US4318784A
Expired / Lapsed
US
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Enzymatic processes
Assignee: National Research Development Corp UK
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Filing: 1980-04-15
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Granted: 1982-03-09
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Expires: 2000-04-15
Abstract
Abstract There are provided processes for carrying out enzymatic reactions, especially processes for the oxidative or reductive transformation of organic compounds catalyzed by enzymes, in which the enzymes require a continuing supply of reducing equivalents to regenerate reduced enzyme species in enzymatically active form, and in which the reducing equivalents are derived electrochemically, including electrochemically from molecular hydrogen, the reducing equivalents preferably being passed directly to the enzyme from the cathode of an electrochemical system.
Inventors
- 👤 Irving J. Higgins
- 👤 Hugh A. O. Hill
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-29 04:56
CPC Classifications
C; C12; C12P; C12P7/00; C12P7/02; C12P7/04; C12M; C12M21/00; C12M21/18; C12M35/00; C12M35/08; C12P1/00; C12P7/24
Claims Analysis
Total Claims: 15
Claim 1
Independent Claim
55 words
A process for carrying out an enzymatic reaction, which comprises: continuously supplying reducing equivalents to regenerate a reduce enzyme species in enzymatically active form, said reducing equivalents being derived electrochemically and passed directly to said enzyme from the cathode of an electrochemical system and taken-up and utilized by an electron receptor component of said enzyme.
Claim 2
Dependent Claim
55 words
A process for carrying out an enzymatic reaction, which comprises: continuously supplying reducing equivalents to regenerate a reduce enzyme species in enzymatically active form, said reducing equivalents being derived electrochemically and passed directly to said enzyme from the cathode of an electrochemical system and taken-up and utilized by an electron receptor component of said enzyme.
Claim 3
Dependent Claim
71 words
A process for the oxidative or reductive transformation of an organic compound, comprising: said transformation being catalyzed by an enzyme and the enzyme requiring a continuing supply of reducing equivalents to regenerate a reduced enzyme species in enzymatically active form, said reducing equivalents being derived electrochemically and passed directly to the enzyme from the cathode of an electrochemical system and taken-up and utilized by an electron receptor component of the enzyme.
Claim 4
Dependent Claim
71 words
A process for the oxidative or reductive transformation of an organic compound, comprising: said transformation being catalyzed by an enzyme and the enzyme requiring a continuing supply of reducing equivalents to regenerate a reduced enzyme species in enzymatically active form, said reducing equivalents being derived electrochemically and passed directly to the enzyme from the cathode of an electrochemical system and taken-up and utilized by an electron receptor component of the enzyme.
Claim 5
Dependent Claim
27 words
The process of claim 1 or 2, wherein the enzyme comprises an external mono-oxygenase, an oxidoreductase, a dehydrogenase, a nitrogenase, a dioxygenase, a luciferase or a hydrogenase.
Claim 6
Dependent Claim
42 words
The process of claim 1 or 2, wherein the working electrode of the electrochemical system comprises a material which is inert to the enzyme species which it is desired to regenerate electrochemically and the auxiliary electrode is separated from the working electrode.
Claim 7
Dependent Claim
22 words
The process of claim 1 or 2, wherein the working electrode comprises a liquid crystal coated, semi-conductor or hanging mercury drop electrode.
Claim 8
Dependent Claim
15 words
The process of claim 1 or 2, wherein the electrolyte contains an electron transfer promoter.
Claim 9
Dependent Claim
34 words
The process according to claim 1 or 2, wherein the potential of the working electrode is controlled at or close to the redox potential of the enzyme species which it is desired to reduce.
Claim 10
Dependent Claim
31 words
The process of claim 7, wherein control of the potential of the working electrode is facilitated by use of a reference electrode located within the working region of the electrochemical cell.
Claim 11
Dependent Claim
20 words
The process of claim 1 or 2, wherein the enzyme is present as a component of a crude cell extract.
Claim 12
Dependent Claim
31 words
The process of claim 1 or 2, wherein said reducing equivalents are supplied to a primary solution containing said enzyme species electrochemically by the oxidation of hydrogen in a secondary solution.
Claim 13
Dependent Claim
26 words
The process of claim 1 or 2, wherein a hydrocarbon material is oxidized to an alcohol using a mono-oxygenase enzyme system derived from methane oxidizing bacteria.
Claim 14
Dependent Claim
12 words
The process of claim 11, wherein said mono-oxygenase enzyme is Methylosinus trichosporium.
Claim 15
Dependent Claim
21 words
The process of claim 1 or 2, wherein camphor is oxidized to hydroxycamphor by an enzyme system derived from Pseudomonas putida.
References Cited (Backward Citations)
No citation records indexed.
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1985-03-08 | 1985-09-07 | 1986-03-09 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1989-03-08 | 1989-09-07 | 1990-03-09 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1993-03-08 | 1993-09-07 | 1994-03-09 | $7700.00 | Window Closed / Lapsed |