US4412951A
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US
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Reagents for measuring lipase activity
Assignee: Fujifilm Holdings Corp
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Filing: 1981-10-28
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Granted: 1983-11-01
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Expires: 2001-10-28
Abstract
Abstract A reagent for measuring a lipase activity comprised of a higher fatty acid ester having the general formula ##STR1## wherein R 1 is a straight chain alkyl group; R 2 is hydrogen or a halogen atom; R 3 and R 4 are independently a nitro group, an alkylsulfonyl group, a substituted alkylsulfonyl group or a trifluoromethyl group; G is an --SO 3 M group or a group having at least one --SO 3 M where M represents sodium or potassium, or, when R 4 has a sulfonic acid group, may be a hydrogen atom; and Q is hydrogen, a halogen atom, an alkyl group, an alkoxy-substituted alkyl group, an alkoxy group, an alkoxy-substituted alkoxy group, a sulfonamido group, a sulfamoyl group, a carbonamido group, a carbamoyl group, or an alkylsulfonyl group. The reagent is capable of releasing a dye on enzymatic hydrolysis by the action of lipase. Because of the released dye has a light absorption zone within the longer wavelength region, it is not substantially disturbed by colored matter contained within blood making it possible to obtain excellent operability and reproducibility in the measurement of lipase activity within blood.
Inventors
- 👤 Yoshio Inagaki
- 👤 Masaki Okazaki
- 👤 Shinsaku Fujita
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-09 18:11
CPC Classifications
C; C09; C09B; C09B43/00; C09B43/18; C09B43/20; C09B43/202; C12; C12Q; C12Q1/00; C12Q1/34; C12Q1/44; G; G01; G01N; G01N2333/00
Claims Analysis
Total Claims: 16
Claim 1
Independent Claim
187 words
A reagent for measuring a lipase activity comprising a higher fatty acid ester represented by the general formula ##STR23## wherein R 1 represents an alkyl group having 9 to 17 carbon atoms; R 2 represents a hydrogen atom or a halogen atom; R 3 and R 4 , which may be the same or different, each represents a nitro group, an alkylsulfonyl group having 1 to 8 carbon atoms, an alkylsulfonyl group having 1 to 8 carbon atoms substituted by a sulfo group or an alkoxy group, or a trifluoromethyl group; G represents --SO 3 M or an atomic group having at least one --SO 3 M, where M represents sodium or potassium, or, when R 4 has a sulfonic acid group, may be a hydrogen atom; Q represents a hydrogen atom, a halogen atom, an alkyl group, an alkoxy-substituted alkyl group, an alkoxy group, an alkoxy-substituted alkoxy group, a sulfonamido group, a sulfamoyl group, a carbonamido group, a carbamoyl group, or an alkylsulfonyl group; the carbon atom number of these substitutents being 8 or less than 8; and n is an integer of 1 to 3.
Claim 2
Dependent Claim
187 words
A reagent for measuring a lipase activity comprising a higher fatty acid ester represented by the general formula ##STR23## wherein R 1 represents an alkyl group having 9 to 17 carbon atoms; R 2 represents a hydrogen atom or a halogen atom; R 3 and R 4 , which may be the same or different, each represents a nitro group, an alkylsulfonyl group having 1 to 8 carbon atoms, an alkylsulfonyl group having 1 to 8 carbon atoms substituted by a sulfo group or an alkoxy group, or a trifluoromethyl group; G represents --SO 3 M or an atomic group having at least one --SO 3 M, where M represents sodium or potassium, or, when R 4 has a sulfonic acid group, may be a hydrogen atom; Q represents a hydrogen atom, a halogen atom, an alkyl group, an alkoxy-substituted alkyl group, an alkoxy group, an alkoxy-substituted alkoxy group, a sulfonamido group, a sulfamoyl group, a carbonamido group, a carbamoyl group, or an alkylsulfonyl group; the carbon atom number of these substitutents being 8 or less than 8; and n is an integer of 1 to 3.
