US4340581A
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Process for the immunological determination of basal membrane material and new basal membrane suitable therefor
Assignee: Max-Planck-Gesellschaft zur Förderung der Wissenschaften eV
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Filing: 1980-05-30
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Granted: 1982-07-20
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Expires: 2000-05-30
Abstract
Abstract This invention relates to a process for the immunological determination of basal membrane material in body fluids and provides new basal membrane fragments suitable for such determination, and a process for their preparation.
Inventors
- 👤 Rupert Timpl
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 19:06
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/536; G01N33/537; G01N33/539; G01N33/541; G01N33/68; G01N33/6887; A; A61; A61K; A61K39/00; Y; Y10; Y10S; Y10S530/00; Y10S530/806; Y10S530/81; Y10S530/811; Y10S530/827; Y10S530/828; Y10S530/85; Y10S530/851
Claims Analysis
Total Claims: 24
Claim 1
Independent Claim
104 words
Process for the immunological determination of basal membrane material in body fluids, which process comprises incubating a tagged basal membrane antigen selected from tagged laminin, tagged laminin fragment P1 and tagged basal membrane fragment 7S collagen, with a first antibody specific to said antigen in the presence of the body fluid sample to be tested, whereby antigens in the body fluid sample will compete with said tagged antigen for said antibody, separating the antigen-antibody-complex formed, and determining the quantity of tagged antigen contained in the complex or in the separated liquid as a measure of the basal membrane material contained in said body fluid.
Claim 2
Dependent Claim
104 words
Process for the immunological determination of basal membrane material in body fluids, which process comprises incubating a tagged basal membrane antigen selected from tagged laminin, tagged laminin fragment P1 and tagged basal membrane fragment 7S collagen, with a first antibody specific to said antigen in the presence of the body fluid sample to be tested, whereby antigens in the body fluid sample will compete with said tagged antigen for said antibody, separating the antigen-antibody-complex formed, and determining the quantity of tagged antigen contained in the complex or in the separated liquid as a measure of the basal membrane material contained in said body fluid.
Claim 3
Dependent Claim
12 words
Process as claimed in claim 1 wherein the antigen is tagged radioactively.
Claim 4
Dependent Claim
12 words
Process as claimed in claim 1 wherein the antigen is tagged enzymatically.
Claim 5
Dependent Claim
12 words
Process as claimed in claim 1 wherein the antigen is fluorescence tagged.
Claim 6
Dependent Claim
52 words
Process as claimed in claim 1 wherein the first antibody is from an antiserum raised in a suitable animal species and the step of separating the antigen-antibody complex includes adding, a second antibody which second antibody is against the immunoglobulin G of the animal species used for the preparation of the anti-serum.
Claim 7
Dependent Claim
17 words
Process as claimed in claim 1 wherein the antibody or anti-serum is bound to a solid carrier.
Claim 8
Dependent Claim
19 words
Process as claimed in claim 1 wherein said first antibody is in the form of rabbit anti-serum or antibody.
Claim 9
Dependent Claim
50 words
Laminin suitable for the immunological determination of basal membrane material in body fluids, comprising a glyco protein of 12 to 15% by weight carbohydrate content and a molecular weight of 800 to 1,000 k, consisting of two disulfide-linked polypeptide chains of a molecular weight of about 220 to 440 k.
Claim 10
Dependent Claim
50 words
Laminin suitable for the immunological determination of basal membrane material in body fluids, comprising a glyco protein of 12 to 15% by weight carbohydrate content and a molecular weight of 800 to 1,000 k, consisting of two disulfide-linked polypeptide chains of a molecular weight of about 220 to 440 k.
Claim 11
Dependent Claim
44 words
Laminin fragment P1 suitable for the immunological determination of basal membrane material in body fluids, having a molecular weight of 200 to 300 k, a cysteine content of 12 to 14% Mol, and a high degree of affinity for lectin and stability toward collagenase.
Claim 12
Dependent Claim
44 words
Laminin fragment P1 suitable for the immunological determination of basal membrane material in body fluids, having a molecular weight of 200 to 300 k, a cysteine content of 12 to 14% Mol, and a high degree of affinity for lectin and stability toward collagenase.
