US4319271A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Automated plate reader
Assignee: Merck and Co Inc
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Filing: 1978-12-18
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Granted: 1982-03-09
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Expires: 1998-12-18
Abstract
Abstract A method for determining the end point in a viral assay comprising electronically scanning a microtiter plate to obtain a plurality of data points from each well, transmitting the data points to a digital computer programed to analyze the data points from each well to determine whether each well is positive or negative for CPE in accordance with predetermined parameters, and calculating the viral titer from the positive or negative results for each well.
Inventors
- 👤 William M. Hurni
- 👤 William J. McAleer
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-25 09:52
CPC Classifications
C; C12; C12Q; C12Q1/00; C12Q1/70; G; G01; G01N; G01N21/00; G01N21/17; G01N21/25; G01N21/251; G01N21/253
Claims Analysis
Total Claims: 5
Claim 1
Independent Claim
73 words
A method for determining viral titer comprising electronically scanning a multi-well assay plate to obtain a plurality of data points from each well, transmitting the data points to a digital computer adapted to analyze the data points from each well and adapted to determine in accordance with predetermined parameters whether each well is positive or negative for cytopathologic effect, and calculating the viral titer from the positive or negative determination for each well.
Claim 2
Independent Claim
73 words
A method for determining viral titer comprising electronically scanning a multi-well assay plate to obtain a plurality of data points from each well, transmitting the data points to a digital computer adapted to analyze the data points from each well and adapted to determine in accordance with predetermined parameters whether each well is positive or negative for cytopathologic effect, and calculating the viral titer from the positive or negative determination for each well.
Claim 3
Dependent Claim
17 words
A method according to claim 1 wherein the calculation of viral titer is performed by the computer.
Claim 4
Dependent Claim
17 words
A method according to claim 1 where the electronic scanning is at about 213 lines per cm.
Claim 5
Dependent Claim
21 words
A method according to claim 1 where the electronic scanning generates about 45,000 data points per cm 2 of surface area.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1985-03-08 | 1985-09-07 | 1986-03-09 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1989-03-08 | 1989-09-07 | 1990-03-09 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1993-03-08 | 1993-09-07 | 1994-03-09 | $7700.00 | Window Closed / Lapsed |