US4376071A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Mitogenic spinal cord growth factor
Assignee: RESEARCH Corp 405 LEXINGTON AVE NEW YORK 10174 A NEW YORK NOT-FOR-PROFIT CORP
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Filing: 1980-08-21
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Granted: 1983-03-08
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Expires: 2000-08-21
Abstract
Abstract A proteinaceous factor substantially purified from mammalian spinal cord which has mitogenic activity, a molecular weight of about 11,000, a pI of 9.6 and which is heat and acid labile, is useful for the promotion of growth of cells.
Inventors
- 👤 Thomas J. Jennings
- 👤 Allan Lipton
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-25 11:51
CPC Classifications
A; A61; A61K; A61K35/00; A61K35/12; A61K35/28; A61K39/00; Y; Y10; Y10S; Y10S530/00; Y10S530/827; Y10S530/836; Y10S530/839
Claims Analysis
Total Claims: 15
Claim 1
Independent Claim
30 words
A substantially purified proteinaceous factor obtained from spinal cord which has mitogenic activity, a molecular weight of about 11,000, a pI of 9.6 and which is heat and acid labile.
Claim 2
Dependent Claim
30 words
A substantially purified proteinaceous factor obtained from spinal cord which has mitogenic activity, a molecular weight of about 11,000, a pI of 9.6 and which is heat and acid labile.
Claim 3
Dependent Claim
11 words
The factor of claim 1 which is from mammalian spinal cord.
Claim 4
Dependent Claim
11 words
The factor of claim 2 wherein said mammal is a bovid.
Claim 5
Dependent Claim
13 words
The factor of claim 1 which has mitogenic activity towards transformed animal cells.
Claim 6
Dependent Claim
43 words
A process of purifying a mitogenic factor from mammalian spinal cord which comprises: homogenizing mammalian spinal cord; separating said homogenate into a solids fraction and a liquid fraction; fractionating from said liquid fraction molecules which lie in the range of molecular weights 10,000-15,000.
Claim 7
Dependent Claim
43 words
A process of purifying a mitogenic factor from mammalian spinal cord which comprises: homogenizing mammalian spinal cord; separating said homogenate into a solids fraction and a liquid fraction; fractionating from said liquid fraction molecules which lie in the range of molecular weights 10,000-15,000.
Claim 8
Dependent Claim
29 words
The process of claim 5 which after said fractionation step further comprises isolating a proteinaceous mitogenic factor having a molecular weight of about 11,000 and a pI of 9.6.
Claim 9
Dependent Claim
14 words
The process of claim 5 wherein said separation step is carried out by centrifugation.
Claim 10
Dependent Claim
28 words
The process of claim 5 wherein said fractionation step comprises ultrafiltering said liquid fraction, thereby obtaining a residue, and chromatographing said residue on dextran beads crosslinked with epichlorohydrin.
Claim 11
Dependent Claim
18 words
The process of claim 6 wherein said isolation step of said factor is carried out by isoelectric focusing.
Claim 12
Dependent Claim
21 words
A method of promoting the growth of animal of cells which comprises: contacting said cells with the factor of claim 1.
Claim 13
Dependent Claim
11 words
The method of claim 10 wherein said cells are transformed cells.
Claim 14
Dependent Claim
12 words
The method of claim 10 wherein said animal cells are mammalian cells.
Claim 15
Dependent Claim
48 words
A method of preventing the growth of transformed cells which comprises: allowing said transformed cells to bind to a complex of the spinal cord growth factor of claim 1 and an antibody thereto in the presence of complement; thereby causing the complement mediated lysis of said transformed cells.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1986-03-07 | 1986-09-06 | 1987-03-08 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1990-03-07 | 1990-09-06 | 1991-03-08 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1994-03-07 | 1994-09-06 | 1995-03-08 | $7700.00 | Window Closed / Lapsed |