US4391749A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Method for the purification of collagens
Assignee: Sanford Burnham Prebys Medical Discovery Institute
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Filing: 1981-10-19
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Granted: 1983-07-05
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Expires: 2001-10-19
Abstract
Abstract Collagen affinity matrices are prepared, useful for separating collagenous substances from other high molecular weight proteinaceous substances. Fibronectin is fragmentated chemically or by enzymatic digestion, etc. Fragments of fibronectin, which retain the collagen-binding site but are severed from other binding sites of intact fibronectin, are selected and coupled to solid support material.
Inventors
- 👤 Eva S. Engvall
- 👤 Erkki I. Ruoslahti
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-02 16:21
CPC Classifications
B; B01; B01D; B01D15/00; B01D15/08; B01D15/26; B01D15/38; B01D15/3804; B01J; B01J20/00; B01J20/281; B01J20/286; B01J20/30; B01J20/32; B01J20/3202; B01J20/3204; B01J20/3206; B01J20/3208; B01J20/321; B01J20/3212; B01J20/3231; B01J20/3242; B01J20/3268; B01J20/3272; B01J20/3274; C; C07; C07K; C07K14/00; C07K14/435; C07K14/78; B01J2220/00; B01J2220/50; B01J2220/54
Claims Analysis
Total Claims: 12
Claim 1
Independent Claim
76 words
Affinity matrix material for separating collagen from other high molecular weight proteinaceous substances comprising: solid chromatographic support material; and at least one fragment of fibronectin which retains thereupon collagen binding sites, said fragment having been severed from other binding sites of intact fibronectin, said fragment being coupled with said solid chromatographic support material leaving collagen binding sites thereof exposed to selectively bind collagenous material when used chromatographically without binding interfering high molecular weight non-collagenous proteinaceous substances.
Claim 2
Dependent Claim
76 words
Affinity matrix material for separating collagen from other high molecular weight proteinaceous substances comprising: solid chromatographic support material; and at least one fragment of fibronectin which retains thereupon collagen binding sites, said fragment having been severed from other binding sites of intact fibronectin, said fragment being coupled with said solid chromatographic support material leaving collagen binding sites thereof exposed to selectively bind collagenous material when used chromatographically without binding interfering high molecular weight non-collagenous proteinaceous substances.
Claim 3
Dependent Claim
40 words
The affinity matrix material of claim 1 wherein at least one of said fragments has a molecular weight of about 30 kd characterized as having the collagen specificity of such molecular weight fragment resulting from digestion of fibronectin with trypsin.
Claim 4
Dependent Claim
39 words
The affinity matrix material of claim 1 wherein at least one of said fragments has a molecular weight of about 45 kd characterized as having collagen specificity of such molecular weight fragment resulting from digestion of fibronectin with chymotrypsin.
Claim 5
Dependent Claim
40 words
The affinity matrix material of claim 1 wherein at least one of said fragments consists essentially of the sites of fibronectin which bind collagen, said fragment not including the sites of fibronectin which bind other high molecular weight proteinaceous substances.
Claim 6
Dependent Claim
19 words
The affinity matrix material of claim 1, 2, 3 or 4 wherein the solid chromatographic support material is Sepharose.
Claim 7
Dependent Claim
24 words
The affinity matrix material of claim 1, 2, 3 or 4 wherein the solid chromatographic support material is agarose, dextran, cellulose, polystyrene or glass.
Claim 8
Dependent Claim
113 words
In the chromatographic method for separating proteinaceous substances from one another, in which a mixture of such substances is passed by way of a solution over a chromatographic material, the improvement comprising separating collagen from other proteinaceous sustances by passing the proteinaceous material containing solution over an affinity matrix material comprising: solid chromatographic support material; and at least one fragment of fibronectin which retains thereupon collagen binding sites, said fragment having been severed from other binding sites of intact fibronectin, said fragment being coupled with said solid chromatographic support material leaving callogen binding sites thereof exposed to selectively bind collagenous material when used chromatographically without binding interfering high molecular weight non-collagenous proteinaceous substances.
Claim 9
Dependent Claim
113 words
In the chromatographic method for separating proteinaceous substances from one another, in which a mixture of such substances is passed by way of a solution over a chromatographic material, the improvement comprising separating collagen from other proteinaceous sustances by passing the proteinaceous material containing solution over an affinity matrix material comprising: solid chromatographic support material; and at least one fragment of fibronectin which retains thereupon collagen binding sites, said fragment having been severed from other binding sites of intact fibronectin, said fragment being coupled with said solid chromatographic support material leaving callogen binding sites thereof exposed to selectively bind collagenous material when used chromatographically without binding interfering high molecular weight non-collagenous proteinaceous substances.
Claim 10
Dependent Claim
39 words
The chromatographic method of claim 7 wherein at least one of said fragments has a molecular weight of about 30 kd characterized as having the collagen specificity of such molecular weight fragment resulting from digestion of fibronectin with trypsin.
Claim 11
Dependent Claim
38 words
The chromatographic method of claim 7 wherein at least one of said fragments has a molecular weight of about 45 kd characterized as having collagen specificity of such molecular weight fragment resulting from digestion of fibronectin with chymotrypsin.
Claim 12
Dependent Claim
39 words
The chromatographic method of claim 7 wherein at least one of said fragments consists essentially of the sites of fibronectin which bind collagen, said fragment not including the sites of fibronectin which bind other high molecular weight proteinaceous substances.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1986-07-04 | 1987-01-03 | 1987-07-05 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1990-07-04 | 1991-01-03 | 1991-07-05 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1994-07-04 | 1995-01-03 | 1995-07-05 | $7700.00 | Window Closed / Lapsed |