by a plurality of beads of very small dimensions, and a barrier is disposed in each reaction cell adjacent said peripheral liquid-removal orifice and said barrier retaining the beads during centrifuging.
Claim 11
Dependent Claim
15 words
Analysis apparatus according to claim 9, wherein said barrier is of the molecular filter type.
Claim 12
Dependent Claim
20 words
Apparatus according to claim 9 or 10, wherein the diameter of said beads lies between substantially 10 and 20 microns.
Claim 13
Dependent Claim
26 words
Apparatus according to claim 1, wherein the reaction support is constituted by a coating deposited on the lower portion of the wall of each reaction cell.
Claim 14
Dependent Claim
53 words
Analysis apparatus according to claim 1, wherein the inner and outer surfaces of the reaction cells are substantially parallel at least at the level of an observation window, the outer surface further having a side spout ending in said liquid removal orifice and allowing the complete removal of the washing liquid during centrifuging.
Claim 15
Dependent Claim
29 words
Analysis apparatus according to claim 13, wherein the spout is located at the rear of the reaction cell relative to the direction of rotation used during the washing operation.
References Cited (Backward Citations)
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1986-10-31 | 1987-05-02 | 1987-11-01 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1990-10-31 | 1991-05-02 | 1991-11-01 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1994-10-31 | 1995-05-02 | 1995-11-01 | $7700.00 | Window Closed / Lapsed |