US4330440A
Expired / Lapsed
US
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Activated matrix and method of activation
Assignee: Development Finance Corp of New Zealand
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Filing: 1980-03-10
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Granted: 1982-05-18
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Expires: 2000-03-10
Abstract
Abstract The invention relates to the carbonylation of cross linked substituted and unsubstituted polysaccharides, their copolymers with macroporous synthetic polymers, macroporous synthetic polymers and rigid supports with pendant hydroxyalkyl groups. The carbonylated product can be used to prepare an affinity chromatography matrix which remains an uncharged species at varying pH's. The carbonylated product is also useful for the preparation of other compounds: Typical polysaccharides are agarose, starch, dextran, cellulose and regenerated cellulose, typical macroporous synthetic polymers are acrylamides, acrylates and methacrylates, typical rigid supports are silica beads coated with hydroxy alkyl groups and typical cabonylating agents are N,N'-carbonyl diimidazole; N,N'carbonyl di-1,2,3-benzotriazole; and N,N'-carbonyl di-1,2,4-triazole.
Inventors
- 👤 John S. Ayers
- 👤 Geoffrey S. Bethell
- 👤 William S. Hancock
- 👤 Milton T. W. Hearn
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-30 16:31
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/543; G01N33/544; G01N33/548; C; C08; C08B; C08B15/00; C08B15/05; C08B15/06; C08B37/00; C08B37/0006; C08B37/0036; C08B37/0039; C12; C12N; C12N11/00; C12N11/02; C12N11/10; G01N33/54353
Claims Analysis
Total Claims: 16
Claim 1
Independent Claim
78 words
A compound of the general formula III ##STR8## wherein MATRIX is a water and organic insoluble radical selected from the group consisting of: cross-linked polysaccharides, subsituted by C 2 -C 8 alkyl or alkenyl groups, macroporous synthetic polymers, co-polymers of cross-linked substituted polysaccharides with macroporous synthetic polymers, cross-linked co-polymers of unsubstituted polysaccharides with macroporous synthetic polymers, and rigid supports with pendant hydroxyalkyl groups, and Y is a member selected from the group consisting of: imidazolyl; 1,2,4-triazolyl; and 1,2,3-benzotriazolyl.
Claim 2
Dependent Claim
78 words
A compound of the general formula III ##STR8## wherein MATRIX is a water and organic insoluble radical selected from the group consisting of: cross-linked polysaccharides, subsituted by C 2 -C 8 alkyl or alkenyl groups, macroporous synthetic polymers, co-polymers of cross-linked substituted polysaccharides with macroporous synthetic polymers, cross-linked co-polymers of unsubstituted polysaccharides with macroporous synthetic polymers, and rigid supports with pendant hydroxyalkyl groups, and Y is a member selected from the group consisting of: imidazolyl; 1,2,4-triazolyl; and 1,2,3-benzotriazolyl.
Claim 3
Dependent Claim
41 words
A compound as claimed in claim 1 wherein said MATRIX is a polysaccharide selected from the group consisting of agarose, starch, dextran, cellulose, regenerated cellulose, hydroxy C 2 -C 4 alkylated cellulose and hydroxy C 2 -C 4 alkylated regenerated cellulose.
Claim 4
Dependent Claim
18 words
A compound as claimed in claim 2 wherein said MATRIX is cross-linked allyl dextran and Y is imidazolyl.
Claim 5
Dependent Claim
50 words
The compound as claimed in claim 1 wherein said MATRIX is a copolymer of polyacrylamide with a cross-linked substituted or unsubstituted polysaccharide selected from the group consisting of: agarose, starch, dextran, cellulose, regenerated cellulose, hydroxy C 2 -C 4 alkylated cellulose and hydroxy C 2 -C 4 alkylated regenerated cellulose.
Claim 6
Dependent Claim
16 words
The compound of claim 4 wherein said MATRIX is an agarose/polyacrylamide copolymer and Y is imidazolyl.
Claim 7
Dependent Claim
24 words
The compound of claim 1 wherein said MATRIX is a said rigid support comprising silica beads having pendant ##STR9## radicals and Y is imidazolyl.
Claim 8
Dependent Claim
15 words
The compound of claim 1 wherein said MATRIX is hydroxyethyl methacrylate and Y is imidazolyl.
Claim 9
Dependent Claim
77 words
A process for the preparation of a compound of the general formula III as claimed in claim 1 which process comprises carbonylating a said MATRIX having reactive hydroxyl groups in an organic solvent at a temperature of 0° C. to 80° C., the upper temperature limit being the degradation temperature of said MATRIX, with a carbonylating agent of the general formula IV: ##STR10## wherein Y is selected from the group consisting of an imidazolyl, 1,2,3-triazolyl, and 1,2,3-benzotriazolyl.
Claim 10
Dependent Claim
23 words
The process of claim 8 wherein said organic solvent is a member selected from the group consisting of dioxan, dimethyl formamide and acetone.
Claim 11
Dependent Claim
49 words
The process as claimed in claim 9 wherein said MATRIX is selected from the group consisting of agarose, starch, dextran, cellulose, regenerated cellulose, hydroxy C 2 -C 4 alkylated cellulose and hydroxy C 2 -C 4 alkylated regenerated cellulose substituted by C 2 -C 8 alkyl or alkenyl groups.
Claim 12
Dependent Claim
18 words
A process as claimed in claim 10 wherein said MATRIX is cross-linked allyl dextran and Y is imidazolyl.
Claim 13
Dependent Claim
50 words
The process as claimed in claim 9 wherein said MATRIX is a copolymer of polyacrylamide with a cross-linked substituted or unsubstituted polysaccharide selected from the group consisting of: agarose, starch, dextran, cellulose, regenerated cellulose, hydroxy C 2 -C 4 alkylated cellulose and hydroxy C 2 -C 4 alkylated regenerated cellulose.
Claim 14
Dependent Claim
18 words
The process as claimed in claim 12 where said MATRIX is an agarose/polyacrylamide copolymer and Y is imidazolyl.
Claim 15
Dependent Claim
26 words
The process as claimed in claim 9 wherein said MATRIX is a said rigid support comprising silica beads with pendant ##STR11## radicals and Y is imidazolyl.
Claim 16
Dependent Claim
17 words
The process as claimed in claim 9 wherein said MATRIX is hydroxyethyl methacrylate and Y is imidazolyl.
References Cited (Backward Citations)
US4211865A
Cited
US4224439A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1985-05-17 | 1985-11-16 | 1986-05-18 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1989-05-17 | 1989-11-16 | 1990-05-18 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1993-05-17 | 1993-11-16 | 1994-05-18 | $7700.00 | Window Closed / Lapsed |