US4332783A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Process for immunologic determination tests
Assignee: Siemens Healthcare Diagnostics GmbH Germany
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Filing: 1979-08-15
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Granted: 1982-06-01
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Expires: 1999-08-15
Abstract
Abstract What is disclosed is a method for the immunological determination of an immuno-complex, comprising an antigen and an antibody, in a liquid containing said immuno-complex, which method comprises (a) incubating said liquid containing said immumo-complex with a first reagent bound to a carrier, said first reagent being specific for an antigenic determinant of the antigen in said immuno-complex and being present in an amount sufficient to fix said immuno-complex; (b) separating the carrier from said liquid containing said immuno-complex and incubating it in a solution of a second reagent, said second reagent being specific for an antigenic determinant of the antibody in said immuno-complex and being present in an amount sufficient to fix said immuno-complex; and (c) separating the carrier from said solution of the second reagent and determining the amount of bound or unbound second reagent.
Inventors
- 👤 Walter Pernice
- 👤 Hans-Harald Sedlacek
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 20:38
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/564; Y; Y10; Y10S; Y10S436/00; Y10S436/80; Y10S436/804; Y10S436/828
Claims Analysis
Total Claims: 11
Claim 1
Independent Claim
132 words
A method for the immunological determination of a immuno-complex, comprising an antigen and an antibody, in a liquid containing said immuno-complex, which method comprises (a) incubating said liquid containing said immuno-complex with a first reagent bound to a carrier, said first reagent being specific for an antigenic determinant of the antigen in said immuno-complex and being present in amount sufficient to fix said immuno-complex; (b) separating the carrier from said liquid containing said immuno-complex and incubating it in a solution of a second reagent, said second reagent being specific for an antigenic determinant of the antibody in said immuno-complex and being present in an amount sufficient to fix said immuno-complex; and (c) separating the carrier from said solution of the second reagent and determining the amount of bound or unbound second reagent.
Claim 2
Independent Claim
132 words
A method for the immunological determination of a immuno-complex, comprising an antigen and an antibody, in a liquid containing said immuno-complex, which method comprises (a) incubating said liquid containing said immuno-complex with a first reagent bound to a carrier, said first reagent being specific for an antigenic determinant of the antigen in said immuno-complex and being present in amount sufficient to fix said immuno-complex; (b) separating the carrier from said liquid containing said immuno-complex and incubating it in a solution of a second reagent, said second reagent being specific for an antigenic determinant of the antibody in said immuno-complex and being present in an amount sufficient to fix said immuno-complex; and (c) separating the carrier from said solution of the second reagent and determining the amount of bound or unbound second reagent.
Claim 3
Dependent Claim
20 words
A method as in claim 1 wherein said carrier is a biological particle or a shaped organic or inorganic body.
Claim 4
Dependent Claim
18 words
A method as in claim 1 wherein said first reagent is bound covalently or adsorptively to said carrier.
Claim 5
Dependent Claim
12 words
A method as in claim 1 wherein said second reagent is labelled.
Claim 6
Dependent Claim
20 words
A method as in claim 1 wherein at least one of said first or second reagents is an antibody fragment.
Claim 7
Dependent Claim
16 words
A method as in claim 1 wherein said second reagent is protein A from Staphylococcus aureus.
Claim 8
Dependent Claim
14 words
A method as in claim 1 wherein said second reagent is a complement factor.
Claim 9
Dependent Claim
20 words
A method as in claim 1 wherein said second reagent is an antibody or antibody fragment directed against the complement.
Claim 10
Dependent Claim
27 words
A method as in claim 1 wherein said second reagent is an antibody against the Fc-fragment of immunoglobulin or against immunoglobulin aggregates, or against a rheumatic factor.
Claim 11
Dependent Claim
24 words
A method as in claim 1 wherein said second reagent is labelled with a radioactive substance, an enzyme, a co-enzyme, or a fluorescent substance.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1985-05-31 | 1985-11-30 | 1986-06-01 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1989-05-31 | 1989-11-30 | 1990-06-01 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1993-05-31 | 1993-11-30 | 1994-06-01 | $7700.00 | Window Closed / Lapsed |