US4395397A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Apparatus and method for killing unwanted cells
Assignee: Dana Farber Cancer Institute Inc
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Filing: 1981-09-17
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Granted: 1983-07-26
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Expires: 2001-09-17
Abstract
Abstract Method and apparatus for removing, from a flowing liquid stream containing a suspension of living cells, a subpopulation of unwanted cells having one or more characteristics distinguishing them from the rest of the living cells. The method includes detecting the presence of the unwanted cells, generating signals in response to the presence of the unwanted cells, and providing pulses of laser light, in response to the generated signals, to impinge on and kill the unwanted cells.
Inventors
- 👤 Howard M. Shapiro
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-26 02:52
CPC Classifications
C; C12; C12M; C12M35/00; C12M35/02; A; A61; A61K; A61K41/00; A61K41/10; A61K41/17; C12M47/00; C12M47/06
Claims Analysis
Total Claims: 6
Claim 1
Independent Claim
107 words
Apparatus for killing, in a flowing liquid stream containing a suspension of living cells, a subpopulation of unwanted cells having one or more characteristics distinguishing them from the rest of said living cells, said apparatus comprising means for detecting the presence of unwanted cells in a flowing stream containing a suspension of living cells, means for generating signals in response to the presence of said unwanted cells in said flowing stream, and means downstream from said detecting means and responsive to said generated signals, for providing pulses of laser light focused on said flowing stream to impinge on and kill said unwanted cells in said flowing stream.
Claim 2
Dependent Claim
107 words
Apparatus for killing, in a flowing liquid stream containing a suspension of living cells, a subpopulation of unwanted cells having one or more characteristics distinguishing them from the rest of said living cells, said apparatus comprising means for detecting the presence of unwanted cells in a flowing stream containing a suspension of living cells, means for generating signals in response to the presence of said unwanted cells in said flowing stream, and means downstream from said detecting means and responsive to said generated signals, for providing pulses of laser light focused on said flowing stream to impinge on and kill said unwanted cells in said flowing stream.
Claim 3
Independent Claim
96 words
A method for killing, in a flowing liquid stream containing a suspension of living cells, a subpopulation of unwanted cells having one or more characteristics distinguishing them from the rest of said living cells, said method comprising detecting the presence of unwanted cells in a flowing stream containing a suspension of living cells, generating signals in response to the presence of said unwanted cells in said flowing stream, and in response to said signals, providing pulses of laser light focused on said flowing stream to impinge on and kill said unwanted cells in said flowing stream.
Claim 4
Independent Claim
96 words
A method for killing, in a flowing liquid stream containing a suspension of living cells, a subpopulation of unwanted cells having one or more characteristics distinguishing them from the rest of said living cells, said method comprising detecting the presence of unwanted cells in a flowing stream containing a suspension of living cells, generating signals in response to the presence of said unwanted cells in said flowing stream, and in response to said signals, providing pulses of laser light focused on said flowing stream to impinge on and kill said unwanted cells in said flowing stream.
Claim 5
Dependent Claim
22 words
The method of claim 2, wherein: said unwanted cells are cancer cells, and said cancer cells are labelled with attached fluorescent antibody.
Claim 6
Dependent Claim
24 words
The method of claim 3, wherein: said cancer cells are leukemia cells, and said antibody is a monoclonal antibody specific for said leukemia cells.
References Cited (Backward Citations)
US3817703A
Cited
US3941670A
Cited
US3826364A
Cited
US3826364B1
Cited
DE2300677A1
Cited
US4042325A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1986-07-25 | 1987-01-24 | 1987-07-26 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1990-07-25 | 1991-01-24 | 1991-07-26 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1994-07-25 | 1995-01-24 | 1995-07-26 | $7700.00 | Window Closed / Lapsed |