Claim 3
Dependent Claim
181 words
A reagent as claimed in claim 1, wherein R 2 is selected from the group consisting of hydrogen, chlorine, bromine and fluorine; R 3 and R 4 , which may be the same or different, each is selected from the group consisting of a nitro group, an alkylsulfonyl group having 1 to 8 carbon atoms, and an alkyl-sulfonyl group having 1 to 8 carbon atoms substituted by a sulfo group or an alkoxy group, G is selected from the group consisting of hydrogen, --SO 3 M, ##STR24## where the benzene ring or the naphthalene ring may have a substituent selected from the group consisting of an alkyl group, an alkoxy group, an alkoxy group substituted by an alkoxy group, a sulfonamido group, a sulfamoyl group, a carbonamido group, a carbamoyl group, and a sulfonic acid group; M represents sodium or potassium; and n is an integer of 1 to 3; Q is selected from the group consisting of hydrogen, chlorine, bromine, fluorine, and a sulfonamido group; and when G is hydrogen, R 4 is a sulfonic acid group substituted alkylsulfonyl group.
Claim 4
Dependent Claim
28 words
A reagent as claimed in claim 2, wherein R 3 and R 4 are independently selected from the group consisting of a nitro group, and a methylsulfonyl group.
Claim 5
Dependent Claim
25 words
A reagent as claimed in claim 2, wherein R 4 is a methylsulfonyl group, R 3 is a nitro group and R 2 is hydrogen.
Claim 6
Dependent Claim
22 words
The reagent as claimed in claim 1 wherein R 1 represents a straight chain alkyl group having 9 to 17 carbon atoms.
Claim 7
Dependent Claim
22 words
The reagent as claimed in claim 2 wherein R 1 represents a straight chain alkyl group having 9 to 17 carbon atoms.
Claim 8
Dependent Claim
32 words
A reagent as claimed in claim 1 comprising a higher fatty acid ester represented by the formula ##STR25## wherein Z is Na or K, and R is n--C 11 H 23 .
Claim 9
Dependent Claim
36 words
A reagent as claimed in claim 1 comprising a higher fatty acid ester represented by the formula ##STR26## wherein R is n--C 11 H 23 , n--C 13 H 27 or n--C 15 H 31 .
Claim 10
Dependent Claim
26 words
A reagent as claimed in claim 1 comprising a higher fatty acid ester represented by the formula ##STR27## wherein R is n--C 11 H 23 .
Claim 11
Dependent Claim
26 words
A reagent as claimed in claim 1 comprising a higher fatty acid ester represented by the formula ##STR28## wherein R is n--C 11 H 23 .
Claim 12
Dependent Claim
26 words
A reagent as claimed in claim 1 comprising a higher fatty acid ester represented by the formula ##STR29## wherein R is n--C 11 H 23 .
Claim 13
Dependent Claim
31 words
A reagent as claimed in claim 1 comprising a higher fatty acid ester represented by the formula ##STR30## wherein R is n--C 11 H 23 or n--C 13 H 27 .
Claim 14
Dependent Claim
26 words
A reagent as claimed in claim 1 comprising a higher fatty acid ester represented by the formula ##STR31## wherein R is n--C 11 H 23 .
Claim 15
Dependent Claim
26 words
A reagent as claimed in claim 1 comprising a higher fatty acid ester represented by the formula ##STR32## wherein R is n--C 11 H 23 .
Claim 16
Dependent Claim
26 words
A reagent as claimed in claim 1 comprising a higher fatty acid ester represented by the formula ##STR33## wherein R is n--C 11 H 23 .
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1986-10-31 | 1987-05-02 | 1987-11-01 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1990-10-31 | 1991-05-02 | 1991-11-01 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1994-10-31 | 1995-05-02 | 1995-11-01 | $7700.00 | Window Closed / Lapsed |