Claim 13
Dependent Claim
62 words
Basal membrane peptide 7S collagen suitable for the immunological determination of basal membrane material in body fluids, having a molecular weight of 200 to 300 k, a cysteine content of 2 to 5% Mol, and axial ratio of the order of 1:20, a stable triple helix with a melting point in the range of 60° to 70° C., and resistent to collagenase.
Claim 14
Dependent Claim
62 words
Basal membrane peptide 7S collagen suitable for the immunological determination of basal membrane material in body fluids, having a molecular weight of 200 to 300 k, a cysteine content of 2 to 5% Mol, and axial ratio of the order of 1:20, a stable triple helix with a melting point in the range of 60° to 70° C., and resistent to collagenase.
Claim 15
Dependent Claim
61 words
Process for the preparation of the of claim 8 comprising extracting a tissue material containing a basal membrane in the presence of protease inhibitors, first with high salt concentrations, then with 0.4 to 0.6 M NaCl, separating the laminin by salt precipitation from the extract using 0.4 to 0.6 M NaCl, and optionally dissolving the precipitate and subjecting same to chromatography.
Claim 16
Dependent Claim
59 words
Process for the preparation of laminin fragment P1 comprising extracting tissue containing basal membrane with a concentrate of salt solution of 0.4 to 0.6 M NaCl, dialyzing the solution obtained containing the fraction precipitated between 2 M and 4 M NaCl from the dialyzed solution, and subjecting same to the chromatography with a weakly basic cation-exchanger and with lectin.
Claim 17
Dependent Claim
59 words
Process for the preparation of laminin fragment P1 comprising extracting tissue containing basal membrane with a concentrate of salt solution of 0.4 to 0.6 M NaCl, dialyzing the solution obtained containing the fraction precipitated between 2 M and 4 M NaCl from the dialyzed solution, and subjecting same to the chromatography with a weakly basic cation-exchanger and with lectin.
Claim 18
Dependent Claim
48 words
Process for the preparation of laminin as claimed in claim 8 comprising incubating laminin with protease, dialyzing the solution obtained containing the fraction precipitated between 2 M and 4 M NaCl from the dialyzed solution, and subjecting same to chromatography with a weakly basic cation-exchanger and with lectin.
Claim 19
Dependent Claim
82 words
Process for the preparation of basal membrane peptide 7S collagen suitable for the immunological determination of basal membrane material, which process comprises extracting tissue containing basal membrane in the presence of protease inhibitors with concentrated salt solutions and then with 0.4 to 0.6 M NaCl, incubating same with a protease, dialyzing the resulting solution, precipitating the 7S collagen from the dialyzed solution with 2 M NaCl, dissolving the precipitate, incubating same with collagenase, and the dialyzing and subjecting the incubate to chromatography.
Claim 20
Dependent Claim
82 words
Process for the preparation of basal membrane peptide 7S collagen suitable for the immunological determination of basal membrane material, which process comprises extracting tissue containing basal membrane in the presence of protease inhibitors with concentrated salt solutions and then with 0.4 to 0.6 M NaCl, incubating same with a protease, dialyzing the resulting solution, precipitating the 7S collagen from the dialyzed solution with 2 M NaCl, dissolving the precipitate, incubating same with collagenase, and the dialyzing and subjecting the incubate to chromatography.
Claim 21
Dependent Claim
20 words
Process as claimed in claim 11 wherein the tissue material containing a basal membrane is human placenta or mouse EHS-sarcoma.
Claim 22
Dependent Claim
20 words
Process as claimed in claim 12 wherein the tissue material containing a basal membrane is human placenta or mouse EHS-sarcoma.
Claim 23
Dependent Claim
20 words
Process as claimed in claim 13 wherein the tissue material containing a basal membrane is human placenta or mouse EHS-sarcoma.
Claim 24
Dependent Claim
20 words
Process as claimed in claim 14 wherein the tissue material containing a basal membrane is human placenta or mouse EHS-sarcoma.
References Cited (Backward Citations)
No citation records indexed.
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1985-07-19 | 1986-01-18 | 1986-07-20 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1989-07-19 | 1990-01-18 | 1990-07-20 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1993-07-19 | 1994-01-18 | 1994-07-20 | $7700.00 | Window Closed / Lapsed |