US4343901A
Expired / Lapsed
US
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Magnetic support matrix for enzyme immobilization
Assignee: Honeywell UOP LLC
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Filing: 1980-10-22
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Granted: 1982-08-10
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Expires: 2000-10-22
Abstract
Abstract A magnetic support matrix for enzyme immobilization is prepared which comprises a porous, refractory inorganic oxide containing ferromagnetic particles dispersed throughout its interior and a polyamine cross-linked with an excess of a bifunctional reagent impregnated therein so as to furnish pendant functional groups. Such a magnetic support matrix does not otherwise substantially decrease loading of subsequently immobilized enzyme, nor in any other way substantially alter the properties of the immobilized enzyme system when compared to that prepared on a non-magnetic support.
Inventors
- 👤 Louis J. DeFilippi
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-26 10:43
CPC Classifications
C; C12; C12N; C12N11/00; C12N11/14; G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/543; G01N33/54313; G01N33/54326; G01N33/5434; G01N2446/00; G01N2446/20; G01N2446/30; G01N2446/80; G01N2446/86
Claims Analysis
Total Claims: 19
Claim 1
Independent Claim
92 words
A magnetic support matrix comprising a porous, refractory inorganic oxide selected from the group consisting of alumina, thoria, magnesia and combinations thereof, through the interior of which are dispersed particles, from about 0.05 micron to about 0.5 mm in size, of ferromagnetic materials in an amount from about 1 to about 40 percent by weight with less than about 10 percent of said particles on the surface of the oxide, said oxide being impregnated with a polyamine cross-linked with an excess of a bifunctional reagent so as to furnish pendant functional groups.
Claim 2
Dependent Claim
92 words
A magnetic support matrix comprising a porous, refractory inorganic oxide selected from the group consisting of alumina, thoria, magnesia and combinations thereof, through the interior of which are dispersed particles, from about 0.05 micron to about 0.5 mm in size, of ferromagnetic materials in an amount from about 1 to about 40 percent by weight with less than about 10 percent of said particles on the surface of the oxide, said oxide being impregnated with a polyamine cross-linked with an excess of a bifunctional reagent so as to furnish pendant functional groups.
Claim 3
Dependent Claim
11 words
The support matrix of claim 1 wherein the oxide is alumina.
Claim 4
Dependent Claim
27 words
The support matrix of claim 1 where said ferromagnetic materials are selected from the group consisting of iron, cobalt, nickel, magnetite and their ferromagnetic alloys and compounds.
Claim 5
Dependent Claim
23 words
The support matrix of claim 1 wherein the size of the particles of ferromagnetic materials are from about 0.5 to about 50 microns.
Claim 6
Dependent Claim
25 words
The support matrix of claim 1 wherein the polyamine is selected from the group consisting of polyethyleneimine, tetraethylenepentamine, ethylenediamine, diethylenetriamine, triethylenetetramine, pentaethylenehexamine, hexamethylenediamine, and phenylenediamine.
Claim 7
Dependent Claim
11 words
The support matrix of claim 5 wherein the polyamine is polyethyleneimine.
Claim 8
Dependent Claim
22 words
The support matrix of claim 1 wherein the bifunctional reagent is selected from the group consisting of glutaraldehyde, succindialdehyde, terephthaldehyde, and toluenediisocyanate.
Claim 9
Dependent Claim
12 words
The support matrix of claim 7 wherein the bifunctional reagent is glutaraldehyde.
Claim 10
Dependent Claim
98 words
A magnetic immobilized enzyme system comprising a porous, refractory inorganic oxide selected from the group consisting of alumina, thoria, magnesia and combinations thereof through the interior of which are dispersed particles, from about 0.05 micron to about 0.5 mm in size, of ferromagnetic materials in an amount from about 1 to about 40 percent by weight, with less than about 10 percent of said particles on the surface of the oxide, said oxide being impregnated with a polyamine cross-linked with an excess of a bifunctional reagent so as to furnish pendant functional groups, with an enzyme bound thereto.
Claim 11
Dependent Claim
98 words
A magnetic immobilized enzyme system comprising a porous, refractory inorganic oxide selected from the group consisting of alumina, thoria, magnesia and combinations thereof through the interior of which are dispersed particles, from about 0.05 micron to about 0.5 mm in size, of ferromagnetic materials in an amount from about 1 to about 40 percent by weight, with less than about 10 percent of said particles on the surface of the oxide, said oxide being impregnated with a polyamine cross-linked with an excess of a bifunctional reagent so as to furnish pendant functional groups, with an enzyme bound thereto.
Claim 12
Dependent Claim
12 words
The immobilized enzyme system of claim 9 where the oxide is alumina.
Claim 13
Dependent Claim
28 words
The immobilized enzyme system of claim 9 where said ferromagnetic materials are selected from the group consisting of iron, cobalt, nickel, magnetite and their ferromagnetic alloys and compounds.
Claim 14
Dependent Claim
24 words
The immobilized enzyme system of claim 9 wherein the size of the particles of ferromagnetic materials are from about 0.5 to about 50 microns.
Claim 15
Dependent Claim
26 words
The immobilized enzyme system of claim 9 wherein the polyamine is selected from the group consisting of polyethyleneimine, tetraethylenepentamine, ethylenediamine, diethylenetriamine, triethylenetetramine, pentaethylenehexamine, hexamethylenediamine, and phenylenediamine.
Claim 16
Dependent Claim
12 words
The immobilized enzyme system of claim 13 wherein the polyamine is polyethyleneimine.
Claim 17
Dependent Claim
23 words
The immobilized enzyme system of claim 9 wherein the bifunctional reagent is selected from the group consisting of glutaraldehyde, succindialdehyde, terephthaldehyde, and toluenediisocyanate.
Claim 18
Dependent Claim
13 words
The immobilized enzyme system of claim 15 wherein the bifunctional reagent is glutaraldehyde.
Claim 19
Dependent Claim
57 words
The immobilized enzyme system of claim 9 where the enzyme is selected from the group consisting of glucose isomerase, glucoamylase, lactase, cellulase, glucose oxidase, peroxidase, ribonuclease, urease, histidinase, trypsin, papain, hexokinase, chymotrypsin, acylase, invertase, ficin, lysozyme, protease, pepsin, rennin, xylanase, beta amylase, gamma amylase, asparaginase, cholesterol oxidase, alcohol dehydrogenase, amino acid oxidase, collagenase, arginase, catalase, and deoxyribonuclease.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1985-08-09 | 1986-02-08 | 1986-08-10 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1989-08-09 | 1990-02-08 | 1990-08-10 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1993-08-09 | 1994-02-08 | 1994-08-10 | $7700.00 | Window Closed / Lapsed |