US4347319A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Microbiological epoxidation process
Assignee: ExxonMobil Technology and Engineering Co
•
Filing: 1980-10-24
•
Granted: 1982-08-31
•
Expires: 2000-10-24
Abstract
Abstract A process is disclosed for the microbiological epoxidation of C2-C4 n-alkenes, dienes and vinyl aromatic compounds by contacting, under aerobic conditions, in an aqueous medium containing microorganisms or enzyme preparations derived therefrom, wherein the microorganisms have been aerobically cultivated in a nutrient medium containing methane. The microorganisms used are newly isolated methane-utilizing obligate and facultative methylotrophs.
Inventors
- 👤 Ching-Tsang Hou
- 👤 Ramesh N. Patel
- 👤 Allen I. Laskin
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-08 21:44
CPC Classifications
C; C12; C12N; C12N9/00; C12N9/0004; C12N9/0071; C12N9/0073; C07; C07D; C07D301/00; C07D301/02; C07D303/00; C07D303/02; C07D303/04; C12N1/00; C12N1/26; C12N1/28; C12N1/30; C12N9/0006; C12P; C12P17/00; C12P17/02; C12P7/00; C12P7/02; C12P7/04; C12P7/06; C12P7/24; C12P7/26; C12P7/28; C12Y; C12Y114/00; C12Y114/13; C12Y114/13025; Y; Y02; Y02E; Y02E50/00; Y02E50/10; Y10; Y10S; Y10S435/00; Y10S435/8215; Y10S435/822; Y10S435/858
Claims Analysis
Total Claims: 11
Claim 1
Independent Claim
240 words
A process for the epoxidation of a C 2 -C 4 n-alkene, dienes or vinyl aromatic compounds which comprises: epoxidizing in a reaction medium said alkene, diene or vinyl aromatic compound, under aerobic conditions, in the presence of cells of a bacterial methylotrophic microorganism or any enzyme preparation prepared from said cells and exhibiting oxygenase enzyme activity until at least a portion of said corresponding epoxide is produced in isolable amounts, wherein said microorganisms have been aerobically grown in a nutrient medium containing methane and said microorganism are methane-utilizing micororganism strains selected from the group consisting of: ______________________________________
Methylosinum trichosporium
(CRL 15 PM1)
NRRL B-11,202
Methylosinus sporium
(CRL 16 PM2)
NRRL B-11,203
Methylocystic parvus
(CRL 18 PM4)
NRRL B-11,204
Methylomonas methanica
(CRL M4P) NRRL B-11,205
Methylomonas methanica
(CRL 21 PM7)
NRRL B-11,206
Methylomonas albus
(CRL M8Y) NRRL B-11,207
Methylomonas
streptobacterium (CRL 17 PM3)
NRRL B-11,208
Methylomonas agile
(CRL 22 PM9)
NRRL B-11,209
Methylomonas rubrum
(CRL M6P) NRRL B-11,210
Methylomonas rubrum
(CRL 20 PM6)
NRRL B-11,211
Methylomonas rosaceus
(CRL M10P) NRRL B-11,212
Methylomonas rosaceus
(CRL M7P) NRRL B-11,213
Methylobacter chroococcum
(CRL M6) NRRL B-11,214
Methylobacter chroococcum
(CRL 23, PM8)
NRRL B-11,215
Methylobacter bovis
(CRL M1Y) NRRL B-11,216
Methylobacter bovis
(CRL 19 PM5)
NRRL B-11,217
Methylobacter vinelandii
(CRL M5Y) NRRL B-11,218
Methylococcus capsulatus
(CRL M1) NRRL B-11,219
Methylococcus minimus
(CRL 24 PM12)
NRRL B-11,220
Methylococcus capsulatus
(CRL 25 PM13)
NRRL B-11,221
Methylobacterium
organophilum (CRL 26 R6) NRRL B-11,222
______________________________________ and mutants thereof.
Claim 2
Dependent Claim
240 words
A process for the epoxidation of a C 2 -C 4 n-alkene, dienes or vinyl aromatic compounds which comprises: epoxidizing in a reaction medium said alkene, diene or vinyl aromatic compound, under aerobic conditions, in the presence of cells of a bacterial methylotrophic microorganism or any enzyme preparation prepared from said cells and exhibiting oxygenase enzyme activity until at least a portion of said corresponding epoxide is produced in isolable amounts, wherein said microorganisms have been aerobically grown in a nutrient medium containing methane and said microorganism are methane-utilizing micororganism strains selected from the group consisting of: ______________________________________
Methylosinum trichosporium
(CRL 15 PM1)
NRRL B-11,202
Methylosinus sporium
(CRL 16 PM2)
NRRL B-11,203
Methylocystic parvus
(CRL 18 PM4)
NRRL B-11,204
Methylomonas methanica
(CRL M4P) NRRL B-11,205
Methylomonas methanica
(CRL 21 PM7)
NRRL B-11,206
Methylomonas albus
(CRL M8Y) NRRL B-11,207
Methylomonas
streptobacterium (CRL 17 PM3)
NRRL B-11,208
Methylomonas agile
(CRL 22 PM9)
NRRL B-11,209
Methylomonas rubrum
(CRL M6P) NRRL B-11,210
Methylomonas rubrum
(CRL 20 PM6)
NRRL B-11,211
Methylomonas rosaceus
(CRL M10P) NRRL B-11,212
Methylomonas rosaceus
(CRL M7P) NRRL B-11,213
Methylobacter chroococcum
(CRL M6) NRRL B-11,214
Methylobacter chroococcum
(CRL 23, PM8)
NRRL B-11,215
Methylobacter bovis
(CRL M1Y) NRRL B-11,216
Methylobacter bovis
(CRL 19 PM5)
NRRL B-11,217
Methylobacter vinelandii
(CRL M5Y) NRRL B-11,218
Methylococcus capsulatus
(CRL M1) NRRL B-11,219
Methylococcus minimus
(CRL 24 PM12)
NRRL B-11,220
Methylococcus capsulatus
(CRL 25 PM13)
NRRL B-11,221
Methylobacterium
organophilum (CRL 26 R6) NRRL B-11,222
______________________________________ and mutants thereof.
Claim 3
Dependent Claim
20 words
The process of claim 1 wherein the enzyme preparation is derived from a cell-free particulate fraction of the methane-grown microorganisms.
Claim 4
Dependent Claim
15 words
The process of claim 2 wherein an electron carrier is added to the reaction medium.
Claim 5
Dependent Claim
11 words
The process of claim 3 wherein the electron carrier is NADH.
Claim 6
Dependent Claim
10 words
The process of claim 1 wherein the alkene is propylene.
Claim 7
Dependent Claim
37 words
The process of claim 1 wherein the epoxidation is carried out at a temperature in the range from about 5° to about 55° C. and at a pH in the range from about 4 to about 9.
Claim 8
Dependent Claim
35 words
The process of claim 6 wherein the epoxidation is carried out at a temperature in the range from about 25° to about 50° C. and at a pH in the range from 5.5 to 8.5.
Claim 9
Dependent Claim
12 words
The process of claim 1 wherein the epoxidation is carried out batchwise.
Claim 10
Dependent Claim
21 words
The process of claim 1 wherein the epoxidation is carried out in a batchwise manner and the enzyme preparation is immobilized.
Claim 11
Dependent Claim
21 words
The process of claim 1 wherein the epoxidation is carried out in a continuous manner and the enzyme preparation is immobilized.
References Cited (Backward Citations)
No citation records indexed.
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