US4382887A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Novel glycopeptide and methods of isolating and using the same
Assignee: Individual
•
Filing: 1980-07-03
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Granted: 1983-05-10
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Expires: 2000-07-03
Abstract
Abstract A novel glycopeptide is disclosed, which consists of a sugar moiety having a structural formula ##STR1## and a peptide moiety linking to the sugar moiety through N-glycoside bond. The glycopeptide is isolated from organs of normal animals, especially kidney, or urine from healthy human being, and has a biological activity of inducing lesions of kidney in animals. The lesions of kidney induced by an injection of the glycopeptide are very similar to spectrum of human glomerulonephritis. Accordingly, an experimental model for study on human glomerulonephritis is established.
Inventors
- 👤 Seiichi Shibata
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-08 04:01
CPC Classifications
C; C07; C07H; C07H13/00; C07H13/02; C07H13/04; C07H13/06; A; A61; A61K; A61K38/00; Y; Y10; Y10S; Y10S530/00; Y10S530/827; Y10S530/834; Y10S530/835; Y10S530/838; Y10S530/843; Y10S530/846; Y10S530/848; Y10S530/849
Claims Analysis
Total Claims: 8
Claim 1
Independent Claim
139 words
A process of isolating a glycopeptide consisting of a sugar moiety having a structural formula ##STR7## and a peptide moiety linking to the sugar moiety through N-glycoside bond, comprising steps of (1) digesting an organ of normal animal with a protein-digesting enzyme and centrifuging, of (2) digesting the supernatant liquid obtained in the step (1) with pronase and centrifuging, of (3) dialyzing the supernatant liquid obtained in the step (2), of (4) treating the non-dialyzable substance with trichloroacetic acid and centrifuging, and of (5) contacting the supernatant liquid obtained in the step (4) with non-water soluble material having negative charge and eluting the substance adsorbed on the non-water soluble material, wherein said peptide moiety is one obtained from said organ of normal animal which is selected from the group consisting of kidney, lung, aorta, liver, spleen, heart and muscle.
Claim 2
Dependent Claim
139 words
A process of isolating a glycopeptide consisting of a sugar moiety having a structural formula ##STR7## and a peptide moiety linking to the sugar moiety through N-glycoside bond, comprising steps of (1) digesting an organ of normal animal with a protein-digesting enzyme and centrifuging, of (2) digesting the supernatant liquid obtained in the step (1) with pronase and centrifuging, of (3) dialyzing the supernatant liquid obtained in the step (2), of (4) treating the non-dialyzable substance with trichloroacetic acid and centrifuging, and of (5) contacting the supernatant liquid obtained in the step (4) with non-water soluble material having negative charge and eluting the substance adsorbed on the non-water soluble material, wherein said peptide moiety is one obtained from said organ of normal animal which is selected from the group consisting of kidney, lung, aorta, liver, spleen, heart and muscle.
Claim 3
Dependent Claim
33 words
The process claimed in the claim 1 wherein the non-water soluble material having negative charge is a polymeric material containing a functional group having negative charge, or an inorganic material having negative charge.
Claim 4
Dependent Claim
25 words
The process claimed in the claim 2 wherein the polymeric material is the one containing nitrile group, carboxyl group, sulfonic acid group or halogen atom.
Claim 5
Dependent Claim
18 words
The process claimed in the claim 3 wherein the polymeric material is polyvinyl chloride, polyacrylonitrile, cellulose, or cotton.
Claim 6
Dependent Claim
22 words
The process claimed in the claim 2 wherein the inorganic material having negative charge is porous glass, zeolite, silica gel or sellaite.
Claim 7
Dependent Claim
101 words
A process of isolating a glycopeptide consisting of a sugar moiety having a structural formula ##STR8## and a peptide moiety linking to the sugar moiety through N-glycoside bond, comprising steps of (1) contacting urine from healthy human being or the concentrate thereof with non-water soluble material having negative charge and eluting substance absorbed on the non-water soluble material, of (2) digesting the eluate with pronase and centrifuging, of (3) dialyzing the supernatant liquid obtained in the step (3), of (4) treating the non-dialyzable substance with trichloroacetic acid and (5) separating the supernatant containing glycopeptide from the precipitate formed in step (4).
Claim 8
Dependent Claim
101 words
A process of isolating a glycopeptide consisting of a sugar moiety having a structural formula ##STR8## and a peptide moiety linking to the sugar moiety through N-glycoside bond, comprising steps of (1) contacting urine from healthy human being or the concentrate thereof with non-water soluble material having negative charge and eluting substance absorbed on the non-water soluble material, of (2) digesting the eluate with pronase and centrifuging, of (3) dialyzing the supernatant liquid obtained in the step (3), of (4) treating the non-dialyzable substance with trichloroacetic acid and (5) separating the supernatant containing glycopeptide from the precipitate formed in step (4).
References Cited (Backward Citations)
No citation records indexed.
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1986-05-09 | 1986-11-08 | 1987-05-10 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1990-05-09 | 1990-11-08 | 1991-05-10 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1994-05-09 | 1994-11-08 | 1995-05-10 | $7700.00 | Window Closed / Lapsed |