US4409247A
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Process for hydrolyzing lactose with immobilized lactose
Assignee: Corning Glass Works
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Filing: 1981-06-03
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Granted: 1983-10-11
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Expires: 2001-06-03
Abstract
Abstract Lactose in whey is hydrolyzed with an immobilized lactase by a process which involves heating the whey to a temperature of from about 45° to about 90° C. for at least about 15 seconds, centrifuging the heated whey while it is still warm, contacting the centrifuged whey with an immobilized lactase, cleaning the immobilized lactase, and disinfecting the immobilized lactase. Preferably, lactase is immobilized on an inorganic support, and cleaning and disinfecting are carried out respectively by contacting the immobilized lactase with a solution of protease and with a solution of substituted diethylenetriamine.
Inventors
- 👤 Jean-Luc A. G. Baret
- 👤 Luc A. Dohan
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-30 05:15
CPC Classifications
A; A23; A23C; A23C21/00; A23C21/02; A23C21/023; C; C12; C12N; C12N11/00; C12N11/14
Claims Analysis
Total Claims: 17
Claim 1
Independent Claim
164 words
A process for treating whey whereby at least a portion of the lactose contained therein is hydrolyzed into glucose and galactose by means of an immobilized lactase, which process comprises the steps of: A. heating the whey to a temperature of from about 45° to about 90° C. for at least about 15 seconds; B. centrifuging the heated whey while it is still warm; C. contacting the centrifuged whey with an immobilized lactase under conditions sufficient to hydrolyze at least a portion of the lactose contained therein into glucose and galatose; D. cleaning the immobilized lactase by contacting the immobilized lactase with a solution of a protease which removes protein coating the immobilized lactase without significant deleterious effects on the enzymatic activity of the immobilized lactase; and E. disinfecting the immobilized lactase by contacting the immobilized lactase with a disinfecting solution which substantially kills the contaminating microorganisms without significant deleterious effects on the immobilized lactase; wherein the lactase is immobilized on an inorganic support.
Claim 2
Dependent Claim
164 words
A process for treating whey whereby at least a portion of the lactose contained therein is hydrolyzed into glucose and galactose by means of an immobilized lactase, which process comprises the steps of: A. heating the whey to a temperature of from about 45° to about 90° C. for at least about 15 seconds; B. centrifuging the heated whey while it is still warm; C. contacting the centrifuged whey with an immobilized lactase under conditions sufficient to hydrolyze at least a portion of the lactose contained therein into glucose and galatose; D. cleaning the immobilized lactase by contacting the immobilized lactase with a solution of a protease which removes protein coating the immobilized lactase without significant deleterious effects on the enzymatic activity of the immobilized lactase; and E. disinfecting the immobilized lactase by contacting the immobilized lactase with a disinfecting solution which substantially kills the contaminating microorganisms without significant deleterious effects on the immobilized lactase; wherein the lactase is immobilized on an inorganic support.
Claim 3
Dependent Claim
23 words
The process of claim 1 in which the time of heating in step A is from about 15 minutes to about one hour.
Claim 4
Dependent Claim
31 words
The process of claim 1 or 2 in which the solution of a protease has an enzymatic activity of from about 0.1 to about 10 Anson units per liter of solution.
Claim 5
Dependent Claim
45 words
The process of claim 1 or 2 in which step E is accomplished by contacting the immobilized lactase with a dilute aqueous solution of at least one substituted diethylenetriamine which is selected from the group consisting of dioctyldiethylenetriamine and a mixture of dioctyldiethylenetriamine and trioctyldiethylenetriamine.
Claim 6
Dependent Claim
28 words
The process of claims 1 or 2 in which step A is carried out with a whey previously demineralized to an extent of up to about 90 percent.
Claim 7
Dependent Claim
27 words
The process of claims 1 or 2 in which prior to step C the whey is acidified to a pH of from about 3.5 to about 6.
Claim 8
Dependent Claim
17 words
The process of claims 1 or 2 in which prior to step A the whey is clarified.
Claim 9
Dependent Claim
159 words
A process for treating acid whey whereby at least a portion of the lactose contained therein is hydrolyzed into glucose and galactose by means of an immobilized lactase, which process comprises the steps of: A. clarifying the whey; B. heating the whey to a temperature of from about 45° to about 90° C. for at least about 15 seconds; C. contacting the heated whey with an immobilized lactase under conditions sufficient to hydrolyze at least a portion of the lactose contained therein into glucose and galactose; D. cleaning the immobilized lactase by contacting the immobilized lactase with a solution of a protease which removes protein coating the immobilized lactase without significant deleterious effects on the enzymatic activity of the immobilized lactase; and E. disinfecting the immobilized lactase by contacting the immobilized lactase with a disinfecting solution which substantially kills the contaminating microorganisms without significant deleterious effects on the immobilized lactase; wherein the lactase is immobilized on an inorganic support.
Claim 10
Dependent Claim
159 words
A process for treating acid whey whereby at least a portion of the lactose contained therein is hydrolyzed into glucose and galactose by means of an immobilized lactase, which process comprises the steps of: A. clarifying the whey; B. heating the whey to a temperature of from about 45° to about 90° C. for at least about 15 seconds; C. contacting the heated whey with an immobilized lactase under conditions sufficient to hydrolyze at least a portion of the lactose contained therein into glucose and galactose; D. cleaning the immobilized lactase by contacting the immobilized lactase with a solution of a protease which removes protein coating the immobilized lactase without significant deleterious effects on the enzymatic activity of the immobilized lactase; and E. disinfecting the immobilized lactase by contacting the immobilized lactase with a disinfecting solution which substantially kills the contaminating microorganisms without significant deleterious effects on the immobilized lactase; wherein the lactase is immobilized on an inorganic support.
Claim 11
Dependent Claim
23 words
The process of claim 8 in which the time of heating in step B is from about 15 minutes to about one hour.
Claim 12
Dependent Claim
31 words
The process of claim 8 or 9 in which the solution of a protease has an enzymatic activity of from about 0.1 to about 10 Anson units per liter of solution.
Claim 13
Dependent Claim
45 words
The process of claim 8 or 9 in which step E is accomplished by contacting the immobilized lactase with a dilute aqueous solution of at least one substituted diethylenetriamine which is selected from the group consisting of dioctyldiethylenetriamine and a mixture of dioctyldiethylenetriamine and trioctyldiethylenetriamine.
Claim 14
Dependent Claim
28 words
The process of claims 8 or 9 in which step B is carried out with a whey previously demineralized to an extent of up to about 90 percent.
Claim 15
Dependent Claim
55 words
A process for cleaning an immobilized lactase having whey protein deposited thereon, which process comprises contacting the immobilized lactase with a solution of a protease which removes the whey protein coating the immobilized lactase without significant deleterious effects on the enzymatic activity of the immobilized lactase, wherein the lactase is immobilized on an inorganic support.
Claim 16
Dependent Claim
55 words
A process for cleaning an immobilized lactase having whey protein deposited thereon, which process comprises contacting the immobilized lactase with a solution of a protease which removes the whey protein coating the immobilized lactase without significant deleterious effects on the enzymatic activity of the immobilized lactase, wherein the lactase is immobilized on an inorganic support.
Claim 17
Dependent Claim
29 words
The method of claim 13 in which the solution of a protease has an enzymatic activity of from about 0.1 to about 10 Anson units per liter of solution.
References Cited (Backward Citations)
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1986-10-10 | 1987-04-11 | 1987-10-11 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1990-10-10 | 1991-04-11 | 1991-10-11 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1994-10-10 | 1995-04-11 | 1995-10-11 | $7700.00 | Window Closed / Lapsed |