US4407965A
Expired / Lapsed
US
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Process for preparing antibody
Assignee: Otsuka Pharmaceutical Co Ltd
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Filing: 1981-05-21
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Granted: 1983-10-04
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Expires: 2001-05-21
Abstract
Abstract A process for preparing a gut glucagon antigen composed of a peptide-carrier complex is disclosed, which comprises using as hapten a peptide represented by the following general formula; R-Lys-Arg-Asn-Lys-Asn-Asn-Ile-Ala-OH wherein R represents a hydrogen atom, an amino acid group or a peptide group containing 2 to 20 amino acid groups, and reacting it with a carrier in the presence of a binding agent for binding the hapten and the carrier to each other. Also, a process for preparing a gut glucagon antibody, which comprises administering the antigen described to mammals, and collecting the thus-produced antibody, is disclosed.
Inventors
- 👤 Noboru Yanaihara
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-24 16:18
CPC Classifications
C; C07; C07K; C07K14/00; C07K14/435; C07K14/575; C07K14/605; C07K16/00; C07K16/18; C07K16/26; Y; Y10; Y10S; Y10S436/00; Y10S436/822; Y10S436/823; Y10S530/00; Y10S530/806; Y10S930/00; Y10S930/01; Y10S930/26
Claims Analysis
Total Claims: 9
Claim 1
Independent Claim
119 words
A gut glucagon-specific antibody prepared by administering to a mammal an antigen composed of a peptide-carrier complex obtained by (a) reacting a peptide as a hapten with a carrier in the presence of a binding agent for binding the hapten and the carrier to each other, and (b) collecting the resulting antibody, wherein the peptide is represented by the general formula: R-Lys-Arg-Asn-Lys-Asn-Asn-Ile-Ala-OH wherein R represents a member selected from the group consisting of H, H-Arg-, H-Trp-, H-Asn-, H-Ala, H-Val-, H-Met, H-Leu-, H-Phe-, H-Lys-, H-Thr-, H-Ile-, ##STR3## H-Gly-, H-His-, H-Pro-, H-Cys, H-Gly-Lys-, H-Asn-Tyr-, H-Met-Thr-, H-Met-Asn-, H-Leu-Thr-, H-Trp-Thr-, H-Phe-Val-, H-Gln-Ala-, H-Asp-Arg-, H-Leu-Tyr-, H-Ser-Lys-, H-Met-Asn-Thr-, H-Leu-Met-Asn-Thr-, H-Trp-Leu-Met-Asn-Thr-, H-Gln-Trp-Leu-Met-Asn-Thr-, H-Val-Gln-Trp-Leu-Met-Asn-Thr-, H-Phe-Val-Gln-Trp-Leu-Met-Asn-Thr-, H-Asp-Phe-Val-Gln-Trp-Leu-Met-Asn-Thr-, H-Gln-Asp-Phe-Val-Gln-Trp-Leu-Met-Asn-Thr-, H-Ala-Gln-Asp-Phe-Val-Gln-Trp-Leu-Met-Asn-Thr-, H-Arg-Ala-Gln-Asp-Phe-Val-Gln-Trp-Leu-Met-Asn-Thr-, H-Arg-Arg-Ala-Gln-Asp-Phe-Val-Gln-Trp-Leu-Met-Asn-Thr-, H-Ser-Arg-Arg-Ala-Gln-Asp-Phe-Val-Gln-Trp-Leu-Met-Asn-Thr-, H-Asp-Ser-Arg-Arg-Ala-Gln-Asp-Phe-Val-Gln-Trp-Leu-Met-Asn-Thr-, H-Leu-Asp-Ser-Arg-Arg-Ala-Gln-Asp-Phe-Val-Gln-Trp-Leu-Met-Asn-Thr-, H-Tyr-Leu-Asp-Ser-Arg-Arg-Ala-Gln-Asp-Phe-Val-Gln-Trp-Leu-Met-Asn-Thr-, H-Lys-Tyr-Leu-Asp-Ser-Arg-Arg-Ala-Gln-Asp-Phe-Val-Gln-Trp-Leu-Met-Asn-Thr-, and H-Ser-Lys-Tyr-Leu-Asp-Ser-Arg-Arg-Ala-Gln-Asp-Phe-Val-Gln-Trp-Leu-Met-Asn-Thr-.
