← Back to Patent Explorer
US4396630A Expired / Lapsed US ⏱️ 0.0 years remaining

Process for preparing lipoid-biologically active particles

Assignee: Human Oltoanyagtermelo Es Kutato Intezet
•
Filing: 1980-12-19
•
Granted: 1983-08-02
•
Expires: 2000-12-19
📄 Original PDF 🌐 Google Patents 🔄 Sync Now

Abstract

Abstract Adjuvant particles are prepared from lipoid-soluble substances, and compositions comprising the particles and biologically active substances adsorbed thereon are provided. The adjuvant systems are capable of binding a wide variety of biologically active substances, and can be used in vivo and in vitro as well.

Inventors

  • 👤 Zoltan Riedl
  • 👤 Laszlo Sztankov
  • 👤 Istvan Horvath

Bibliographic Details

Application Number —
Jurisdiction US
Data Source google_patents
Last Synchronized 2026-10-30 17:59
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/58; G01N33/585; G01N33/586; A; A61; A61K; A61K9/00; A61K9/10; A61K9/127; A61K9/1271; A61K9/1277; C; C07; C07K; C07K16/00; C07K16/18; C07K16/26; G01N33/53; G01N33/531; A61K38/00

Claims Analysis

Total Claims: 6
Claim 1
Independent Claim 202 words
Process for the preparation of particles from lipoid-soluble substances and compositions containing said particles and biologically active substances bound to them, which comprises admixing an egg lecithin and cholesterol dissolved in absolute alcohol, with (a) a biologically active substance taken up in an aqueous electrolyte having a pH of 2 to 10, in a volume ratio of 1:1000-9:1, stirring the reaction mixture for at least one minute, allowing it to stand for at most 7 days and separating the particles precipitated from the solution; or (b) an aqueous electrolyte having a pH of 2 to 10, in a volume ratio of 1:1000 to 9:1, and isolating the precipitated particles (c) an aqueous electrolyte solution in a volume ratio of 1:1000 to 9:1, isolating the precipitated particles, adding them to a biologically active substance taken up in an aqueous electrolyte solution having a pH of 2 to 10, stirring the solution obtained for at least two minutes, allowing to stand for at most 7 days and isolating the particles formed, or (d) adding to the particles prepared according to variant (b) a biologically active substance taken up in an aqueous electrolyte solution having a pH of 2 to 10 and forming a suspension.
Claim 2
Dependent Claim 202 words
Process for the preparation of particles from lipoid-soluble substances and compositions containing said particles and biologically active substances bound to them, which comprises admixing an egg lecithin and cholesterol dissolved in absolute alcohol, with (a) a biologically active substance taken up in an aqueous electrolyte having a pH of 2 to 10, in a volume ratio of 1:1000-9:1, stirring the reaction mixture for at least one minute, allowing it to stand for at most 7 days and separating the particles precipitated from the solution; or (b) an aqueous electrolyte having a pH of 2 to 10, in a volume ratio of 1:1000 to 9:1, and isolating the precipitated particles (c) an aqueous electrolyte solution in a volume ratio of 1:1000 to 9:1, isolating the precipitated particles, adding them to a biologically active substance taken up in an aqueous electrolyte solution having a pH of 2 to 10, stirring the solution obtained for at least two minutes, allowing to stand for at most 7 days and isolating the particles formed, or (d) adding to the particles prepared according to variant (b) a biologically active substance taken up in an aqueous electrolyte solution having a pH of 2 to 10 and forming a suspension.
Claim 3
Dependent Claim 14 words
A process as claimed in claim 1, which comprises separating the particles by centrifugation.
Claim 4
Dependent Claim 20 words
A process as claimed in claim 1, which comprises lyophilizing and sterilizing by irradiation the product prepared by variant (a).
Claim 5
Dependent Claim 20 words
A process as claimed in claim 1, which comprises lyophilizing and sterilizing by irradiation the product prepared by variant (c).
Claim 6
Dependent Claim 20 words
A process as claimed in claim 1, which comprises lyophilizing and sterilizing by irradiation the product prepared by variant (b).

References Cited (Backward Citations)

US3265629A Cited
JPS5126213A Cited

USPTO Annuity & Maintenance Windows

Annuity Stage Window Opens Payment Deadline Surcharge Period Until Estimated Fee Status
3.5 Years (Year 4) 1986-08-01 1987-01-31 1987-08-02 $2000.00 Window Closed / Lapsed
7.5 Years (Year 8) 1990-08-01 1991-01-31 1991-08-02 $3760.00 Window Closed / Lapsed
11.5 Years (Year 12) 1994-08-01 1995-01-31 1995-08-02 $7700.00 Window Closed / Lapsed

Assigned Categories & Tags

No categories assigned to this patent yet.

Assign Category Tag