US4335215A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Method of growing anchorage-dependent cells
Assignee: Monsanto Co
•
Filing: 1980-08-27
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Granted: 1982-06-15
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Expires: 2000-08-27
Abstract
Abstract Anchorage-dependent cells are grown in agitated microcarrier suspension in which the cells and microcarriers are aggregated by periodically providing a temporary residence of said microcarriers and cells outside the main cell culture reactor agitation zone and in a separate compartment wherein they are subjected to a gentle tumbling action within a confined space having substantially the same environmental conditions as in the main cell culture reactor.
Inventors
- 👤 William R. Tolbert
- 👤 Mary M. Hitt
- 👤 Joseph Feder
- 👤 Richard C. Kimes
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-27 17:22
CPC Classifications
C; C12; C12P; C12P1/00; C07; C07K; C07K14/00; C07K14/435; C07K14/52; C07K14/555; C12M; C12M23/00; C12M23/02; C12M23/14; C12M25/00; C12M25/16; C12M27/00; C12M27/02; C12M33/00; C12M33/22; C12N; C12N1/00; C12N9/00; C12N9/14; C12N9/48; C12N9/50; C12N9/64; C12N9/6421; C12N9/6424; C12N9/6456; C12N9/6462; C12Y; C12Y304/00; C12Y304/21; C12Y304/21073; C12N2531/00; C12R; C12R2001/00; C12R2001/91; Y; Y10; Y10S; Y10S435/00; Y10S435/811
Claims Analysis
Total Claims: 11
Claim 1
Independent Claim
87 words
In a method of growing anchorage-dependent cells in agitated microcarrier suspension culture, the improvement comprising aggregating the microcarriers and cells by periodically providing a temporary residence of said microcarriers and cells outside the main cell culture reactor agitation zone and in a separate compartment wherein they are subjected to a gentle tumbling action within a confined space having substantially the same environmental conditions as in said main cell culture reactor agitation zone and thereafter recycling said microcarriers and cells to said main cell culture reactor agitation zone.
Claim 2
Dependent Claim
87 words
In a method of growing anchorage-dependent cells in agitated microcarrier suspension culture, the improvement comprising aggregating the microcarriers and cells by periodically providing a temporary residence of said microcarriers and cells outside the main cell culture reactor agitation zone and in a separate compartment wherein they are subjected to a gentle tumbling action within a confined space having substantially the same environmental conditions as in said main cell culture reactor agitation zone and thereafter recycling said microcarriers and cells to said main cell culture reactor agitation zone.
Claim 3
Dependent Claim
48 words
The method of claim 1 in which the microcarriers and cells are periodically carried into and allowed to settle in a tumbling zone of confined space by perfusion of the suspension culture medium through said zone and into a satellite vessel under the force of a pressure differential.
Claim 4
Dependent Claim
23 words
The method of claim 2 in which the cell culture medium is recycled from the satellite vessel to the main cell culture reactor.
Claim 5
Dependent Claim
43 words
The method of claim 1 in which the microcarriers and cells are periodically carried into and allowed to settle in a settling chamber having an inverted conical configuration with a narrow opening at the vertex which empties into the main cell culture reactor.
Claim 6
Dependent Claim
47 words
The method of claim 4 in which the microcarriers and cells are periodically carried into and allowed to settle in the settling chamber by the perfusion of the suspension culture medium through said settling chamber and into a satellite vessel under the force of a pressure differential.
Claim 7
Dependent Claim
23 words
The method of claim 5 in which the cell culture medium is recycled from the satellite vessel to the main cell culture reactor.
Claim 8
Dependent Claim
15 words
The method of claim 1 in which the cells are human diploid foreskin fibroblast cells.
Claim 9
Dependent Claim
16 words
The method of claim 7 in which the cells are treated for the production of interferon.
Claim 10
Dependent Claim
17 words
The method of claim 7 in which the cells are treated for the production of plasminogen activator.
Claim 11
Dependent Claim
17 words
The method of claim 7 in which the cells are treated for the production of angiogenic factor.
References Cited (Backward Citations)
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1985-06-14 | 1985-12-14 | 1986-06-15 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1989-06-14 | 1989-12-14 | 1990-06-15 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1993-06-14 | 1993-12-14 | 1994-06-15 | $7700.00 | Window Closed / Lapsed |