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US4376825A Expired / Lapsed US ⏱️ 0.0 years remaining

Enzyme amplification compounds for assays for androgens

Assignee: Siemens Healthcare Diagnostics GmbH Germany
•
Filing: 1980-12-30
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Granted: 1983-03-15
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Expires: 2000-12-30
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Abstract

Abstract Novel biological assay method for determining the presence of a specific organic material by employing a modified enzyme for amplification. By employing receptors specific for one or a group of materials (hereinafter referred to as "ligands") and binding an enzyme to the ligand or ligand counterfeit to provide an "enzyme-bound-ligand", an extremely sensitive method is provided for assaying for ligands. The receptor when bound to the enzyme-bound-ligand substantially inhibits enzymatic activity, providing for different catalytic efficiencies of enzyme-bound-ligand and enzyme-bound-ligand combined with receptor. The receptor, ligand and enzyme-bound-ligand are combined in an arbitrary order and the effect of the presence of ligand on enzymatic activity determined. Various protocols may be used for assaying for enzymatic activity and relating the result to the amount of ligand present.

Inventors

  • 👤 Kenneth E. Rubenstein
  • 👤 Edwin F. Ullman

Bibliographic Details

Application Number —
Jurisdiction US
Data Source google_patents
Last Synchronized 2026-11-03 00:29
CPC Classifications
C; C07; C07J; C07J43/00; C07J43/003; C07J19/00; C07J41/00; C07J41/0005; C07J41/0016; C12; C12N; C12N9/00; C12N9/96; G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/531; G01N33/532; G01N33/535; G01N33/74; G01N33/743; G01N33/94

Claims Analysis

Total Claims: 6
Claim 1
Independent Claim 154 words
An enzyme-bound-ligand of the formula: ##STR61## wherein: any one of the W groups can be --X* or an H of any of the W groups may be replaced by --X*, wherein --X* is a bond or a linking group of from 1 to 8 carbon atoms, and from 1 to 4 heteroatoms; A* is an enzyme bonded at other than its reactive site, having n ligands wherein n is in the range of 1 to the molecular weight of A* divided by 2,000; W 60 is hydrogen or hydroxyl; W 61 is hydrogen, methyl, or hydroxyl; W 62 and W 63 are hydrogen or hydroxyl; with the proviso that at least one of W 60-63 is hydroxy, or two of W 60-63 are taken together to form oxo; W 64 is hydrogen, or two W 64 's are taken together to form a double bond; W 65 is methyl; and W 66 is hydrogen.
Claim 2
Dependent Claim 154 words
An enzyme-bound-ligand of the formula: ##STR61## wherein: any one of the W groups can be --X* or an H of any of the W groups may be replaced by --X*, wherein --X* is a bond or a linking group of from 1 to 8 carbon atoms, and from 1 to 4 heteroatoms; A* is an enzyme bonded at other than its reactive site, having n ligands wherein n is in the range of 1 to the molecular weight of A* divided by 2,000; W 60 is hydrogen or hydroxyl; W 61 is hydrogen, methyl, or hydroxyl; W 62 and W 63 are hydrogen or hydroxyl; with the proviso that at least one of W 60-63 is hydroxy, or two of W 60-63 are taken together to form oxo; W 64 is hydrogen, or two W 64 's are taken together to form a double bond; W 65 is methyl; and W 66 is hydrogen.
Claim 3
Dependent Claim 10 words
An enzyme-bound-ligand according to claim 1, which is an oxidoreductase.
Claim 4
Dependent Claim 10 words
An enzyme-bound-ligand according to claim 1, which is a hydrolase.
Claim 5
Dependent Claim 13 words
An enzyme-bound-ligand according to claim 1, which is testosterone-3-carboxymethyloxime conjugate to malate dehydrogenase.
Claim 6
Dependent Claim 14 words
An enzyme-bound-ligand according to claim 1, which is testosterone-3-carboxymethyloxime conjugate to glucose 6-phosphate dehydrogenase.

References Cited (Backward Citations)

US3850752A Cited
US4191613A Cited

USPTO Annuity & Maintenance Windows

Annuity Stage Window Opens Payment Deadline Surcharge Period Until Estimated Fee Status
3.5 Years (Year 4) 1986-03-14 1986-09-13 1987-03-15 $2000.00 Window Closed / Lapsed
7.5 Years (Year 8) 1990-03-14 1990-09-13 1991-03-15 $3760.00 Window Closed / Lapsed
11.5 Years (Year 12) 1994-03-14 1994-09-13 1995-03-15 $7700.00 Window Closed / Lapsed

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