US4330623A
Expired / Lapsed
US
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Process for solubilization of gonococcal antigen
Assignee: Merck and Co Inc
•
Filing: 1980-02-19
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Granted: 1982-05-18
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Expires: 2000-02-19
Abstract
Abstract Gonococcal antigens are solubilized by a process involving trypsin-digestion.
Inventors
- 👤 Yashwant D. Karkhanis
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-29 14:03
CPC Classifications
A; A61; A61K; A61K39/00; A61K39/02; A61K39/095; Y; Y10; Y10S; Y10S435/00; Y10S435/8215; Y10S435/822; Y10S435/871
Claims Analysis
Total Claims: 5
Claim 1
Independent Claim
55 words
A process for the solubilization of gonococcal antigens in a physiologically acceptable medium comprising: (a) suspending the antigen in aqueous sodium hydroxide at pH 7-9; (b) completely digesting the suspension together with trypsin at 37° C.; (c) eluting the trypsin digest through an affinity column with water; and (d) diluting the eluate with a medium.
Claim 2
Dependent Claim
55 words
A process for the solubilization of gonococcal antigens in a physiologically acceptable medium comprising: (a) suspending the antigen in aqueous sodium hydroxide at pH 7-9; (b) completely digesting the suspension together with trypsin at 37° C.; (c) eluting the trypsin digest through an affinity column with water; and (d) diluting the eluate with a medium.
Claim 3
Dependent Claim
13 words
The process of claim 1 wherein the pH of step (a) is 7.8.
Claim 4
Dependent Claim
26 words
The process of claim 1 wherein the affinity column is packed with an aqueous suspension of 3% polyacrylamide and 4% agarose coupled with soybean trypsin inhibitor.
Claim 5
Dependent Claim
12 words
The process of claim 1 wherein the medium is aqueous sodium chloride.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1985-05-17 | 1985-11-16 | 1986-05-18 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1989-05-17 | 1989-11-16 | 1990-05-18 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1993-05-17 | 1993-11-16 | 1994-05-18 | $7700.00 | Window Closed / Lapsed |