US4356259A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Preserving sperm of domestic animals
Assignee: Individual
•
Filing: 1980-08-15
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Granted: 1982-10-26
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Expires: 2000-08-15
Abstract
Abstract In a method of preserving sperm of domestic animals, especially for the purposes of artificial insemination, a quantity of the sperm, diluted if necessary with a diluting solution, is maintained in communication, through a dialytic membrane, with a quantity of Ringer's solution containing albumin or activated carbon, the whole being maintained at a constant temperature preferably of about 15° C. An antibiotic may be added to the sperm to prevent bacterial growth.
Inventors
- 👤 Kimio Banba
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-27 12:20
CPC Classifications
A; A61; A61K; A61K35/00; A61K35/12; A61K35/48; A61K35/52; A61D; A61D19/00; A61D19/02; A61D19/022
Claims Analysis
Total Claims: 9
Claim 1
Independent Claim
78 words
A method of preserving sperm of domestic animals comprising the steps of: (i) encapsulating a quantity of sperm in a dialytic tube; (ii) placing said dialytic tube in a vessel filled with Ringer's solution containing an absorbent selected from the group consisting of albumin and activated carbon whereby a protective buffer layer of said solution surrounds said dialytic tube, and (iii) maintaining said dialytic tube and said vessel and their contents at a temperature of about 15° C.
Claim 2
Independent Claim
78 words
A method of preserving sperm of domestic animals comprising the steps of: (i) encapsulating a quantity of sperm in a dialytic tube; (ii) placing said dialytic tube in a vessel filled with Ringer's solution containing an absorbent selected from the group consisting of albumin and activated carbon whereby a protective buffer layer of said solution surrounds said dialytic tube, and (iii) maintaining said dialytic tube and said vessel and their contents at a temperature of about 15° C.
Claim 3
Dependent Claim
18 words
The method claimed in claim 1, wherein said sperm is diluted prior to encapsulating in said dialytic tube.
Claim 4
Dependent Claim
21 words
The method claimed in claim 1 wherein the albumin is selected from the group consisting of glair albumin and serum albumin.
Claim 5
Dependent Claim
26 words
The method claimed in claim 1 comprising the step of treating the activated carbon to remove chlorine, prior to placing it in communication with the sperm.
Claim 6
Dependent Claim
16 words
The method claimed in claim 4, wherein the activated carbon is preliminarily washed with hot water.
Claim 7
Dependent Claim
19 words
The method claimed in claim 1 wherein the albumin is in a dialytic solution of 0.5% to 1.0% w/v.
Claim 8
Dependent Claim
20 words
The method claimed in claim 1 wherein the activated carbon is in a dialytic solution of 0.25% to 0.5 w/v.
Claim 9
Dependent Claim
16 words
The method claimed in claim 1 including the step of adding an anti-biotic to the sperm.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1985-10-25 | 1986-04-26 | 1986-10-26 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1989-10-25 | 1990-04-26 | 1990-10-26 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1993-10-25 | 1994-04-26 | 1994-10-26 | $7700.00 | Window Closed / Lapsed |