US4391689A — Automated electrophoresis and staining apparatus …

Claim 1

Independent Claim 318 words

An automated electrophoresis and staining apparatus comprising a cabinet having an apertured top wall; an elongated housing spaced within said cabinet having upright front and rear walls, an end wall, a bottom wall and a platform between said front and rear walls overlying said bottom wall with said front and rear walls projecting above said top wall; an electrophoresis chamber adapted to contain a buffer solution mounted upon said platform at one end of said housing; and a series of vats mounted upon said platform arranged in a row and aligned with said chamber, adapted to contain respectively a liquid stain and a series of processing solutions; said front and rear walls having transversely aligned longitudinally spaced pairs of notches in and along their upper edges; said pairs of notches being over and in alignment with the centerline of each of said vats and chamber respectively; a plate holder rack overlying said electrophoresis chamber including a horizontal frame and at its ends a pair of outwardly projecting inverted L-shaped arms respectively nested in the pair of notches in registry with said chamber; and adapted for supporting one or a plurality of upright electrophoresis plates onto which has been applied a sample for electrophoretic fractionization; said plates selected from the group consisting of cellulose acetate, cellulose esters, agarose, acrylamide gel and any other media suitable for electrophoresis; said plate or plates being nested within said chamber within an electrophoretic circuit between a cathode and anode for a predetermined period; and a power operated lift and transfer assembly within said cabinet outwardly of said front and rear walls adapted to progressively lift, transfer and lower said plate holder and plates from said chamber and progressively into each of said pairs of notches and underlying vats for a predetermined period successively in a linear stepping motion; whereby said plates in an upright position are immersed into the fluids within said vats.

Claim 2

Dependent Claim 318 words

An automated electrophoresis and staining apparatus comprising a cabinet having an apertured top wall; an elongated housing spaced within said cabinet having upright front and rear walls, an end wall, a bottom wall and a platform between said front and rear walls overlying said bottom wall with said front and rear walls projecting above said top wall; an electrophoresis chamber adapted to contain a buffer solution mounted upon said platform at one end of said housing; and a series of vats mounted upon said platform arranged in a row and aligned with said chamber, adapted to contain respectively a liquid stain and a series of processing solutions; said front and rear walls having transversely aligned longitudinally spaced pairs of notches in and along their upper edges; said pairs of notches being over and in alignment with the centerline of each of said vats and chamber respectively; a plate holder rack overlying said electrophoresis chamber including a horizontal frame and at its ends a pair of outwardly projecting inverted L-shaped arms respectively nested in the pair of notches in registry with said chamber; and adapted for supporting one or a plurality of upright electrophoresis plates onto which has been applied a sample for electrophoretic fractionization; said plates selected from the group consisting of cellulose acetate, cellulose esters, agarose, acrylamide gel and any other media suitable for electrophoresis; said plate or plates being nested within said chamber within an electrophoretic circuit between a cathode and anode for a predetermined period; and a power operated lift and transfer assembly within said cabinet outwardly of said front and rear walls adapted to progressively lift, transfer and lower said plate holder and plates from said chamber and progressively into each of said pairs of notches and underlying vats for a predetermined period successively in a linear stepping motion; whereby said plates in an upright position are immersed into the fluids within said vats.

Claim 3

Dependent Claim 91 words

In the electrophoresis apparatus of claim 1, there being an upright plate drying chamber within said housing, above said platform, between the last of said vats and the housing end wall; and power means within said housing, below said platform for supplying heated air to the base of said drying chamber for upward movement therethrough; there being an additional pair of housing wall notches in registry with said drying chamber; said transfer assembly adapted to position and support said rack within said latter notches for suspension thereof within said drying chamber.

Claim 4

Dependent Claim 33 words

In the electrophoresis apparatus of claim 1, said electrophoresis chamber including a cover pivoted upon said housing, spanning its front and rear walls, overlying said lift and transfer assembly and closing said chamber.

Claim 5

Dependent Claim 74 words

In the electrophoresis apparatus of claim 3, an elongated sponge holder underlying said cover adapted to support an elongated buffer moistened sponge; there being an elongated buffer moistened sponge within the bottom of said chamber; said electrophoretic circuit including electrodes connected to a power source and in engagement with said sponges respectively, said sample mounted plates at their tops and bottoms being in engagement with said sponges for completing the circuit between said electrodes.

Claim 6

Dependent Claim 36 words

In the electrophoresis apparatus of claim 4, a pair of normally opened tilt switches mounted upon said cover and connected into said circuit, for closing said circuit only when the cover is in the horizontal position.

