US4325867A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Process for the recovery of α-2-SB-glycoprotein
Assignee: Roche Diagnostics GmbH
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Filing: 1980-11-20
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Granted: 1982-04-20
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Expires: 2000-11-20
Abstract
Abstract The present invention provides a process for obtaining α-2-SB-glycoprotein from physiological solutions thereof by binding to a bindable protein immobilized on an insoluble carrier, wherein the carrier-fixed protein is subsequently washed with a buffer solution of pH 6 to 8.5 and thereafter the α-2-SB-glycoprotein is eluted with a buffer solution with a pH of 9 or more and isolated from the eluate.
Inventors
- 👤 Walter Eberle
- 👤 Winfried Albert
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-24 17:07
CPC Classifications
C; C07; C07K; C07K14/00; C07K14/435; C07K14/78; A; A61; A61K; A61K35/00; A61K35/12; A61K35/14; A61K35/16; A61P; A61P7/00; A61P7/04; Y; Y10; Y10S; Y10S530/00; Y10S530/827; Y10S530/829; Y10S530/83
Claims Analysis
Total Claims: 13
Claim 1
Independent Claim
55 words
Process for obtaining alpha-2-SB-glycoprotein from physiological solutions thereof, which process comprises binding the alpha-2-SB-glycoprotein to a bindable protein immobilized on an insoluble carrier, washing the carrier-bound protein with a buffer solution of pH 6 to 8.5, and eluting the alpha-2-SB-glycoprotein with a buffer solution of pH 9 or more, and isolating same from the eluate.
Claim 2
Dependent Claim
55 words
Process for obtaining alpha-2-SB-glycoprotein from physiological solutions thereof, which process comprises binding the alpha-2-SB-glycoprotein to a bindable protein immobilized on an insoluble carrier, washing the carrier-bound protein with a buffer solution of pH 6 to 8.5, and eluting the alpha-2-SB-glycoprotein with a buffer solution of pH 9 or more, and isolating same from the eluate.
Claim 3
Dependent Claim
13 words
Process as claimed in claim 1 wherein the bindable protein used is gelatin.
Claim 4
Dependent Claim
13 words
Process as claimed in claim 1 wherein the bindable protein used is collagen.
Claim 5
Dependent Claim
13 words
Process as claimed in claim 1 wherein the bindable protein used is fibrin.
Claim 6
Dependent Claim
14 words
Process as claimed in claim 1 wherein said carrier is based on a carbohydrate.
Claim 7
Dependent Claim
16 words
Process as claimed in claim 1 wherein the bindable protein is covalently fixed onto said carrier.
Claim 8
Dependent Claim
25 words
Process as claimed in claim 1 wherein the carrier-bound protein is washed with 0.01 to 0.3 moles per liter buffer of pH 7 to 8.
Claim 9
Dependent Claim
19 words
Process as claimed in claim 7 wherein said buffer contains 0.5 to 2 moles per liter of neutral salt.
Claim 10
Dependent Claim
15 words
Process as claimed in claim 8 wherein the neutral salt is an alkali metal chloride.
Claim 11
Dependent Claim
21 words
Process as claimed in claim 1 wherein elution is carried out with a buffer with a pH of 10 to 12.
Claim 12
Dependent Claim
16 words
Process as claimed in claim 1 wherein elution is carried out with a volatile buffer substance.
Claim 13
Dependent Claim
13 words
Process as claimed in claim 11 wherein the eluate obtained is directly lyophilized.
References Cited (Backward Citations)
US4104266A
Cited
US4278594A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1985-04-19 | 1985-10-19 | 1986-04-20 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1989-04-19 | 1989-10-19 | 1990-04-20 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1993-04-19 | 1993-10-19 | 1994-04-20 | $7700.00 | Window Closed / Lapsed |