US4352796A
Expired / Lapsed
US
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Pharmaceutically active plant extract and composition thereof
Assignee: Osaka Chemical Laboratory Co Ltd
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Filing: 1980-06-11
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Granted: 1982-10-05
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Expires: 2000-06-11
Abstract
Abstract There discloses a substantially alkaloid-free aqueous extract of an aconite root which is the dried tuberous root of Aconitum japonicum or like plant belonging to Ranunculaceae, which is useful as analgesic and antiphlogistic.
Inventors
- 👤 Shigeru Arichi
- 👤 Yoshihiro Uchida
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-07 19:59
CPC Classifications
A; A61; A61K; A61K36/00; A61K36/18; A61K36/185; A61K36/71; A61K36/714; A61P; A61P25/00; A61P25/04; A61P29/00
Claims Analysis
Total Claims: 12
Claim 1
Independent Claim
149 words
A substantially alkaloid free aqueous extract of a steamed or powdered aconite root selected from the group consisting of Aconitum japonicum Thunberg or Aconitum sinense Siebold prepared by the process comprising: (a) extracting said aconite root with water, a lower aliphatic alcohol containing water, or a lower aliphatic alcohol to form an extract of said aconite root; (b) concentrating said extract or drying it by evaporation under reduced pressure; (c) dissolving said extract in a slightly basic aqueous solution; (d) treating said aqueous solution with a fat soluble organic solvent selected from the group consisting of chloroform, ethylether, benzene, hexane, toluene, ligroin and petroleum ether until the resulting aqueous solution shows a negative reaction against an alkaloid detecting reagent, thereby removing alkaloids; (e) neutralizing said aqueous solution; and (f) concentrating said aqueous solution as in step (b), whereby a substantially alkaloid-free aqueous extract of said aconite root is obtained.
Claim 2
Dependent Claim
149 words
A substantially alkaloid free aqueous extract of a steamed or powdered aconite root selected from the group consisting of Aconitum japonicum Thunberg or Aconitum sinense Siebold prepared by the process comprising: (a) extracting said aconite root with water, a lower aliphatic alcohol containing water, or a lower aliphatic alcohol to form an extract of said aconite root; (b) concentrating said extract or drying it by evaporation under reduced pressure; (c) dissolving said extract in a slightly basic aqueous solution; (d) treating said aqueous solution with a fat soluble organic solvent selected from the group consisting of chloroform, ethylether, benzene, hexane, toluene, ligroin and petroleum ether until the resulting aqueous solution shows a negative reaction against an alkaloid detecting reagent, thereby removing alkaloids; (e) neutralizing said aqueous solution; and (f) concentrating said aqueous solution as in step (b), whereby a substantially alkaloid-free aqueous extract of said aconite root is obtained.
Claim 3
Dependent Claim
127 words
A substantially alkaloid-free aqueous extract of a steamed or powdered aconite root selected from the group consisting of Aconitum japonicum Thunberg or Aconitum sinense Siebold prepared by the process comprising: (a) impregnating said aconite root with slightly basic water; (b) treating said aconite root with a fat soluble organic solvent selected from the group consisting of chloroform, ethylether, benzene, hexane, toluene, ligroin and petroleum ether until said aconite root shows a negative reaction against an alkaloid detecting reagent, in order to remove alkaloids; (c) extracting said aconite root with water, a lower aliphatic alcohol containing water, or a lower aliphatic alcohol to form an extract of said aconite root; and (d) concentrating said extract, whereby a substantially alkaloid free aqueous extract of said aconite root is obtained.
Claim 4
Dependent Claim
127 words
A substantially alkaloid-free aqueous extract of a steamed or powdered aconite root selected from the group consisting of Aconitum japonicum Thunberg or Aconitum sinense Siebold prepared by the process comprising: (a) impregnating said aconite root with slightly basic water; (b) treating said aconite root with a fat soluble organic solvent selected from the group consisting of chloroform, ethylether, benzene, hexane, toluene, ligroin and petroleum ether until said aconite root shows a negative reaction against an alkaloid detecting reagent, in order to remove alkaloids; (c) extracting said aconite root with water, a lower aliphatic alcohol containing water, or a lower aliphatic alcohol to form an extract of said aconite root; and (d) concentrating said extract, whereby a substantially alkaloid free aqueous extract of said aconite root is obtained.
Claim 5
Dependent Claim
75 words
A substantially alkaloid free aqueous extract of a steamed or powdered aconite root selected from the group consisting of Aconitum japonitum japonicum Thunberg or Aconitum sinense Siebold prepared by the process comprising: (a) extracting said aconite root with water; (b) contacting said extract or concentrate thereof with a cation exchange resin in order to absorb alkaloids thereupon; and (c) concentrating the eluent, whereby a substantially alkaloid free aqueous extract of said aconite root is obtained.
Claim 6
Dependent Claim
75 words
A substantially alkaloid free aqueous extract of a steamed or powdered aconite root selected from the group consisting of Aconitum japonitum japonicum Thunberg or Aconitum sinense Siebold prepared by the process comprising: (a) extracting said aconite root with water; (b) contacting said extract or concentrate thereof with a cation exchange resin in order to absorb alkaloids thereupon; and (c) concentrating the eluent, whereby a substantially alkaloid free aqueous extract of said aconite root is obtained.
Claim 7
Dependent Claim
26 words
The substantially alkaloid free extract according to any of claims 1, 2 or 3, wherein the extract is dryed so as to form a dry extract.
Claim 8
Dependent Claim
29 words
The substantially alkaloid free extract according to either claim 1 or 2, wherein the lower aliphatic alcohol is selected from a group consisting of methanol, propanol, isopropanol and butanol.
Claim 9
Dependent Claim
16 words
The substantially alkaloid free extract according to claim 5, wherein the lower aliphatic alcohol is methanol.
Claim 10
Dependent Claim
20 words
The substantially alkaloid free extract according to either claim 1 or 2, wherein the fat soluble organic solvent is chloroform.
Claim 11
Dependent Claim
21 words
The substantially alkaloid free extract according to claim 3, wherein the cation exchange resin is a strongly acidic cation exchange resin.
Claim 12
Independent Claim
27 words
A method of reducing pain or inflammation in a human being comprising administering an effective amount of the extract of any of claims 1, 2 or 3.
References Cited (Backward Citations)
No citation records indexed.
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| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
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| 3.5 Years (Year 4) | 1985-10-04 | 1986-04-05 | 1986-10-05 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1989-10-04 | 1990-04-05 | 1990-10-05 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1993-10-04 | 1994-04-05 | 1994-10-05 | $7700.00 | Window Closed / Lapsed |