Claim 7

Dependent Claim 51 words

In the electrophoresis apparatus of claim 5, said circuit including a stationary contact within said chamber connected to one of said electrodes; and a movable contact on and depending from said cover connected to one of said tilt switches, for closing said circuit when said cover is in a horizontal position.

Claim 8

Dependent Claim 55 words

In the electrophoresis apparatus of claim 3, yieldable latches upon said lift and transfer assembly anchoring said cover in closed position when said assembly is in its lowermost retracted position, upward movement of said lift and transfer assembly, tilting said cover upwardly and out of the path of upward movement of said plate carrying rack.

Claim 9

Dependent Claim 34 words

In the electrophoresis apparatus of claim 1, said chamber and each of said vats at their upper ends having an upright lift tab to facilitate loading and unloading thereof into and from said housing.

Claim 10

Dependent Claim 26 words

In the electrophoresis apparatus of claim 1, portions of said rack arms along their undersurfaces being shaped for cooperative registry with said pairs of housing notches.

Claim 11

Dependent Claim 20 words

In the electrophoresis apparatus of claim 1, said electrophoresis plates being flexed for upright frictional retention within said rack frame.

Claim 12

Dependent Claim 114 words

In the electrophoresis apparatus of claim 1, said lift and transfer assembly being a walking beam device including a pair of upright parallel interconnected lift and transfer plates, outward of the housing front and rear walls, and movably mounted thereon, said plates adapted for successive movements upwardly, forwardly, downwardly and rearwardly; said upward movement lifting said plate holder rack from one pair of housing notches, said forward movement transferring said rack to overlie the next adjacent pair of housing notches; said downward movement positioning said rack upon said latter pair of notches, further downward movement disengaging said lift plates from said rack, and said rearward movement repositioning said lift plates to their initial position.

Claim 13

Dependent Claim 125 words

In the electrophoresis apparatus of claim 11, said lift and transfer plates having transversely aligned longitudinally spaced pairs of notches in and along their upper edges; said pairs of notches being in alignment with the corresponding housing pairs of notches respectively; said rack arms overlying said lift and transfer plates adapted for nesting and support within the lift and transfer plate notches upon upward movement of said lift and transfer plates; whereby the respective pairs of notches in said lift and transfer plates successively receive said rack arms as they are progressively transferred to successive pairs of housing notches until the rack and its vertically supported plates have been transferred successively from the electrophoresis chamber, and into each of the respective vats for predetermined intervals.

Claim 14

Dependent Claim 38 words

In the electrophoresis apparatus of claim 12, the housing and lift plate notches being V shaped; portions of said rack arms along their undersurfaces being V-shaped for cooperative registry with said pairs of housing and lift plate notches.

Claim 15

Dependent Claim 68 words

In the electrophoresis apparatus of claim 11, the mounting of said lift and transfer plates upon said housing including transversely opposed pairs of longitudinally spaced lift spocket chains journalled upon and outwardly of said housing adjacent its opposite ends and movable in unison; and corresponding outwardly directed lift pins upon each sprocket chain extending through and journalled within said lift and transfer plates adjacent their lower ends respectively.

Claim 16

Dependent Claim 60 words

In the electrophoresis apparatus of claim 14, the supporting of said sprocket chains including idler sprocket gears journalled upon said housing receiving the upper end of each sprocket chain respectively; drive sprocket gears rotatably journalled upon said housing operatively receiving the lower end of each sprocket chain respectively; and power means upon said housing operatively engaging said drive sprocket gears.

Claim 17

Dependent Claim 85 words

In the electrophoresis apparatus of claim 15, said power means including a motor mounted upon said housing having a drive shaft mounting a sprocket gear; a pair of longitudinally spaced transverse axle shafts journalled upon said housing and projecting from opposite sides thereof; said shafts mounting at their ends the respective drive sprocket gears for said lift sprocket chains; transfer sprocket gears secured upon each axle shaft; and a drive chain interconnecting said transfer sprocket gears and operatively engaged by said motor shaft sprocket gear.

Claim 18

Dependent Claim 65 words

In the electrophoresis apparatus of claim 16, the pin connection between said lift sprocket chains and said lift plates being such that said lift and transfer plates move in unison upwardly, forwardly, downwardly and rearwardly; and an electrical circuit including said motor connected to a power source, and including a pre-set adjustable program timer interrupting movements of said lift and transfer plates for predetermined intervals.

Claim 19

Dependent Claim 37 words

In the electrophoresis apparatus of claim 2, said power means including a fan, an air conduit aligned therewith having an outlet communicating with the base of said drying chamber; and an electric resistance heater in said conduit.

Claim 20

Dependent Claim 49 words

In the electrophoresis apparatus of claim 3, a transverse stop on said cabinet limiting opening movement of said cover; at a stop upon said lift and transfer assembly engageable with said cover when closed, and adapted for opening said cover on upward movement of said lift and transfer assembly.

