US4390628A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Process for isolating Cu, Zn-superoxide dismutase from aqueous solutions containing said enzyme together with accompanying proteins
Assignee: De Forenede Bryggerier AS
•
Filing: 1981-11-10
•
Granted: 1983-06-28
•
Expires: 2001-11-10
Abstract
Abstract Cu,Zn-superoxide dismutase (SOD) is isolated from aqueous solutions containing said enzyme together with accompanying proteins by chromatography of the solution at a pH of 4.7 to 5.0 on a cation exchange resin of the same polarity as SOD in the pH range used. As cation exchange resin may particularly be used carboxymethyl celluloses, cross-linked dextrans substituted with carboxymethyl groups or sulfopropyl groups or cross-linked agaroses substituted with carboxymethyl groups. The process lends itself to use on an industrial scale and provides a high yield of pure SOD.
Inventors
- 👤 Jack T. Johansen
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-26 14:47
CPC Classifications
C; C12; C12N; C12N9/00; C12N9/0004; C12N9/0089; C12N9/0065; C12N9/88; Y; Y10; Y10S; Y10S435/00; Y10S435/814; Y10S435/815
Claims Analysis
Total Claims: 4
Claim 1
Independent Claim
47 words
A process for isolating Cu,Zn-superoxide dismutase (S0D) from aqueous solutions containing said enzyme together with accompanying proteins, characterized by subjecting the solution at a pH of 4.7 to 5.0 to chromatography on a cation exchange resin of the same polarity as SOD in the pH range used.
Claim 2
Dependent Claim
47 words
A process for isolating Cu,Zn-superoxide dismutase (S0D) from aqueous solutions containing said enzyme together with accompanying proteins, characterized by subjecting the solution at a pH of 4.7 to 5.0 to chromatography on a cation exchange resin of the same polarity as SOD in the pH range used.
Claim 3
Dependent Claim
32 words
A process according to claim 1, characterized by using as cation exchange resin carboxymethyl celluloses or cross-linked dextrans substituted with carboxymethyl groups or sulfopropyl groups or cross-linked agaroses substituted with carboxymethyl groups.
Claim 4
Dependent Claim
17 words
A process according to claim 1, characterized in that the pH of the solution is about 4.8.
References Cited (Backward Citations)
US3758682A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1986-06-27 | 1986-12-27 | 1987-06-28 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1990-06-27 | 1990-12-27 | 1991-06-28 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1994-06-27 | 1994-12-27 | 1995-06-28 | $7700.00 | Window Closed / Lapsed |