US4379145A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Antitumor protein hybrid and process for the preparation thereof
Assignee: Teijin Ltd
•
Filing: 1980-12-15
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Granted: 1983-04-05
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Expires: 2000-12-15
Abstract
Abstract Antitumor protein hybrid, having a moiety consisting of the antitumor immunoglobulin and a moiety substantially consisting of the fragment A of a diphtheria toxin, which is expressed by the following formula (I): Ab(NHCO--X--S.sub.1 --S.sub.2 --FA).sub.n (I) (where Ab indicates a moiety consisting of the antitumor immunoglobulin and FA indicates a moiety substantially consisting of the fragment A of a diphtheria toxin; X is an alkylene group or phenylene group which either has or does not have a branching of 1 to 5 carbon atoms. N in the amido bond is a nitrogen atom arising from the amino group in the antitumor immunoglobulin; S 1 and S 2 are both sulfur atoms, S 2 indicating a sulfur atom arising from the disulfide bond in a diphtheria toxin; n stands for an integer of 1 to 5). This antitumor protein hybrid has remarkable and specific citotoxicity against tumor cells.
Inventors
- 👤 Yasuhiko Masuho
- 👤 Naoji Umemoto
- 👤 Takeshi Hara
- 👤 Hidematsu Hirai
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-01 03:01
CPC Classifications
C; C07; C07K; C07K16/00; C07K16/18; C07K16/28; C07K16/30; C07K16/303; A; A61; A61K; A61K47/00; A61K47/50; A61K47/51; A61K47/68; A61K47/6801; A61K47/6803; A61K47/6811; A61K47/6817; A61K47/6829; A61K47/6835; A61K47/6881; A61P; A61P35/00; C07K2319/00; C07K2319/55
Claims Analysis
Total Claims: 5
Claim 1
Independent Claim
136 words
Antitumor protein hybrid, having a moiety consisting of the antitumor immunoglobulin and a moiety substantially consisting of the fragment A of a diphtheria toxin, which is expressed by the following formula (I): Ab-(NHCO--X--S.sub.1 --S.sub.2 --FA).sub.n (I) where Ab indicates a moiety consisting of the antitumor immunoglobulin and FA indicates a moiety substantially consisting of the fragment A of a diphtheria toxin; X is an alkylene group or phenylene group which either has or does not have a branch chain of 1 to 5 carbon atoms; N in the amido bond is a nitrogen atom arising from the amino group in the antitumor immunoglobulin; S 1 and S 2 are both sulfur atoms, S 2 indicating a sulfur atom arising from the disulfide bond in a diphtheria toxin; n stands for an integer of 1 to 5.
Claim 2
Dependent Claim
136 words
Antitumor protein hybrid, having a moiety consisting of the antitumor immunoglobulin and a moiety substantially consisting of the fragment A of a diphtheria toxin, which is expressed by the following formula (I): Ab-(NHCO--X--S.sub.1 --S.sub.2 --FA).sub.n (I) where Ab indicates a moiety consisting of the antitumor immunoglobulin and FA indicates a moiety substantially consisting of the fragment A of a diphtheria toxin; X is an alkylene group or phenylene group which either has or does not have a branch chain of 1 to 5 carbon atoms; N in the amido bond is a nitrogen atom arising from the amino group in the antitumor immunoglobulin; S 1 and S 2 are both sulfur atoms, S 2 indicating a sulfur atom arising from the disulfide bond in a diphtheria toxin; n stands for an integer of 1 to 5.
Claim 3
Dependent Claim
13 words
Antitumor protein hybrid according to claim 1, wherein X is an ethylene group.
Claim 4
Dependent Claim
21 words
Antitumor protein hybrid according to claim 1, wherein FA is a moiety consisting of the fragment A of a diphtheria toxin.
Claim 5
Dependent Claim
130 words
A process for the preparation of antitumor protein hybrid expressed by the following formula (I), Ab-NHCO--X--S.sub.1 --S.sub.2 --FA).sub.n (I) where definitions of Ab, FA, X, N, S 1 , S 2 , and n are as same as those given in claim 1, which comprises reacting 1 to 5 amino groups of the antitumor immunoglobulin with a compound which is expressed by the following formula (II), ##STR4## where X is an alkylene group or phenylene group which either has or does not have a branch chain of 1 to 5 carbon atoms, Y represents ##STR5## both S 1 and S 0 indicate a sulfur atom, and reacting the thus obtained product with the substantial fragment A of a diphtheria toxin which has at least one thiol group in the fragment.
References Cited (Backward Citations)
EP0017507A2
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1986-04-04 | 1986-10-04 | 1987-04-05 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1990-04-04 | 1990-10-04 | 1991-04-05 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1994-04-04 | 1994-10-04 | 1995-04-05 | $7700.00 | Window Closed / Lapsed |