US4386068A
Expired / Lapsed
US
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Antihemophilic factor concentrate and method for preparation
Assignee: Bayer Corp
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Filing: 1980-02-26
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Granted: 1983-05-31
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Expires: 2000-02-26
Abstract
Abstract Novel antihemophilic factor concentrates and process for making the same are disclosed. An aqueous solution of antihemophilic factor proteins is obtained, preferably by solubilizing antihemophilic factor proteins from blood plasma cryoprecipitate in water. The aqueous solution is purified to remove unwanted protein, for example, by mixing the aqueous solution with aluminum hydroxide. Then, the aqueous solution is ultrafiltered to concentrate it, mixed with buffer and saline and adjusted to an acid pH, and freeze-dried.
Inventors
- 👤 Gautam Mitra
- 👤 John L. Lundblad
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-27 06:01
CPC Classifications
C; C07; C07K; C07K14/00; C07K14/435; C07K14/745; C07K14/755; A; A61; A61K; A61K38/00
Claims Analysis
Total Claims: 37
Claim 1
Independent Claim
58 words
A process for the production of antihemophilic factor concentrate, comprising the steps of (a) subjecting an aqueous solution of antihemophilic factor proteins to purification, (b) subjecting the purified aqueous solution of antihemophilic factor of step (a) to ultrafiltration to remove water, and (c) mixing the concentrated aqueous solution of antihemophilic factor of step (b) with buffer and saline.
Claim 2
Dependent Claim
58 words
A process for the production of antihemophilic factor concentrate, comprising the steps of (a) subjecting an aqueous solution of antihemophilic factor proteins to purification, (b) subjecting the purified aqueous solution of antihemophilic factor of step (a) to ultrafiltration to remove water, and (c) mixing the concentrated aqueous solution of antihemophilic factor of step (b) with buffer and saline.
Claim 3
Dependent Claim
19 words
The process of claim 1 which further includes the step of freeze-drying the ultrafiltered aqueous solution of step (c).
Claim 4
Dependent Claim
25 words
The process of claim 1 wherein the aqueous solution of antihemophilic factor proteins is purified in step (a) by mixing with an aluminum hydroxide adsorbent.
Claim 5
Dependent Claim
13 words
The process of claim 3 wherein the pH of the mixture is acidic.
Claim 6
Dependent Claim
25 words
The process of claim 1 wherein the ultrafiltration is conducted using an ultrafiltration membrane having a nominal molecular weight cut-off less than one million daltons.
Claim 7
Dependent Claim
28 words
The process of claim 1 wherein the ultrafiltration is conducted using an ultrafiltration membrane having a nominal molecular weight cut-off within the range of 10,000 to 300,000 daltons.
Claim 8
Dependent Claim
16 words
The process of claim 1 wherein the ultrafiltration is conducted using a hollow fiber ultrafiltration membrane.
Claim 9
Dependent Claim
25 words
The process of claim 1 wherein the ultrafiltration is conducted with a laminar flow rate at the ultrafiltration membrane wall less than Reynolds Number 2000.
Claim 10
Dependent Claim
24 words
The process of claim 1 wherein the ultrafiltration is conducted with a shear rate at the ultrafiltration membrane wall less than 2000 reverse seconds.
Claim 11
Dependent Claim
14 words
The process of claim 1 wherein the ultrafiltration is conducted using a diaphragm pump.
Claim 12
Dependent Claim
22 words
The process of claim 1 which further includes the step of mixing the ultrafiltered aqueous solution with glycine to further purify it.
Claim 13
Dependent Claim
22 words
The process of claim 11 wherein the ultrafiltered aqueous solution is mixed with glycine prior to mixing it with buffer and saline.
Claim 14
Dependent Claim
27 words
The process of claim 1 wherein the aqueous solution of antihemophilic factor proteins is obtained by solubilizing antihemophilic factor proteins from blood plasma cryoprecipitate in aqueous medium.
Claim 15
Dependent Claim
27 words
The process of claim 1 which further includes the step of adjusting the aqueous solution of step (c) to a slightly acid pH by addition of acid.
Claim 16
Dependent Claim
74 words
A process for the production of antihemophilic factor concentrate, comprising the steps of (a) subjecting an aqueous solution of antihemophilic blood plasma cryoprecipitate to purification using an aluminum hydroxide adsorbent, (b) subjecting the purified aqueous solution of antihemophilic factor of step (a) to ultrafiltration to remove water, (c) mixing the concentrated solution of antihemophilic factor of step (b) with buffer and saline and adjusting to an acid pH, and then (d) freeze-drying the mixture.
