Cytocentrifuge

Claim 2

A cytocentrifuge comprising (a) a rotating carrier; and (b) a plurality of assemblies mounted on the carrier for rotation therewith and for removal therefrom as a unit, each assembly including (1) a holder comprising a channel member having a web and a flange, the channel member retaining a slide providing a receiving surface and a filter card overlying the slide and providing an absorbent seal thereover, (2) a sample chamber comprising a tube having an end flange shaped to be received by the holder in overlying relationship to the slide and filter card; and (3) said holder further comprising clamping means adapted to engage the end flange of the sample chamber for urging the same towards the web of the channel member whereby the assembly of sample chamber, filter card and slide is secured.

Claim 3

The cytocentrifuge of claim 1 in which each said assembly is adapted to be supported by the rotating carrier in a manner permitting tilting of the asssembly about a horizontal axis in response to gravitational and centrifugal loads whereby when the carrier is at rest the assembly adopts a position in which the axis of the sample chamber is inclined downwardly and inwardly of the carrier, whereas under the centrifugal loads arising from rotation of the carrier, the assembly tilts to a position in which the sample chamber axis is horizontal and perpendicular to the axis of rotation.

Claim 4

A cytocentrifuge comprising (a) a rotating carrier; and (b) a plurality of assemblies mounted on the carrier for rotation therewith and for removal therefrom as a unit, each assembly including (1) a holder comprising a channel member having a web and a flange, the channel member retaining a slide providing a receiving surface and a filter card overlying the slide and providing an absorbent seal thereover, (2) a sample chamber comprising a tube having an end flange shaped to be received by the holder in overlying relationship to the slide and filter card; and (3) said holder further comprising clamping means adapted to engage the end flange of the sample chamber for urging the same towards the web of the channel member whereby the assembly of sample chamber, filter card and slide is secured, the clamping means comprising (4) a rocking element pivoted to the channel member, the channel member having two of said flanges and the rocking element having lugs extending over the flanges of the channel member to engage the end flange of the sample chamber, the rocking member being spring-loaded for rocking in a direction causing the lugs to move towards the web of the channel member whereby a clamping force is produced on the end flange of the sample chamber.

Claim 5

A cytocentrifuge comprising (a) a rotating carrier; and (b) a plurality of assemblies mounted on the carrier for rotation therewith and for removal therefrom as a unit, each assembly including (1) a holder comprising a channel member having a web and a flange, the channel member retaining a slide providing a receiving surface and a filter card overlying the slide and providing an absorbent seal thereover, (2) a sample chamber comprising a tube having an end flange shaped to be received by the holder in overlying relationship to the slide and filter card; and (3) said holder further comprising clamping means adapted to engage the end flange of the sample chamber for urging the same towards the web of the channel member whereby the assembly of sample chamber, filter card and slide is secured, the clamping means comprising (4) a rocking element pivoted to the channel member, the channel member having two of said flanges and the rocking element having lugs extending over the flanges of the channel member to engage the end flange of the sample chamber, the rocking member being spring-loaded for rocking in a direction causing the lugs to move towards the web of the channel member whereby a clamping force is produced on the end flange of the sample chamber.

Claim 6

The cytocentrifuge of claim 3 in which said rocking element carries a release screw that may be manipulated first to engage the outer face of the channel member web and thereafter forcibly to rock the rocking element in the clamp-releasing direction and to hold the rocking element in a clmap-released position to facilitate assembly and disassembly of the sample chamber, filter card and slide in the holder.

Claim 7

The cytocentrifuge of claim 3 in which said holder channel has lateral stiffening ribs along its rear or outer aspect, these ribs being recessed to provide locations for a pivot rod that extends across such ribs and through a rearwardly directed tang pressed out of the web, this pivot rod extending through side cheeks in the rocking element and carrying a hairpin spring acting respectively on the rear face of the holder web and upon the rocking element to tend to rotate the latter about the pivot rod.

Claim 8

The cytocentrifuge of claim 3 in which said lugs of the rocking element are positioned to engage the end flange of the sample chamber adjacent to a diameter of the latter.

Claim 9

The cytocentrifuge of claim 3 in which said holder channel member has an end stop at one end to locate the components in the required mutual relationship with one another and with the holder, the other end of the channel member being open to permit the components to be slid out of the channel member upon release of the said clamping means.

Claim 10

The cytocentrifuge of claim 3 in which each said sample chamber comprises a tube having one end integrally formed with a rectangular end flange, and a lateral filling orifice near its other end.

Claim 11

The cytocentrifuge of claim 8 including a funnel tube communicating with said filling orifice.

Claim 12

The cytocentrifuge of claim 9 in which said funnel tube is integrally formed with the sample chamber and also with lateral flanges that extend to join the end flange of the sample chamber along the longitudinal centerline of the end flange.

Claim 13

The cytocentrifuge of claim 3 in which the rotating carrier is formed as a plate with apertures to receive the assemblies and a central aperture to fit over a drive hub from which the carrier pate may be removed at will for cleansing and sterilization as required.

Claim 14

The cytocentrifuge of claim 12 including a containment bowl and removable cover carried by said drive hub and constituting a sealable head assembly that may be removed as a unit from the machine to permit of its being handled in a containment zone.

Claim 15

The cytocentrifuge of claim 11 in which said drive hub is formed with an internal tapered drive surface for mating with a complementarily tapered driving element on a vertical drive shaft.