US4312979A
Expired / Lapsed
US
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Polysaccharides containing allose
Assignee: Tosoh Corp
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Filing: 1979-04-16
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Granted: 1982-01-26
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Expires: 1999-04-16
Abstract
Abstract A new polysaccharide including allose as a constituent sugar and further characterized by galactose as a major constituent sugar is described. The polysaccharide is produced extracellularly by cultivation of Pseudomonas viscogena strains in nutrient medium.
Inventors
- 👤 Hisao Takemoto
- 👤 Tatsuo Igarashi
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-31 12:43
CPC Classifications
C; C08; C08B; C08B37/00; C12; C12P; C12P19/00; C12P19/04
Claims Analysis
Total Claims: 7
Claim 1
Independent Claim
207 words
A polysaccharide containing allose as a constituent sugar, said polysaccharide having the following identifying characteristics: (a) a molecular weight ranging from about 1×10 4 to about 1×10 7 ; (b) no clear melting point; (c) a content of from about 6 to about 12 mole % allose, from about 50 to about 60 mole % galactose, from about 10 to about 20 mole % glucose, from about 7 to about 14 mole % mannose and from 10 to about 12 mole % glucuronic acid; (d) infra-red spectra showing O-H stretching in the vicinity of 3,400 cm -1 , C--H stretching in the vicinity of 2,890 cm -1 and stretching of carboxylate anions in the vicinity of 1,620 cm -1 due to the orientation of beta-glucosido linkages in the vicinity of 800 cm -1 ; (e) no distinct characteristic ultraviolet absorption spectra; (f) solubility in water but insolubility in methanol, ethanol, ethers and acetone; (g) the following color reactions: anthrone reaction--positive ninhydrin reaction--negative Dische's carbazole reaction--positive Dische's cysteine-sulfuric acid reaction--positive; (h) a specific rotation in water [α] D 25 of from about +30° C.; (i) forms a colorless, transparent and viscous solution with water; and, (j) is aggregated to a gel in an aqueous solution containing calcium ion.
Claim 2
Dependent Claim
207 words
A polysaccharide containing allose as a constituent sugar, said polysaccharide having the following identifying characteristics: (a) a molecular weight ranging from about 1×10 4 to about 1×10 7 ; (b) no clear melting point; (c) a content of from about 6 to about 12 mole % allose, from about 50 to about 60 mole % galactose, from about 10 to about 20 mole % glucose, from about 7 to about 14 mole % mannose and from 10 to about 12 mole % glucuronic acid; (d) infra-red spectra showing O-H stretching in the vicinity of 3,400 cm -1 , C--H stretching in the vicinity of 2,890 cm -1 and stretching of carboxylate anions in the vicinity of 1,620 cm -1 due to the orientation of beta-glucosido linkages in the vicinity of 800 cm -1 ; (e) no distinct characteristic ultraviolet absorption spectra; (f) solubility in water but insolubility in methanol, ethanol, ethers and acetone; (g) the following color reactions: anthrone reaction--positive ninhydrin reaction--negative Dische's carbazole reaction--positive Dische's cysteine-sulfuric acid reaction--positive; (h) a specific rotation in water [α] D 25 of from about +30° C.; (i) forms a colorless, transparent and viscous solution with water; and, (j) is aggregated to a gel in an aqueous solution containing calcium ion.
Claim 3
Dependent Claim
20 words
The polysaccharide according to claim 1, wherein the molecular weight ranges from about 1.0×10 5 to about 5×10 6 .
Claim 4
Dependent Claim
20 words
The polysaccharide according to claim 1, wherein the molecular weight ranges from about 1.0×10 4 to about 1.0×10 6 .
Claim 5
Dependent Claim
20 words
The polysaccharide according to claim 1, wherein the molecular weight ranges from about 2.0×10 4 to about 1.0×10 6 .
Claim 6
Dependent Claim
20 words
The polysaccharide according to claim 1, wherein the molecular weight ranges from about 3.0×10 4 to about 2×10 6 .
Claim 7
Dependent Claim
20 words
The polysaccharide according to claim 1, wherein the molecular weight ranges from about 1.0×10 4 to about 5×10 5 .
References Cited (Backward Citations)
US3711462A
Cited
US4186025A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1985-01-25 | 1985-07-27 | 1986-01-26 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1989-01-25 | 1989-07-27 | 1990-01-26 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1993-01-25 | 1993-07-27 | 1994-01-26 | $7700.00 | Window Closed / Lapsed |