Claim 21

Dependent Claim 57 words

In the electrophoresis apparatus of claim 1, said electrophoretic circuit including a power source and a voltage switch for selectively producing 180, 300, 350 volts to said circuit; depending upon the nature of the sample, and a +- polarity switch in said circuit for preselecting polarity between said anode and cathode for upward and downward migration respectively.

Claim 22

Dependent Claim 24 words

In the electrophoresis apparatus of claim 11, said lift plates having formed therethrough a series of rows of longitudinally spaced weight reducing cut-outs therein.

Claim 23

Dependent Claim 60 words

In the electrophoresis apparatus of claim 11, there being an upright plate drying chamber within said housing, upon said platform between the last vat and the housing end wall; the connection between said lift plates including an end plate extending between the one ends of said lift plates at their lower ends adapted for registry with said plate drying chamber.

Claim 24

Dependent Claim 24 words

In the electrophoresis apparatus of claim 11, the top of said lift plates when in their lowermost position being substantially enclosed within said cabinet.

Claim 25

Dependent Claim 120 words

In the electrophoresis apparatus of claim 1, said circuit including an electrical power source, a motor for driving said lift and transfer assembly, a timer for pre-setting the length of time that the voltage is applied to said electrophoresis chamber; a control panel on said cabinet having an off and on switch connected into said circuit, a voltage selector connected into said circuit for variably selecting 180, 300 and 350 volts delivered to the electrophoresis chamber; and a time control connected to said timer and variable between 0 and 60 minutes, and a multiple position program switch for presetting the length of time the sample plates are left in each of the stain cycle vats, or for manual control thereof.

Claim 26

Independent Claim 111 words

The method of electrophoresis and staining which includes: supporting in an upright position a non-conductive plate having on one surface an electrophoresis media to which has been applied a sample selected from the group consisting of serum proteins, lipoproteins, hemoglobins and isoenzymes; enclosing said plate within a chamber; applying an electrical potential to the plate ends within an electrical power circuit for a preselected period, and at a preselected voltage the specimen fractionating and migrating laterally over portions of said plate; elevating said plate after electrophoresis; laterally translating said plate while maintaining it in an upright position; and lowering said plate immersing it within a staining solution for a predetermined interval.

Claim 27

Independent Claim 111 words

The method of electrophoresis and staining which includes: supporting in an upright position a non-conductive plate having on one surface an electrophoresis media to which has been applied a sample selected from the group consisting of serum proteins, lipoproteins, hemoglobins and isoenzymes; enclosing said plate within a chamber; applying an electrical potential to the plate ends within an electrical power circuit for a preselected period, and at a preselected voltage the specimen fractionating and migrating laterally over portions of said plate; elevating said plate after electrophoresis; laterally translating said plate while maintaining it in an upright position; and lowering said plate immersing it within a staining solution for a predetermined interval.

Claim 28

Dependent Claim 29 words

In the method of claim 25, said electrophoresis media selected from the group consisting of cellulose acetate, agarose, cellulose esters, acrylamide gel and any other media suitable for electrophoresis.

Claim 29

Dependent Claim 88 words

In the method of claim 25, said chamber having a hinged cover, said application of the electrical potential including electric conductors on the bottom of said chamber and in said cover respectively; and positioning a buffer moistened conductive sponge within a buffer solution within said container to overlie one conductor and a second buffer moistened conductive sponge within said cover engaging the other conductor, the top and bottom edges of said plate being yieldably embedded into the surfaces of said sponges respectively for completing the electrical circuit therethrough.

Claim 30

Dependent Claim 34 words

In the method of claim 25, the supporting of said plate including a rack having a horizontal open frame; said plate being bowed horizontally for frictional retention within said rack in an upright position.

Claim 31

Dependent Claim 26 words

In the electrophoresis apparatus of claim 1, said series of processing solutions including treating solutions 1, 2 and 3, a fixative solution and a rinse solution.

Claim 32

Dependent Claim 16 words

In the electrophoresis apparatus of claim 9, said notches being V-shaped, said rack undersurfaces being V-shaped.

Annuity Stage	Window Opens	Payment Deadline	Surcharge Period Until	Estimated Fee	Status
3.5 Years (Year 4)	1986-07-04	1987-01-03	1987-07-05	$2000.00	Window Closed / Lapsed
7.5 Years (Year 8)	1990-07-04	1991-01-03	1991-07-05	$3760.00	Window Closed / Lapsed
11.5 Years (Year 12)	1994-07-04	1995-01-03	1995-07-05	$7700.00	Window Closed / Lapsed

Automated electrophoresis and staining apparatus and method

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