Claim 2
Dependent Claim
119 words
A gut glucagon-specific antibody prepared by administering to a mammal an antigen composed of a peptide-carrier complex obtained by (a) reacting a peptide as a hapten with a carrier in the presence of a binding agent for binding the hapten and the carrier to each other, and (b) collecting the resulting antibody, wherein the peptide is represented by the general formula: R-Lys-Arg-Asn-Lys-Asn-Asn-Ile-Ala-OH wherein R represents a member selected from the group consisting of H, H-Arg-, H-Trp-, H-Asn-, H-Ala, H-Val-, H-Met, H-Leu-, H-Phe-, H-Lys-, H-Thr-, H-Ile-, ##STR3## H-Gly-, H-His-, H-Pro-, H-Cys, H-Gly-Lys-, H-Asn-Tyr-, H-Met-Thr-, H-Met-Asn-, H-Leu-Thr-, H-Trp-Thr-, H-Phe-Val-, H-Gln-Ala-, H-Asp-Arg-, H-Leu-Tyr-, H-Ser-Lys-, H-Met-Asn-Thr-, H-Leu-Met-Asn-Thr-, H-Trp-Leu-Met-Asn-Thr-, H-Gln-Trp-Leu-Met-Asn-Thr-, H-Val-Gln-Trp-Leu-Met-Asn-Thr-, H-Phe-Val-Gln-Trp-Leu-Met-Asn-Thr-, H-Asp-Phe-Val-Gln-Trp-Leu-Met-Asn-Thr-, H-Gln-Asp-Phe-Val-Gln-Trp-Leu-Met-Asn-Thr-, H-Ala-Gln-Asp-Phe-Val-Gln-Trp-Leu-Met-Asn-Thr-, H-Arg-Ala-Gln-Asp-Phe-Val-Gln-Trp-Leu-Met-Asn-Thr-, H-Arg-Arg-Ala-Gln-Asp-Phe-Val-Gln-Trp-Leu-Met-Asn-Thr-, H-Ser-Arg-Arg-Ala-Gln-Asp-Phe-Val-Gln-Trp-Leu-Met-Asn-Thr-, H-Asp-Ser-Arg-Arg-Ala-Gln-Asp-Phe-Val-Gln-Trp-Leu-Met-Asn-Thr-, H-Leu-Asp-Ser-Arg-Arg-Ala-Gln-Asp-Phe-Val-Gln-Trp-Leu-Met-Asn-Thr-, H-Tyr-Leu-Asp-Ser-Arg-Arg-Ala-Gln-Asp-Phe-Val-Gln-Trp-Leu-Met-Asn-Thr-, H-Lys-Tyr-Leu-Asp-Ser-Arg-Arg-Ala-Gln-Asp-Phe-Val-Gln-Trp-Leu-Met-Asn-Thr-, and H-Ser-Lys-Tyr-Leu-Asp-Ser-Arg-Arg-Ala-Gln-Asp-Phe-Val-Gln-Trp-Leu-Met-Asn-Thr-.
Claim 3
Dependent Claim
46 words
The antibody as claimed in claim 1, wherein said carrier is a member selected from the group consisting of animal serum albumins, animal serum globulins, animal thyroglobulins, animal hemoglobins, animal hemocyanins, protein extract from ascaris, polylysine, polyglutamic acid, lysine-glutamic acid copolymer, lysine-containing copolymer, and ornithine-containing copolymer.
Claim 4
Dependent Claim
40 words
The antibody as claimed in claim 1, wherein said binding agent is a member selected from the group consisting of glyoxal, malondialdehyde, glutaraldehyde, succinaldehyde, adipoaldehyde, N-N'-o-phenylenedimaleimide, N,N'-m-phenylenediamaleimide, m-maleimidobenzoyl-N-hydroxysuccinimide ester, 4-(maleimidemethyl)-cyclohexane-1-carboxyl-N'-hydroxy-succinimide ester, N,N'-dicyclohexyl carbodiimide, N-ethyl-N'-dimethylaminocarbodiimide, 1-ethyl-3-diisopropylamino carbodiimide, 1-cyclohexyl-3-(2-morpholinyl-4-ethyl)-carbodiimide, and p-diazonium-phenylacetic acid.
Claim 5
Dependent Claim
29 words
The antibody as claimed in claim 1, wherein R represents a member selected from the group consisting of H, H-Gly-, H-Ala-, H-Tyr-, H-Lys-, H-Gly-Lys-, H-Leu-Met-Asn-Thr-, H-Val-Gln-Trp-Leu-Met-Asn-Thr-, H-Arg-Arg-Ala-Gln-Asp-Phe-Val-Gln-Trp-Leu-Met-Asn-Thr-, and H-Ser-Lys-Tyr-Leu-Asp-Ser-Arg-Arg-Ala-Gln-Asp-Phe-Val-Gln-Trp-Leu-Met-Asn-Thr-.
Claim 6
Dependent Claim
23 words
The antibody as claimed in claim 4, wherein R represents H-Arg-Arg-Ala-Gln-Asp-Phe-Val-Gln-Trp-Leu-Met-Asn-Thr-, said carrier is bovine serum albumin and said binding agent is glutaraldehyde.
Claim 7
Dependent Claim
23 words
The antibody as claimed in claim 4, wherein R represents H-Ser-Lys-Thr-Leu-Asp-Ser-Arg-Arg-Ala-Gln-Asp-Phe-Val-Gln-Trp-Leu-Met-Asn-Thr-, said carrier is bovine serum albumin and said binding agent is glutaraldehyde.
Claim 8
Dependent Claim
23 words
The antibody as claimed in claim 4, wherein R represents H-Leu-Met-Asn-Thr-, said carrier is bovine serum albumin and said binding agent is glutaraldehyde.
Claim 9
Dependent Claim
25 words
The antibody as claimed in claim 4, wherein R represents a hydrogen atom, said carrier is bovine serum albumin and said binding agent is glutaraldehyde.
References Cited (Backward Citations)
US4221777A
Cited
US4206199A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1986-10-03 | 1987-04-04 | 1987-10-04 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1990-10-03 | 1991-04-04 | 1991-10-04 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1994-10-03 | 1995-04-04 | 1995-10-04 | $7700.00 | Window Closed / Lapsed |