US4383905A
Expired / Lapsed
US
⏱️ 0.0 years remaining
System for hydrodynamic compensation for electrophoresis crescent distortion
Assignee: McDonnell Douglas Corp
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Filing: 1981-09-28
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Granted: 1983-05-17
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Expires: 2001-09-28
Abstract
Abstract Crescent distortion of output samples collected during a continuous free flow electrophoresis separation process is reduced by providing means with the electrophoresis chamber whereby lateral flow across the chamber may be controllably varied to introduce beneficial hydrodynamic compensation effects to minimize distortion of the sample streams within the electrophoresis chamber.
Inventors
- 👤 David W. Richman
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-01 12:24
CPC Classifications
G; G01; G01N; G01N27/00; G01N27/26; G01N27/416; G01N27/447; G01N27/44756; G01N27/44769
Claims Analysis
Total Claims: 6
Claim 1
Independent Claim
136 words
In an electrophoresis separation process conducted with an apparatus comprising: a lengthwise elongate rectangular buffer filled separation chamber defined by two elongate spaced apart parallel plates forming a front and a back to the chamber, two sides, an end comprising a collection manifold having a plurality of spaced apart collection tubes, an end comprising a buffer solution inlet manifold assembly; two buffer filled electrode chambers, each disposed adjacent to one of the sides of the chamber and separated therefrom by a porous membrane; at least one sample inlet port located at or near the inlet of the chamber; the improvement comprising controllably varying flow within the apparatus laterally across the chamber such that crescent distortion for at least one of a plurality of individual components of a sample introduced through the sample inlet port is minimized.
Claim 2
Dependent Claim
136 words
In an electrophoresis separation process conducted with an apparatus comprising: a lengthwise elongate rectangular buffer filled separation chamber defined by two elongate spaced apart parallel plates forming a front and a back to the chamber, two sides, an end comprising a collection manifold having a plurality of spaced apart collection tubes, an end comprising a buffer solution inlet manifold assembly; two buffer filled electrode chambers, each disposed adjacent to one of the sides of the chamber and separated therefrom by a porous membrane; at least one sample inlet port located at or near the inlet of the chamber; the improvement comprising controllably varying flow within the apparatus laterally across the chamber such that crescent distortion for at least one of a plurality of individual components of a sample introduced through the sample inlet port is minimized.
Claim 3
Dependent Claim
32 words
The process of claim 1 wherein the flow is controllably varied by inducing a flow from the separation chamber into at least one of the electrode chambers through the corresponding porous membrane.
Claim 4
Dependent Claim
34 words
The process of claim 1 wherein the flow is controllably varied by inducing increased flow out through at least one of the outlet tubes proximate at least one of the sides of the chamber.
Claim 5
Dependent Claim
23 words
The process of claim 1 wherein the flow is controllably varied such that a substantially uniform lateral flow is induced within the chamber.
Claim 6
Dependent Claim
23 words
The process of claim 1 wherein the flow is controllably varied such that a substantially nonuniform lateral flow is induced within the chamber.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1986-05-16 | 1986-11-15 | 1987-05-17 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1990-05-16 | 1990-11-15 | 1991-05-17 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1994-05-16 | 1994-11-15 | 1995-05-17 | $7700.00 | Window Closed / Lapsed |