US4351760A
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Novel alkyl substituted fluorescent compounds and polyamino acid conjugates
Assignee: Siemens Healthcare Diagnostics GmbH Germany
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Filing: 1979-09-07
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Granted: 1982-09-28
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Expires: 1999-09-07
Abstract
Abstract Fluorescent antigen conjugates are provided comprising antigens covalently bonded to at least one 2,7-dialiphatic substituted-9-phenyl-6-hydroxy-3H-xanthen-3-one, wherein the 1- and 8-positions are unsubstituted. Also provided are novel fluorescent compounds absorbing at wavelengths in excess of 500 nm, having active functionalities for linking to the antigen. Finally, methods are provided for analyzing antigens in serum, whereby serum interference is avoided.
Inventors
- 👤 Pyare Khanna
- 👤 Edwin F. Ullman
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-28 03:54
CPC Classifications
C; C07; C07J; C07J41/00; C07J41/0033; C07J41/0088; C07D; C07D311/00; C07D311/02; C07D311/78; C07D311/80; C07D311/82; C07J41/0055; G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/531; G01N33/532; G01N33/533; Y; Y10; Y10S; Y10S435/00; Y10S435/961; Y10S435/968; Y10S530/00; Y10S530/806
Claims Analysis
Total Claims: 9
Claim 1
Independent Claim
104 words
A compound of the formula: ##STR9## wherein: E b is hydrogen or chloro; E 2 is chloro; Z 2 is carboxy; R 2 is alkylene of from 1 to 3 carbon atoms; D 2 is hydrogen or carboxy; W 2 is a bond, alkylene, carboxamidoalkylene, or poly(carboxamidoalkylene), wherein alkylene is of from 1 to 2 carbon atoms; Y 2 is non-oxo-carbonyl, carbamoyl, thiocarbamoyl, amino, methylene, oxy or thio; A 2 is a poly(amino acid) ligand or receptor; m 2 is 0 to 3; x 2 is 0 to 4; and n 2 is 1 to the molecular weight of A 2 divided by 500.
Claim 2
Dependent Claim
104 words
A compound of the formula: ##STR9## wherein: E b is hydrogen or chloro; E 2 is chloro; Z 2 is carboxy; R 2 is alkylene of from 1 to 3 carbon atoms; D 2 is hydrogen or carboxy; W 2 is a bond, alkylene, carboxamidoalkylene, or poly(carboxamidoalkylene), wherein alkylene is of from 1 to 2 carbon atoms; Y 2 is non-oxo-carbonyl, carbamoyl, thiocarbamoyl, amino, methylene, oxy or thio; A 2 is a poly(amino acid) ligand or receptor; m 2 is 0 to 3; x 2 is 0 to 4; and n 2 is 1 to the molecular weight of A 2 divided by 500.
Claim 3
Dependent Claim
19 words
A compound according to claim 1, wherein A 2 has a molecular weight of from about 2,000 to 600,000.
Claim 4
Dependent Claim
11 words
A compound according to claim 2, wherein E b is chloro.
Claim 5
Dependent Claim
22 words
A compound according to any one of claims 1 to 3, wherein W 2 is a bond and Y 2 is non-oxo-carbonyl.
Claim 6
Dependent Claim
190 words
A composition of matter consisting of a conjugate bonded to a Support, and of the formula: ##STR10## wherein: n 3 is 1 to the molecular weight of A 2 divided by 500, with the proviso that when q is 0, n 3 is 1; q is 0 or 1; p is at least 1 and up to the molecular weight of Support divided by 500; Support is a molecule of at least about 10,000 molecular weight having a plurality of functionalities for linking; the group in the brackets is bonded by any convenient functionality by a bond or linking group to the Support; E b is hydrogen or chloro; E 2 is chloro; Z 2 is carboxy; R 2 is alkylene of from 1 to 3 carbon atoms; D 2 is hydrogen or carboxy; W 2 is a bond, alkylene, carboxyamidoalkylene or poly(carboxyamidoalkylene), wherein alkylene is of from 1 to 2 carbon atoms; Y 2 is non-oxo-carbonyl, carbamoyl, thiocarbamoyl, amino, methylene, oxy or thio; A 2 is a poly(amino acid) ligand or receptor bonded to Y 2 ; m 2 is 0 to 3. x 2 is 0 to 4.
Claim 7
Dependent Claim
190 words
A composition of matter consisting of a conjugate bonded to a Support, and of the formula: ##STR10## wherein: n 3 is 1 to the molecular weight of A 2 divided by 500, with the proviso that when q is 0, n 3 is 1; q is 0 or 1; p is at least 1 and up to the molecular weight of Support divided by 500; Support is a molecule of at least about 10,000 molecular weight having a plurality of functionalities for linking; the group in the brackets is bonded by any convenient functionality by a bond or linking group to the Support; E b is hydrogen or chloro; E 2 is chloro; Z 2 is carboxy; R 2 is alkylene of from 1 to 3 carbon atoms; D 2 is hydrogen or carboxy; W 2 is a bond, alkylene, carboxyamidoalkylene or poly(carboxyamidoalkylene), wherein alkylene is of from 1 to 2 carbon atoms; Y 2 is non-oxo-carbonyl, carbamoyl, thiocarbamoyl, amino, methylene, oxy or thio; A 2 is a poly(amino acid) ligand or receptor bonded to Y 2 ; m 2 is 0 to 3. x 2 is 0 to 4.
Claim 8
Dependent Claim
14 words
A composition of matter according to claim 5, wherein said support is a polysaccharide.
Claim 9
Dependent Claim
32 words
A composition of matter according to any of claims 5 and 6, wherein A 2 is a poly(amino acid) of from about 2,000 to 600,000 molecular weight and Y 2 is non-oxo-carbonyl.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
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| 3.5 Years (Year 4) | 1985-09-27 | 1986-03-29 | 1986-09-28 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1989-09-27 | 1990-03-29 | 1990-09-28 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1993-09-27 | 1994-03-29 | 1994-09-28 | $7700.00 | Window Closed / Lapsed |