US4318984A
Expired / Lapsed
US
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Stabilized composition, test device, and method for detecting the presence of a sugar in a test sample
Assignee: Bayer Corp
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Filing: 1979-11-13
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Granted: 1982-03-09
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Expires: 1999-11-13
Abstract
Abstract An improved composition, test device and method for detecting the presence of a sugar in a test sample are disclosed. The composition comprises a sugar oxidase, a peroxidase, a chromogen compound capable of producing a detectable response in the presence of hydrogen peroxide and peroxidase, and an enhancer compound having the structure <IMAGE> in which the R substituents, same or different, are hydrogen, lower alkyl, lower alkyloxy, aryl or aryloxy; or in which two of the R substituents bound to a common atom together form a closed ring having 3 to about 6 atoms, said ring being saturated, unsaturated or aromatic. R' is hydrogen or an alkyl or alkenyl group having 1 to about 20 carbon atoms. The composition can be incorporated with a carrier matrix to form a test device. The method comprises contacting a test solution with the device and observing any detectable response.
Inventors
- 👤 Thomas A. Magers
- 👤 David L. Tabb
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-05 10:55
CPC Classifications
C; C12; C12Q; C12Q1/00; C12Q1/54; C12Q1/26; C12Q1/28; C12Q2326/00; C12Q2326/10; C12Q2326/14
Claims Analysis
Total Claims: 33
Claim 1
Independent Claim
143 words
In a composition for detecting the presence of a sugar in a test sample, wherein said composition comprises a peroxidase, a sugar oxidase and a chromogen compound having the structure ##STR5## in which R and R*, same or different, are H, lower alkyl, lower alkyloxy, aryl, or aryloxy, or in which the substituents R* together comprise --CH 2 -- n in which n is 1 or 2; the improvement wherein said composition further comprises an enhancer compound having the structure ##STR6## in which the R substituents, same or different, are as described above; or in which two of said R substituents bound to a common atom together form a closed ring having 3 to about 6 atoms, said ring being saturated, unsaturated, or aromatic; and in which R' is hydrogen or an alkyl or alkenyl group having 1 to about 20 carbon atoms.
Claim 2
Dependent Claim
143 words
In a composition for detecting the presence of a sugar in a test sample, wherein said composition comprises a peroxidase, a sugar oxidase and a chromogen compound having the structure ##STR5## in which R and R*, same or different, are H, lower alkyl, lower alkyloxy, aryl, or aryloxy, or in which the substituents R* together comprise --CH 2 -- n in which n is 1 or 2; the improvement wherein said composition further comprises an enhancer compound having the structure ##STR6## in which the R substituents, same or different, are as described above; or in which two of said R substituents bound to a common atom together form a closed ring having 3 to about 6 atoms, said ring being saturated, unsaturated, or aromatic; and in which R' is hydrogen or an alkyl or alkenyl group having 1 to about 20 carbon atoms.
Claim 3
Dependent Claim
14 words
The improved composition of claim 1 wherein said enhancer compound is R 2 S→O.
Claim 4
Dependent Claim
12 words
The improved composition of claim 1 wherein said enhancer compound is ##STR7##
Claim 5
Dependent Claim
12 words
The improved composition of claim 1 wherein said enhancer compound is ##STR8##
Claim 6
Dependent Claim
14 words
The improved composition of claim 1 wherein said enhancer compound is R 3 N→O.
Claim 7
Dependent Claim
34 words
The improved composition of claim 1 wherein said enhancer compound is dimethyl sulfone, dimethyl sulfoxide, pentamethylene sulfoxide, formamide, N,N-dimethyl formamide, acetamide, N,N-dimethylacetamide, N-N-di(β-hydroxyethyl) acetamide, cocoyldiethanolamide, talloyldiethanolamide, tallowyldiethanolamide, trimethylamine oxide, pyridine-1-oxide, 3-hydroxypyridine-1-oxide, 4-methylpyridine-1-oxide, or 2-chloropyridine-1-oxide.
Claim 8
Dependent Claim
22 words
The improved composition of any of claims 1-5 or 6 wherein said sugar is glucose and said sugar oxidase is glucose oxidase.
Claim 9
Dependent Claim
16 words
The improved composition of any of claims 1-5 or 6 wherein said chromogen compound is o-tolidine.
Claim 10
Dependent Claim
16 words
The improved composition of any of claims 1-5 or 6 wherein said chromogen compound is tetramethylbenzidine.
Claim 11
Dependent Claim
16 words
The improved composition of any of claims 1-5 or 6 wherein said chromogen compound is 2,7-diaminofluorene.
Claim 12
Dependent Claim
153 words
In a test device for detecting the presence of a sugar in a test sample, wherein said device comprises a carrier matrix incorporated with a peroxidase, a sugar oxidase and a chromogen compound having the structure ##STR9## in which R and R*, same or different, are hydrogen, lower alkyl, lower alkyloxy, aryl, or aryloxy, or in which the substituents R* together comprise --CH 2 -- n in which n is 1 or 2; the improvement wherein said carrier matrix is additionally incorporated with an enhancer compound having the structure ##STR10## in which the R substituents, same or different, are as recited above; or in which two of said R substituents bound to a common atom together form a closed ring having 3 to about 6 atoms, said ring being saturated, unsaturated, or aromatic, and in which R' is hydrogen or an alkyl or alkenyl group having from 1 to about 20 carbon atoms.
