US4396630A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Process for preparing lipoid-biologically active particles
Assignee: Human Oltoanyagtermelo Es Kutato Intezet
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Filing: 1980-12-19
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Granted: 1983-08-02
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Expires: 2000-12-19
Abstract
Abstract Adjuvant particles are prepared from lipoid-soluble substances, and compositions comprising the particles and biologically active substances adsorbed thereon are provided. The adjuvant systems are capable of binding a wide variety of biologically active substances, and can be used in vivo and in vitro as well.
Inventors
- 👤 Zoltan Riedl
- 👤 Laszlo Sztankov
- 👤 Istvan Horvath
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-30 17:59
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/58; G01N33/585; G01N33/586; A; A61; A61K; A61K9/00; A61K9/10; A61K9/127; A61K9/1271; A61K9/1277; C; C07; C07K; C07K16/00; C07K16/18; C07K16/26; G01N33/53; G01N33/531; A61K38/00
Claims Analysis
Total Claims: 6
Claim 1
Independent Claim
202 words
Process for the preparation of particles from lipoid-soluble substances and compositions containing said particles and biologically active substances bound to them, which comprises admixing an egg lecithin and cholesterol dissolved in absolute alcohol, with (a) a biologically active substance taken up in an aqueous electrolyte having a pH of 2 to 10, in a volume ratio of 1:1000-9:1, stirring the reaction mixture for at least one minute, allowing it to stand for at most 7 days and separating the particles precipitated from the solution; or (b) an aqueous electrolyte having a pH of 2 to 10, in a volume ratio of 1:1000 to 9:1, and isolating the precipitated particles (c) an aqueous electrolyte solution in a volume ratio of 1:1000 to 9:1, isolating the precipitated particles, adding them to a biologically active substance taken up in an aqueous electrolyte solution having a pH of 2 to 10, stirring the solution obtained for at least two minutes, allowing to stand for at most 7 days and isolating the particles formed, or (d) adding to the particles prepared according to variant (b) a biologically active substance taken up in an aqueous electrolyte solution having a pH of 2 to 10 and forming a suspension.
Claim 2
Dependent Claim
202 words
Process for the preparation of particles from lipoid-soluble substances and compositions containing said particles and biologically active substances bound to them, which comprises admixing an egg lecithin and cholesterol dissolved in absolute alcohol, with (a) a biologically active substance taken up in an aqueous electrolyte having a pH of 2 to 10, in a volume ratio of 1:1000-9:1, stirring the reaction mixture for at least one minute, allowing it to stand for at most 7 days and separating the particles precipitated from the solution; or (b) an aqueous electrolyte having a pH of 2 to 10, in a volume ratio of 1:1000 to 9:1, and isolating the precipitated particles (c) an aqueous electrolyte solution in a volume ratio of 1:1000 to 9:1, isolating the precipitated particles, adding them to a biologically active substance taken up in an aqueous electrolyte solution having a pH of 2 to 10, stirring the solution obtained for at least two minutes, allowing to stand for at most 7 days and isolating the particles formed, or (d) adding to the particles prepared according to variant (b) a biologically active substance taken up in an aqueous electrolyte solution having a pH of 2 to 10 and forming a suspension.
Claim 3
Dependent Claim
14 words
A process as claimed in claim 1, which comprises separating the particles by centrifugation.
Claim 4
Dependent Claim
20 words
A process as claimed in claim 1, which comprises lyophilizing and sterilizing by irradiation the product prepared by variant (a).
Claim 5
Dependent Claim
20 words
A process as claimed in claim 1, which comprises lyophilizing and sterilizing by irradiation the product prepared by variant (c).
Claim 6
Dependent Claim
20 words
A process as claimed in claim 1, which comprises lyophilizing and sterilizing by irradiation the product prepared by variant (b).
References Cited (Backward Citations)
US3265629A
Cited
JPS5126213A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1986-08-01 | 1987-01-31 | 1987-08-02 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1990-08-01 | 1991-01-31 | 1991-08-02 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1994-08-01 | 1995-01-31 | 1995-08-02 | $7700.00 | Window Closed / Lapsed |