Assay method of enzyme activity

Abstract

Abstract Novel amine compounds of the formula ##STR1## wherein R 1 is lower alkyl and R 2 is substituted methyl amino or D-amino acid residue, or a salt thereof. R 1 can typically be isobutyl or methyl; and R 2 can be methylamino of the formula --NH--CH.sub.2 --R.sub.3 in which R 3 is organic, more particularly methyl, ethyl, n-propyl, isopropyl, n-butyl, amyl, p-hydroxybenzyl, 3,4-di-hydroxybenzyl, 5-imidazolemethyl, 3-imidazolemethyl or phenyl. R 2 can also be D-amino acid residue of the formula ##STR2## in which one of R 4 and R 5 is carboxyl and the other is organic, more particularly methylthioethyl, isobutyl, methyl or phenyl, and C* is a D-asymmetric carbon atom. The compounds are useful in an assay method for leucine and aminopeptidase which comprises reacting a substrate amide as above, or a salt thereof, with a sample for leucine aminopeptidase assay, incubating the reaction product produced thereby, and measuring the thus-consumed oxygen or liberated hydrogen peroxide.

Inventors

• 👤 Kunio Matsumoto
• 👤 Toshiharu Noda
• 👤 Tadashiro Fujii
• 👤 Susumu Watanabe
• 👤 Kazuhiko Matsumoto
• 👤 Shigeo Katsuragi

Bibliographic Details