Claim 13
Dependent Claim
153 words
In a test device for detecting the presence of a sugar in a test sample, wherein said device comprises a carrier matrix incorporated with a peroxidase, a sugar oxidase and a chromogen compound having the structure ##STR9## in which R and R*, same or different, are hydrogen, lower alkyl, lower alkyloxy, aryl, or aryloxy, or in which the substituents R* together comprise --CH 2 -- n in which n is 1 or 2; the improvement wherein said carrier matrix is additionally incorporated with an enhancer compound having the structure ##STR10## in which the R substituents, same or different, are as recited above; or in which two of said R substituents bound to a common atom together form a closed ring having 3 to about 6 atoms, said ring being saturated, unsaturated, or aromatic, and in which R' is hydrogen or an alkyl or alkenyl group having from 1 to about 20 carbon atoms.
Claim 14
Dependent Claim
15 words
The improved test device of claim 11 wherein said enhancer compound is R 2 S→O.
Claim 15
Dependent Claim
13 words
The improved test device of claim 11 wherein said enhancer compound is ##STR11##
Claim 16
Dependent Claim
13 words
The improved test device of claim 11 wherein said enhancer compound is ##STR12##
Claim 17
Dependent Claim
15 words
The improved test device of claim 11 wherein said enhancer compound is R 3 N→O.
Claim 18
Dependent Claim
35 words
The improved test device of claim 11 wherein said enhancer compound is dimethyl sulfone, dimethyl sulfoxide, pentamethylene sulfoxide, formamide, N,N-dimethyl formamide, acetamide, N,N-dimethylacetamide, N,N-di(β-hydroxyethyl) acetamide, cocoyldiethanolamide, talloyldiethanolamide, tallowyldiethanolamide, trimethylamine oxide, pyridine-1-oxide, 3-hydroxypyridine-1-oxide, 4methylpyridine-1-oxide, or 2-chloropyridine-1-oxide.
Claim 19
Dependent Claim
23 words
The improved test device of any of claims 11-15 or 16 wherein said sugar is glucose and said sugar oxidase is glucose oxidase.
Claim 20
Dependent Claim
17 words
The improved test device of any of claims 11-15 or 16 wherein said chromogen compound is o-tolidine.
Claim 21
Dependent Claim
17 words
The improved test device of any of claims 11-15 or 16 wherein said chromogen compound is tetramethylbenzidine.
Claim 22
Dependent Claim
17 words
The improved test device of any of claims 11-15 or 16 wherein said chromogen compound is 2,7-diaminofluorene.
Claim 23
Independent Claim
160 words
A method for detecting the presence of a sugar in a test sample, said method comprising contacting said sample with a test device comprising a carrier matrix incorporated with a peroxidase, a sugar oxidase, a chromogen compound having the structure ##STR13## in which the substituents R and R*, same or different, are hydrogen, lower alkyl, lower alkyloxy, aryl, or aryloxy, or in which the R* substituents together comprise --CH 2 -- n in which n is 1 or 2; and an enhancer compound having the structure ##STR14## in which the R substituents, same or different, are as recited above, or in which two of said R substituents bound to a common atom together form a closed ring, having 3 to about 6 atoms, said ring being saturated, unsaturated, or aromatic; and in which R' is hydrogen or an alkyl or alkenyl group having from 1 to about 20 carbon atoms; and observing a detectable response produced by said test device.
Claim 24
Independent Claim
160 words
A method for detecting the presence of a sugar in a test sample, said method comprising contacting said sample with a test device comprising a carrier matrix incorporated with a peroxidase, a sugar oxidase, a chromogen compound having the structure ##STR13## in which the substituents R and R*, same or different, are hydrogen, lower alkyl, lower alkyloxy, aryl, or aryloxy, or in which the R* substituents together comprise --CH 2 -- n in which n is 1 or 2; and an enhancer compound having the structure ##STR14## in which the R substituents, same or different, are as recited above, or in which two of said R substituents bound to a common atom together form a closed ring, having 3 to about 6 atoms, said ring being saturated, unsaturated, or aromatic; and in which R' is hydrogen or an alkyl or alkenyl group having from 1 to about 20 carbon atoms; and observing a detectable response produced by said test device.
Claim 25
Dependent Claim
13 words
The method of claim 21 wherein said enhancer compound is R 2 S→O.
Claim 26
Dependent Claim
11 words
The method of claim 21 wherein said enhancer compound is ##STR15##
Claim 27
Dependent Claim
11 words
The method of claim 21 wherein said enhancer compound is ##STR16##
Claim 28
Dependent Claim
13 words
The method of claim 21 wherein said enhancer compound is R 3 N→0.
Claim 29
Dependent Claim
33 words
The method of claim 21 wherein said enhancer compound is dimethyl sulfone, dimethyl sulfoxide, pentamethylene sulfoxide, formamide, N,N-dimethyl formamide, acetamide, N,N-dimethylacetamide, N,N-di(β-hydroxyethyl) acetamide, cocoyldiethanolamide, talloyldiethanolamide, tallowyldiethanolamide, trimethylamine oxide, pyridine-1-oxide, 3-hydroxypyridine-1-oxide, 4-methylpyridine-1-oxide, or 2-chloropyridine-1-oxide.
Claim 30
Independent Claim
21 words
The method of any of claims 21-25 or 26 wherein said sugar is glucose and said sugar oxidase is glucose oxidase.
Claim 31
Independent Claim
15 words
The method of any of claims 21-25 or 26 wherein said chromogen compound is o-tolidine.
Claim 32
Independent Claim
15 words
The method of any of claims 21-25 or 26 wherein said chromogen compound is tetramethylbenzidine.
Claim 33
Independent Claim
15 words
The method of any of claims 21-25 or 26 wherein said chromogen compound is 2,7-diaminofluorene.
References Cited (Backward Citations)
US2848308A
Cited
US4071317A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1985-03-08 | 1985-09-07 | 1986-03-09 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1989-03-08 | 1989-09-07 | 1990-03-09 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1993-03-08 | 1993-09-07 | 1994-03-09 | $7700.00 | Window Closed / Lapsed |