Claim 17
Dependent Claim
74 words
A process for the production of antihemophilic factor concentrate, comprising the steps of (a) subjecting an aqueous solution of antihemophilic blood plasma cryoprecipitate to purification using an aluminum hydroxide adsorbent, (b) subjecting the purified aqueous solution of antihemophilic factor of step (a) to ultrafiltration to remove water, (c) mixing the concentrated solution of antihemophilic factor of step (b) with buffer and saline and adjusting to an acid pH, and then (d) freeze-drying the mixture.
Claim 18
Dependent Claim
25 words
The process of claim 15 wherein the ultrafiltration is conducted using an ultrafiltration membrane having a nominal molecular weight cut-off less than one million daltons.
Claim 19
Dependent Claim
28 words
The process of claim 15 wherein the ultrafiltration is conducted using an ultrafiltration membrane having a nominal molecular weight cut-off within the range of 10,000 to 300,000 daltons.
Claim 20
Dependent Claim
16 words
The process of claim 15 wherein the ultrafiltration is conducted using a hollow fiber ultrafiltration membrane.
Claim 21
Dependent Claim
25 words
The process of claim 15 wherein the ultrafiltration is conducted with a laminar flow rate at the ultrafiltration membrane wall less than Reynolds Number 2000.
Claim 22
Dependent Claim
24 words
The process of claim 15 wherein the ultrafiltration is conducted with a shear rate at the ultrafiltration membrane wall less than 2000 reverse seconds.
Claim 23
Dependent Claim
14 words
The process of claim 15 wherein the ultrafiltration is conducted using a diaphragm pump.
Claim 24
Dependent Claim
22 words
The process of claim 15 which further includes the step of mixing the ultrafiltered aqueous solution with glycine to further purify it.
Claim 25
Dependent Claim
22 words
The process of claim 22 wherein the ultrafiltered aqueous solution is mixed with glycine prior to mixing it with buffer and saline.
Claim 26
Dependent Claim
94 words
In the process for the production of antihemophilic factor concentrate in purified form wherein an aqueous solution of antihemophilic blood plasma cryoprecipitate is subjected to purification by mixing with an aluminum hydroxide adsorbent and by precipitating unwanted protein in the cold and wherein the aqueous solution containing antihemophilic factor is mixed with buffer and saline and adjusted to an acid pH, and wherein the aqueous solution containing antihemophilic factor is freeze-dried, the improvement which comprises concentrating the aqueous solution containing antihemophilic factor by removal of water prior to mixing it with buffer and saline.
Claim 27
Dependent Claim
94 words
In the process for the production of antihemophilic factor concentrate in purified form wherein an aqueous solution of antihemophilic blood plasma cryoprecipitate is subjected to purification by mixing with an aluminum hydroxide adsorbent and by precipitating unwanted protein in the cold and wherein the aqueous solution containing antihemophilic factor is mixed with buffer and saline and adjusted to an acid pH, and wherein the aqueous solution containing antihemophilic factor is freeze-dried, the improvement which comprises concentrating the aqueous solution containing antihemophilic factor by removal of water prior to mixing it with buffer and saline.
Claim 28
Dependent Claim
17 words
Process according to claim 24, where the water is removed by subjecting the aqueous extract to ultrafiltration.
Claim 29
Dependent Claim
16 words
Process according to claim 25, where the water is removed by means of a semi-permeable membrane.
Claim 30
Dependent Claim
25 words
The process of claim 24 wherein the ultrafiltration is conducted using an ultrafiltration membrane having a nominal molecular weight cut-off less than one million daltons.
Claim 31
Dependent Claim
28 words
The process of claim 24 wherein the ultrafiltration is conducted using an ultrafiltration membrane having a nominal molecular weight cut-off within the range of 10,000 to 300,000 daltons.
Claim 32
Dependent Claim
16 words
The process of claim 24 wherein the ultrafiltration is conducted using a hollow fiber ultrafiltration membrane.
Claim 33
Dependent Claim
25 words
The process of claim 24 wherein the ultrafiltration is conducted with a laminar flow rate at the ultrafiltration membrane wall less than Reynolds Number 2000.
Claim 34
Dependent Claim
24 words
The process of claim 24 wherein the ultrafiltration is conducted with a shear rate at the ultrafiltration membrane wall less than 2000 reverse seconds.
Claim 35
Dependent Claim
14 words
The process of claim 24 wherein the ultrafiltration is conducted using a diaphragm pump.
Claim 36
Dependent Claim
18 words
The process of claim 24 which further includes the step of mixing the ultrafiltered aqueous solution with glycine.
Claim 37
Dependent Claim
22 words
The process of claim 33 wherein the ultrafiltered aqueous solution is mixed with glycine prior to mixing it with buffer and saline.
References Cited (Backward Citations)
US4170639A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1986-05-30 | 1986-11-29 | 1987-05-31 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1990-05-30 | 1990-11-29 | 1991-05-31 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1994-05-30 | 1994-11-29 | 1995-05-31 | $7700.00 | Window Closed / Lapsed |